Long-chain-fatty-acid--CoA ligase (PA3299)

Identification
Name: Long-chain-fatty-acid--CoA ligase
Synonyms:
  • Long-chain acyl-CoA synthetase
  • Acyl-CoA synthetase
Gene Name: fadD
Enzyme Class:
Biological Properties
General Function: Involved in catalytic activity
Specific Function: Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids
Cellular Location: Membrane; Peripheral membrane protein.
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Palmitic acid → Adenosine monophosphate + Hydrogen ion + Palmityl-CoA + Pyrophosphate
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Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Adenosine triphosphate + Palmitic acid + Coenzyme A ↔ Adenosine monophosphate + Palmityl-CoA + Pyrophosphate
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Thumb+Long-chain fatty acid+Thumb ↔ Thumb+Thumb+Acyl-CoA
Adenosine triphosphate + Long-chain fatty acid + Coenzyme A ↔ Adenosine monophosphate + Pyrophosphate + Acyl-CoA
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SMPDB Reactions:
Thumb+a 2,3,4- saturated fatty acid+Thumb → Thumb+Pyrophosphate+a 2,3,4-saturated fatty acyl CoA?
Coenzyme A + a 2,3,4- saturated fatty acid + Adenosine triphosphate → Adenosine monophosphate + Pyrophosphate + a 2,3,4-saturated fatty acyl CoA?
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PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Dodecanoate (N-C12:0) + Hydrogen ion → Adenosine monophosphate + Lauroyl-CoA + Hydrogen ion + Pyrophosphate
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Palmitic acid → Adenosine monophosphate + Hydrogen ion + Palmityl-CoA + Pyrophosphate
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Octadecanoate (N-C18:0) → Adenosine monophosphate + Hydrogen ion + Pyrophosphate + Stearoyl-CoA
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + tetradecanoate (n-C14:0) → Adenosine monophosphate + Hydrogen ion + Pyrophosphate + Tetradecanoyl-CoA
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Tetradecenoate (N-C14:1) → Adenosine monophosphate + Hydrogen ion + Pyrophosphate + (2E)-Tetradecenoyl-CoA
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Decanoate (N-C10:0) + Hydrogen ion → Adenosine monophosphate + Decanoyl-CoA (N-C10:0CoA) + Hydrogen ion + Pyrophosphate
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Caprylic acid → Adenosine monophosphate + Hydrogen ion + Octanoyl-CoA + Pyrophosphate
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Hexanoate (N-C6:0) → Adenosine monophosphate + Hydrogen ion + Hexanoyl-CoA + Pyrophosphate
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Octadecenoate (N-C18:1) → Adenosine monophosphate + Hydrogen ion + Octadecenoyl-CoA (N-C18:1CoA) + Pyrophosphate
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Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Adenosine triphosphate + Palmitic acid + Coenzyme A ↔ Adenosine monophosphate + Palmityl-CoA + Pyrophosphate
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Thumb+Long-chain fatty acid+Thumb ↔ Thumb+Thumb+Acyl-CoA
Adenosine triphosphate + Long-chain fatty acid + Coenzyme A ↔ Adenosine monophosphate + Pyrophosphate + Acyl-CoA
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Thumb+a 2,3,4- saturated fatty acid+Thumb → Thumb+Pyrophosphate+a 2,3,4-saturated fatty acyl CoA?
Coenzyme A + a 2,3,4- saturated fatty acid + Adenosine triphosphate → Adenosine monophosphate + Pyrophosphate + a 2,3,4-saturated fatty acyl CoA?
