Identification
Name: Periplasmic nitrate reductase
Synonyms: Not Available
Gene Name: napA
Enzyme Class:
Biological Properties
General Function: Involved in oxidoreductase activity
Specific Function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism
Cellular Location: Periplasm
KEGG Pathways:
KEGG Reactions:
Thumb+Acceptor+Thumb+AcceptorThumb+Reduced acceptor+Reduced acceptor
Nitrite + Acceptor + Water + Acceptor ↔ Nitrate + Reduced acceptor + Reduced acceptor
ReactionCard
2 Ferricytochrome c+Thumb+ThumbThumb+2 Ferrocytochrome c+2 Thumb
2 Ferricytochrome c + Nitrite + WaterNitrate + 2 Ferrocytochrome c + 2 Hydrogen ion
ReactionCard
SMPDB Reactions:
Nitrate+cytochrome c nitrite reductase+ThumbNitrite+cytochrome c nitrite reductase+Thumb+Thumb
Nitrate + cytochrome c nitrite reductase + Nitrate ↔ Nitrite + cytochrome c nitrite reductase + Water + Nitrite
ReactionCard
Thumb+2 Thumb+2 ElectronThumb+Thumb
Thumb+2 Thumb+"a menaquinol"Thumb+Thumb+"a menaquinone"
Nitrate + 2 Hydrogen ion + "a menaquinol" → Nitrite + Water + "a menaquinone"
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Acceptor+Thumb+AcceptorThumb+Reduced acceptor+Reduced acceptor
Nitrite + Acceptor + Water + Acceptor ↔ Nitrate + Reduced acceptor + Reduced acceptor
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2 Ferricytochrome c+Thumb+ThumbThumb+2 Ferrocytochrome c+2 Thumb
2 Ferricytochrome c + Nitrite + WaterNitrate + 2 Ferrocytochrome c + 2 Hydrogen ion
ReactionCard
Thumb+acceptorThumb+reduced acceptor
Nitrite + acceptor → Nitrate + reduced acceptor
ReactionCard
Nitrate+cytochrome c nitrite reductase+ThumbNitrite+cytochrome c nitrite reductase+Thumb+Thumb
Nitrate + cytochrome c nitrite reductase + Nitrate ↔ Nitrite + cytochrome c nitrite reductase + Water + Nitrite
ReactionCard
Thumb+2 Thumb+2 ElectronThumb+Thumb
Thumb
nitrite + an oxidized unknown electron acceptor + H2O = nitrate + an reduced unknown electron acceptor
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Menaquinones+PAMDB001633Menaquinols
Menaquinones + Hydrogen ion → Menaquinols
ReactionCard
PAMDB000468+PAMDB001633PAMDB000465+PAMDB000142
PAMDB000465+PAMDB000142+PAMDB110541PAMDB001633+PAMDB000468+PAMDB110539
PAMDB000468PAMDB000465
PAMDB000468+UbiquinolsPAMDB000465+PAMDB000142+Ubiquinones
Nitrate + Ubiquinols → Nitrite + Water + Ubiquinones
ReactionCard
PAMDB000468+PAMDB001633+MenaquinolsPAMDB000465+PAMDB000142+Menaquinones+PAMDB001633
Nitrate + Hydrogen ion + Menaquinols → Nitrite + Water + Menaquinones + Hydrogen ion
ReactionCard
PAMDB000465+PAMDB000142PAMDB000468