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Thumb
octanoate + ATP + coenzyme A → octanoyl-CoA + AMP + diphosphate
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Odd-Straight-Chain-234-Sat-FA+PAMDB110477+PAMDB110248 → Odd-Saturated-Fatty-Acyl-CoA+PAMDB110478+PAMDB110246
Odd-Straight-Chain-234-Sat-FA + ATP + coenzyme A → Odd-Saturated-Fatty-Acyl-CoA + AMP + diphosphate
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3-Methyl-Saturated-Fatty-Acids+PAMDB110248+PAMDB110477 → 3-Methyl-Saturated-Fatty-Acyl-CoA+PAMDB110478+PAMDB110246
3-Methyl-Saturated-Fatty-Acids + coenzyme A + ATP → 3-Methyl-Saturated-Fatty-Acyl-CoA + AMP + diphosphate
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PAMDB110756+PAMDB110248+PAMDB110477 → PAMDB120086+PAMDB110246+PAMDB110478
linoleate + coenzyme A + ATP → linoleoyl-CoA + diphosphate + AMP
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PAMDB110477+PAMDB120089+PAMDB110248 → PAMDB120004+PAMDB110246+PAMDB110478
ATP + α-linolenate + coenzyme A → α-linolenoyl-CoA + diphosphate + AMP
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PAMDB120163+PAMDB110248+PAMDB110477 → PAMDB110389+PAMDB110478+PAMDB110246
oleate + coenzyme A + ATP → oleoyl-CoA + AMP + diphosphate
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Long-Chain-Fatty-Acids+PAMDB110248+PAMDB110477 → Long-Chain-Acyl-CoAs+PAMDB110246+PAMDB110478
Long-Chain-Fatty-Acids + coenzyme A + ATP → Long-Chain-Acyl-CoAs + diphosphate + AMP
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PAMDB120150+PAMDB110477+PAMDB110248 → PAMDB120068+PAMDB110246+PAMDB110478
eicosapentaenoate + ATP + coenzyme A → eicosapentaenoyl-CoA + diphosphate + AMP
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CPD66-39+PAMDB110248+PAMDB110477 → Saturated-Fatty-Acyl-CoA+PAMDB110246+PAMDB110478
CPD66-39 + coenzyme A + ATP → Saturated-Fatty-Acyl-CoA + diphosphate + AMP
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PAMDB120312+PAMDB110477+PAMDB110248 → PAMDB120258+PAMDB110478+PAMDB110246
phytenate + ATP + coenzyme A → phytenoyl-CoA + AMP + diphosphate
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R2OH-Straight-Chain-234-Sat-FA+PAMDB110477+PAMDB110248 → R2-2OH-Straight-Chain-234-Sat-FA-CoA+PAMDB110478+PAMDB110246
R2OH-Straight-Chain-234-Sat-FA + ATP + coenzyme A → R2-2OH-Straight-Chain-234-Sat-FA-CoA + AMP + diphosphate
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PAMDB120463+PAMDB110248+PAMDB110477 → PAMDB120428+PAMDB110246+PAMDB110478
(15Z)-tetracosenoate + coenzyme A + ATP → (Z)-15-tetracosenoyl-CoA + diphosphate + AMP
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PAMDB120512+PAMDB110248+PAMDB110477 → PAMDB120505+PAMDB110246+PAMDB110478
palmitate + coenzyme A + ATP → palmitoyl-CoA + diphosphate + AMP
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PAMDB120561+PAMDB110248+PAMDB110477 → PAMDB120547+PAMDB110246+PAMDB110478
pristanate + coenzyme A + ATP → pristanoyl-CoA + diphosphate + AMP
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2-Me-Branched-234-Sat-FA+PAMDB110248+PAMDB110477 → 2-Me-Branched-234-Sat-Fatty-Acyl-CoA+PAMDB110246+PAMDB110478
2-Me-Branched-234-Sat-FA + coenzyme A + ATP → 2-Me-Branched-234-Sat-Fatty-Acyl-CoA + diphosphate + AMP
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Complex Reactions:
Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Dodecanoate (N-C12:0) + Hydrogen ion → Adenosine monophosphate + Lauroyl-CoA + Hydrogen ion + Pyrophosphate
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Octadecanoate (N-C18:0) → Adenosine monophosphate + Hydrogen ion + Pyrophosphate + Stearoyl-CoA
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + tetradecanoate (n-C14:0) → Adenosine monophosphate + Hydrogen ion + Pyrophosphate + Tetradecanoyl-CoA
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Tetradecenoate (N-C14:1) → Adenosine monophosphate + Hydrogen ion + Pyrophosphate + (2E)-Tetradecenoyl-CoA
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Decanoate (N-C10:0) + Hydrogen ion → Adenosine monophosphate + Decanoyl-CoA (N-C10:0CoA) + Hydrogen ion + Pyrophosphate
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Caprylic acid → Adenosine monophosphate + Hydrogen ion + Octanoyl-CoA + Pyrophosphate
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Hexanoate (N-C6:0) → Adenosine monophosphate + Hydrogen ion + Hexanoyl-CoA + Pyrophosphate
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Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Coenzyme A + Hydrogen ion + Octadecenoate (N-C18:1) → Adenosine monophosphate + Hydrogen ion + Octadecenoyl-CoA (N-C18:1CoA) + Pyrophosphate
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000541(2E)-Hexadecenoyl-CoAMetaboCard
PAMDB000542(2E)-Tetradecenoyl-CoAMetaboCard
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000140Caprylic acidMetaboCard
PAMDB000381Coenzyme AMetaboCard
PAMDB001612Decanoate (N-C10:0)MetaboCard
PAMDB000670Decanoyl-CoA (N-C10:0CoA)MetaboCard
PAMDB001617Dodecanoate (N-C12:0)MetaboCard
PAMDB001637Hexanoate (N-C6:0)MetaboCard
PAMDB000467Hexanoyl-CoAMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000516Lauroyl-CoAMetaboCard
PAMDB001822Myristic acidMetaboCard
PAMDB001745Octadecanoate (N-C18:0)MetaboCard
PAMDB001746Octadecenoate (N-C18:1)MetaboCard
PAMDB001668Octadecenoyl-CoA (N-C18:1CoA)MetaboCard
PAMDB000241Octanoyl-CoAMetaboCard
PAMDB000091Palmitic acidMetaboCard
PAMDB001837Palmitoleic acidMetaboCard
PAMDB000343Palmityl-CoAMetaboCard
PAMDB000633PyrophosphateMetaboCard
PAMDB001823Stearic acidMetaboCard
PAMDB001690Stearoyl-CoAMetaboCard
PAMDB001757tetradecanoate (n-C14:0)MetaboCard
PAMDB000409Tetradecanoyl-CoAMetaboCard
PAMDB001758Tetradecenoate (N-C14:1)MetaboCard
PAMDB001838Vaccenic acidMetaboCard
GO Classification:
Function