Complex Reactions:
2 Thumb+Thumb+ThumbThumb+Thumb+Thumb+2 Thumb
2 Thumb+Thumb+ThumbThumb+Menaquinone 8+Thumb+2 Thumb
Thumb+ThumbThumb+Thumb+Thumb
Thumb+ThumbMenaquinone 8+Thumb+Thumb
Thumb+acceptorThumb+reduced acceptor
Nitrite + acceptor → Nitrate + reduced acceptor
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB002046Ferricytochrome cMetaboCard
PAMDB003525Ferrocytochrome cMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001653Menaquinol 8MetaboCard
PAMDB000468NitrateMetaboCard
PAMDB000465NitriteMetaboCard
PAMDB004318SelenateMetaboCard
PAMDB001752SeleniteMetaboCard
PAMDB000237Ubiquinol-8MetaboCard
PAMDB001700Ubiquinone-8MetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Component
cell part
periplasmic space
Function
4 iron, 4 sulfur cluster binding
binding
catalytic activity
cation binding
electron carrier activity
ion binding
iron-sulfur cluster binding
metal cluster binding
metal ion binding
molybdenum ion binding
nitrate reductase activity
oxidoreductase activity
oxidoreductase activity, acting on other nitrogenous compounds as donors
transition metal ion binding
Process
metabolic process
nitrate assimilation
nitrate metabolic process
nitrogen compound metabolic process
oxidation reduction
Gene Properties
Locus tag: PA1174
Strand: -
Entrez Gene ID: 878409
Accession: NP_249865.1
GI: 15596371
Sequence start: 1273309
Sequence End: 1275798
Sequence Length: 2489
Gene Sequence:
>PA1174
GTGAATTCGCCAAGGCCAACGCCGCCGCCATTCGCCGCGGCGGCGGCCGGCCTGCCGATCCTGGTCCGCGCCAGCAACCTGGTGACCGAGGCGGACGTCACCAGCCTGGTCTGGAACAAGGCCCCCTGCCGCTTCTGCGGCACCGGTTGCAGCGTGATGGTCGCCACCCGCGACGGCCAGGTGGTGGCCACCCATGGCGACATCAAGGCCGAGGTCAACCGCGGGATCAACTGCGTGAAGGGCTACTTCCTGTCGAAGATCATGTACGGCAGCGACCGCCTGACCCGCCCGCTGCTGCGCATGAAGGACGGCAAGTTCGACAAGCAGGGCGAGTTCCAGCCGATCAGTTGGGAACAGGCCTTCGACATCATGGCCGAGAAGTTCAAGGCCGCGCTCAAGGCCAAGGGACCGGAGTCGGTCGGCATGTTCGGCTCCGGCCAGTGGACGGTCTGGGAAGGCTATGCGGCGAACAAGCTGTTCAAGGCCGGCCTGCGCAGCAACAACATCGACCCCAACGCCCGCCACTGCATGGCCTCGGCGGTGATGGGCTTCATGCGCAGCTTCGGCATGGACGAGCCGATGGGCTGCTACGACGACATCGAGGCCACCGACAGCTTCGTCCTCTGGGGCTCGAACATGGCCGAGATGCACCCGGTGCTCTGGTCGCGGGTCACCGACCGCCGGCTCAGCGCGCCGCAGGTCAAGGTCGCGGTGCTGTCCACCTTCGAGCACCGCAGCTTCGAGCTGGCCGACCTGCCCATGGTGTTCAAGCCGCAGACCGACCTGATCATCCTCAACTACATCGCCAACCACATCATCGAGAGCGGCGCGGTGAACCGCGACTTCGTCGAGCGCCACGTGCGCTTCGCCCATGGCGCCGAGGACATCGGCTACGGCCTGCGCCCGGACGACCCGCTGGAGAAGAAGGCGAAGAACGCCGACAAGGCCAACACCTGGAGCGACATCGACTTCAAGGCCTTCGCCGAGTTCGTCAAGCCCTATACCCTGGAGCGCACCGCGCGCGAAAGCGGCGTGCCCGCCGAGCGGCTGAAGGCCCTCGCCGAACTCTACGCCGACCCCAAGCGCAAGGTGGTGTCGTTCTGGACCATGGGCTTCAACCAGCACACCCGCGGGGTCTGGGCGAACAACCTGATCTACAACATCCACCTGCTCACCGGGAAGATCAGCGAACCGGGCAACAGCCCCTTCTCGCTCACCGGCCAGCCCTCGGCCTGCGGCACCGCGCGCGAGGTCGGCACCTTCTCCCACCGGCTCCCCGCCGACCTGGTGGTGACCAACCCGAAGCACCGGGAAACCGCGGAGAAGATCTGGAAGGTGCCGGCCGGCACCATCCAGGAGAAGGTCGGCTTCCACGCCGTGCAGCAGAGCCGGATGCTCAAGGACGGCGTGCTCAACGTCTACTGGACCCAGGTCAGCAACAACATGCAGGCCGGTCCCAACGTGATGCAGGAAGTGCTGCCCGGCTGGCGCAACCCGGACAACTTCGTGATCGTCTCCGACGTCTACCCCACCGTCTCGGCCCAGGCCGCCGACCTGATCCTGCCCAGCGCCATGTGGGTGGAGAAGGAAGGCGCCTTCGGCAATGCCGAGCGCCGCACGCAGTTCTGGCACCAGTTGGTGAAGGCACCGGGCGAGGCGAAGTCCGACCTCTGGCAGCTGGTGGAATTCTCCAAGCGCTTCACCACCGACGAGGTCTGGCCCGCGGAACTGCTGGCGAAGGCCCCCGAGCTGAAGGGCAAGACCCTCTACGACGTGCTGTTCCGCAACGGCCAGGTCGACCGCTTCCCGGCCAGCGACCTGGCCAAGGGCTATGCCAACGACGAGGTCGACGCATTCGGCTTCTATATCCAGAAGGGTCTGTTCGAGGAATACGCCGCCTTCGGTCGCGGCCACGGCCACGACCTCGCCCCCTTCGACGCCTACCACGAGGCGCGCGGCCTGCGCTGGCCGGTGGTGGACGGCAAGGAGACCCGCTGGCGCTACCGCGAGGGCTACGACCCCTACGTGAGCAAGGGCAGCGGCGTGCAGTTCTATGGCTATCCGGACAAGAAGGCGATCGTCTTCGCCCTGCCCTACGAGCCGCCGGCCGAGGCGCCGGACCAGGACTACCCGTTCTGGCTCGCCACCGGCCGGGTGCTCGAACACTGGCACACCGGGAGCATGACCGCGCGGGTCCCGGAGCTGTACAAGGCGGTGCCCGACGCGCTGGTCTACATGCACCCGGAGGATGCGCGCCAGCTCAAGCTGCGCCGCGGCAGCGAGGTCAAGGTGGTGAGCCGGCGCGGCGAGATCCGCGCGCGGGTCGAGACCCGCGGGCGCAACAAGCCGCCCCAGGGGCTGGTGTTCGTGCCGTTCTTCGACGCCAACAAGCTGATCAACAAGGTCACCCTGGACGCCACCGACCCGATTTCCAAGCAGACCGACTACAAGAAGTGCGCCGTTCGCATCGAACTGCTCAACCTGGCCTGA
Protein Properties
Protein Residues: 829
Protein Molecular Weight: 92.9 kDa
Protein Theoretical pI: 8.61
Hydropathicity (GRAVY score): -0.359
Charge at pH 7 (predicted): 10.37
Protein Sequence:
>PA1174
MNSPRPTPPPFAAAAAGLPILVRASNLVTEADVTSLVWNKAPCRFCGTGCSVMVATRDGQVVATHGDIKAEVNRGINCVKGYFLSKIMYGSDRLTRPLLRMKDGKFDKQGEFQPISWEQAFDIMAEKFKAALKAKGPESVGMFGSGQWTVWEGYAANKLFKAGLRSNNIDPNARHCMASAVMGFMRSFGMDEPMGCYDDIEATDSFVLWGSNMAEMHPVLWSRVTDRRLSAPQVKVAVLSTFEHRSFELADLPMVFKPQTDLIILNYIANHIIESGAVNRDFVERHVRFAHGAEDIGYGLRPDDPLEKKAKNADKANTWSDIDFKAFAEFVKPYTLERTARESGVPAERLKALAELYADPKRKVVSFWTMGFNQHTRGVWANNLIYNIHLLTGKISEPGNSPFSLTGQPSACGTAREVGTFSHRLPADLVVTNPKHRETAEKIWKVPAGTIQEKVGFHAVQQSRMLKDGVLNVYWTQVSNNMQAGPNVMQEVLPGWRNPDNFVIVSDVYPTVSAQAADLILPSAMWVEKEGAFGNAERRTQFWHQLVKAPGEAKSDLWQLVEFSKRFTTDEVWPAELLAKAPELKGKTLYDVLFRNGQVDRFPASDLAKGYANDEVDAFGFYIQKGLFEEYAAFGRGHGHDLAPFDAYHEARGLRWPVVDGKETRWRYREGYDPYVSKGSGVQFYGYPDKKAIVFALPYEPPAEAPDQDYPFWLATGRVLEHWHTGSMTARVPELYKAVPDALVYMHPEDARQLKLRRGSEVKVVSRRGEIRARVETRGRNKPPQGLVFVPFFDANKLINKVTLDATDPISKQTDYKKCAVRIELLNLA
References
External Links:
Resource Link
Genome ID: PA1174
Entrez Gene ID: 878409
NCBI Protein ID: 15596371
General Reference: PaperBLAST - Find papers about PA1174 and its homologs