catalytic activity
Process
metabolic process
Gene Properties
Locus tag: PA3299
Strand: -
Entrez Gene ID: 882462
Accession: NP_251989.1
GI: 15598495
Sequence start: 3695511
Sequence End: 3697199
Sequence Length: 1688
Gene Sequence: 
>PA3299
ATGATCGAAAACTTCTGGAAGGACAAGTACCCAGCGGGTATTGCTGCCGAGATCAATCCCGACCAGTACCCGAACATCCTGTCGGTACTGAAGGAGTCCTGCCAACGTTTTGCCACCAAGCCCGCGTTCACCAACCTGGGCAAGACCCTGACCTACGGCGAGCTGTACAAGCTGTCCGGCGACTTCGCCGCCTACCTGCAGCAGCACACCGATCTCAAGCCCGGCGACCGCATCGCCGTCCAGTTGCCCAACGTCCTGCAATACCCGATCGTGGTCTTCGGCGCCATGCGCGCCGGCCTCATCGTGGTCAACACCAACCCGCTGTACACCGCGCGCGAACTGGAACACCAGTTCAACGACTCCGGCGCCAAGGCGGTGGTCTGCCTGGCCAACATGGCCCACCTGGTCGAGGGCGTACTGCCGAAGACCGGCGTCAAGCAGGTGATCGTCACCGAAGTCGGCGACATCCTGCCGCCGCTGAAGCGCTTCATCGTCAACTTCGTGGTCAAGCACATCAAGAAGATGGTGCCGGCCTACAGCCTGCCGCAAGCCACCAAGCTGACCGACGCGCTGGCCAGGGGCGCCGGCAAGTCGTTCCAGGAAGCCGCGCCGCAGGCCGACGACGTCGCCGTGCTGCAATACACCGGCGGCACCACCGGGGTCGCCAAGGGCGCCATGCTGACCCACCGCAACCTGGTGGCCAACATGTTGCAGTGCAAGGCGCTGATGGGCGCCAACCTCAACGAGGGCTGCGAGATCCTCATCGCGCCGCTGCCGCTGTACCACATCTATGCCTTCACCTTCCATTGCATGGCGATGATGCTCACCGGCAACCACAACATCCTGATCACCAACCCGCGCGACCTGCCGTCGATGCTCAAGGACCTCGGCCAGTGGAAGTTCACCGGCTTCGTCGGCCTGAACACCCTGTTCGTCGCCCTGTGCAACAACGAAACCTTCCGCAAGCTGGACTTCTCCGCGCTGAAGCTGACCCTCTCCGGCGGCATGGCGCTGCAACTGGCCACCGCGGAGCGCTGGAAGGAAGTCACCGGCTGCGCCATCTGCGAAGGCTACGGCATGACCGAGACCGCGCCGGTGGTATCGGTCAACCCGTTCCAGAACATCCAGGTCGGCACCATCGGTATCCCGGTGCCCTCGACCCTGTGCAAGGTGATTGGCGACGACGGCCAGGAAGTGCCCCTGGGCGAGCGCGGCGAACTCTGCGTGAAGGGTCCGCAGGTGATGAAGGGCTACTGGCAGCGCCAGGAGGCCACCGACGAGATCCTCGACGCCGATGGTTGGCTGAAGACCGGCGATATCGCCATCATCCAGGAAGACGGCTACATGCGCATCGTCGACCGGAAGAAGGACATGATCCTGGTCTCCGGCTTCAATGTGTATCCGAACGAACTGGAAGACGTGCTGGCGACCCTGCCGGGCGTCCTGCAGTGCGCCGCGATCGGCATCCCCGACGAGAAGTCCGGCGAGTCGATCAAGGTCTTCGTGGTGGTCAAGCCGGGGGCGACCCTGACCAAGGAACAGGTCATGCAGCACATGCATGACAACCTGACCGGCTACAAGCGGCCGAAGGCCGTGGAGTTCCGCGACAGCCTGCCGACCACCAACGTCGGCAAGATCCTGCGCCGCGAACTGCGCGACGAAGAGCTGAAGAAAGCGGGCCAGAAGTAA
Protein Properties
Protein Residues: 562
Protein Molecular Weight: 61.7 kDa
Protein Theoretical pI: 7.45
Hydropathicity (GRAVY score): -0.027
Charge at pH 7 (predicted): 2.08
Protein Sequence: 
>PA3299
MIENFWKDKYPAGIAAEINPDQYPNILSVLKESCQRFATKPAFTNLGKTLTYGELYKLSGDFAAYLQQHTDLKPGDRIAVQLPNVLQYPIVVFGAMRAGLIVVNTNPLYTARELEHQFNDSGAKAVVCLANMAHLVEGVLPKTGVKQVIVTEVGDILPPLKRFIVNFVVKHIKKMVPAYSLPQATKLTDALARGAGKSFQEAAPQADDVAVLQYTGGTTGVAKGAMLTHRNLVANMLQCKALMGANLNEGCEILIAPLPLYHIYAFTFHCMAMMLTGNHNILITNPRDLPSMLKDLGQWKFTGFVGLNTLFVALCNNETFRKLDFSALKLTLSGGMALQLATAERWKEVTGCAICEGYGMTETAPVVSVNPFQNIQVGTIGIPVPSTLCKVIGDDGQEVPLGERGELCVKGPQVMKGYWQRQEATDEILDADGWLKTGDIAIIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLATLPGVLQCAAIGIPDEKSGESIKVFVVVKPGATLTKEQVMQHMHDNLTGYKRPKAVEFRDSLPTTNVGKILRRELRDEELKKAGQK
References
External Links:
Resource Link
Genome ID: PA3299
Entrez Gene ID: 882462
NCBI Protein ID: 15598495
General Reference: PaperBLAST - Find papers about PA3299 and its homologs