| Identification |
| Name: |
Periplasmic nitrate reductase |
| Synonyms: |
Not Available |
| Gene Name: |
napA |
| Enzyme Class: |
|
| Biological Properties |
| General Function: |
Involved in oxidoreductase activity |
| Specific Function: |
Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism |
| Cellular Location: |
Periplasm |
| KEGG Pathways: |
- Microbial metabolism in diverse environments pae01120
- Nitrogen metabolism pae00910
|
| KEGG Reactions: |
|
 | + | Acceptor | + |  | + | Acceptor | ↔ |  | + | Reduced acceptor | + | Reduced acceptor |
| | |
2 Ferricytochrome c | + | | + | | ↔ | | + | 2 Ferrocytochrome c | + | 2  |
| |
|
| SMPDB Reactions: |
|
Nitrate | + | cytochrome c nitrite reductase | + | | ↔ | Nitrite | + | cytochrome c nitrite reductase | + | | + | |
| | | | |
| + | 2 | + | "a menaquinol" | → | | + | | + | "a menaquinone" |
| |
|
| PseudoCyc/BioCyc Reactions: |
|
| Complex Reactions: |
| | | | | | | | |
| + | acceptor | → | | + | reduced acceptor |
| |
|
| Transports: |
Not Available |
| Metabolites: |
|
| GO Classification: |
| Component |
|---|
| cell part | | periplasmic space | | Function |
|---|
| 4 iron, 4 sulfur cluster binding | | binding | | catalytic activity | | cation binding | | electron carrier activity | | ion binding | | iron-sulfur cluster binding | | metal cluster binding | | metal ion binding | | molybdenum ion binding | | nitrate reductase activity | | oxidoreductase activity | | oxidoreductase activity, acting on other nitrogenous compounds as donors | | transition metal ion binding | | Process |
|---|
| metabolic process | | nitrate assimilation | | nitrate metabolic process | | nitrogen compound metabolic process | | oxidation reduction |
|
| Gene Properties |
| Locus tag: |
PA1174 |
| Strand: |
- |
| Entrez Gene ID: |
878409 |
| Accession: |
NP_249865.1 |
| GI: |
15596371 |
| Sequence start: |
1273309 |
| Sequence End: |
1275798 |
| Sequence Length: |
2489 |
| Gene Sequence: |
>PA1174
GTGAATTCGCCAAGGCCAACGCCGCCGCCATTCGCCGCGGCGGCGGCCGGCCTGCCGATCCTGGTCCGCGCCAGCAACCTGGTGACCGAGGCGGACGTCACCAGCCTGGTCTGGAACAAGGCCCCCTGCCGCTTCTGCGGCACCGGTTGCAGCGTGATGGTCGCCACCCGCGACGGCCAGGTGGTGGCCACCCATGGCGACATCAAGGCCGAGGTCAACCGCGGGATCAACTGCGTGAAGGGCTACTTCCTGTCGAAGATCATGTACGGCAGCGACCGCCTGACCCGCCCGCTGCTGCGCATGAAGGACGGCAAGTTCGACAAGCAGGGCGAGTTCCAGCCGATCAGTTGGGAACAGGCCTTCGACATCATGGCCGAGAAGTTCAAGGCCGCGCTCAAGGCCAAGGGACCGGAGTCGGTCGGCATGTTCGGCTCCGGCCAGTGGACGGTCTGGGAAGGCTATGCGGCGAACAAGCTGTTCAAGGCCGGCCTGCGCAGCAACAACATCGACCCCAACGCCCGCCACTGCATGGCCTCGGCGGTGATGGGCTTCATGCGCAGCTTCGGCATGGACGAGCCGATGGGCTGCTACGACGACATCGAGGCCACCGACAGCTTCGTCCTCTGGGGCTCGAACATGGCCGAGATGCACCCGGTGCTCTGGTCGCGGGTCACCGACCGCCGGCTCAGCGCGCCGCAGGTCAAGGTCGCGGTGCTGTCCACCTTCGAGCACCGCAGCTTCGAGCTGGCCGACCTGCCCATGGTGTTCAAGCCGCAGACCGACCTGATCATCCTCAACTACATCGCCAACCACATCATCGAGAGCGGCGCGGTGAACCGCGACTTCGTCGAGCGCCACGTGCGCTTCGCCCATGGCGCCGAGGACATCGGCTACGGCCTGCGCCCGGACGACCCGCTGGAGAAGAAGGCGAAGAACGCCGACAAGGCCAACACCTGGAGCGACATCGACTTCAAGGCCTTCGCCGAGTTCGTCAAGCCCTATACCCTGGAGCGCACCGCGCGCGAAAGCGGCGTGCCCGCCGAGCGGCTGAAGGCCCTCGCCGAACTCTACGCCGACCCCAAGCGCAAGGTGGTGTCGTTCTGGACCATGGGCTTCAACCAGCACACCCGCGGGGTCTGGGCGAACAACCTGATCTACAACATCCACCTGCTCACCGGGAAGATCAGCGAACCGGGCAACAGCCCCTTCTCGCTCACCGGCCAGCCCTCGGCCTGCGGCACCGCGCGCGAGGTCGGCACCTTCTCCCACCGGCTCCCCGCCGACCTGGTGGTGACCAACCCGAAGCACCGGGAAACCGCGGAGAAGATCTGGAAGGTGCCGGCCGGCACCATCCAGGAGAAGGTCGGCTTCCACGCCGTGCAGCAGAGCCGGATGCTCAAGGACGGCGTGCTCAACGTCTACTGGACCCAGGTCAGCAACAACATGCAGGCCGGTCCCAACGTGATGCAGGAAGTGCTGCCCGGCTGGCGCAACCCGGACAACTTCGTGATCGTCTCCGACGTCTACCCCACCGTCTCGGCCCAGGCCGCCGACCTGATCCTGCCCAGCGCCATGTGGGTGGAGAAGGAAGGCGCCTTCGGCAATGCCGAGCGCCGCACGCAGTTCTGGCACCAGTTGGTGAAGGCACCGGGCGAGGCGAAGTCCGACCTCTGGCAGCTGGTGGAATTCTCCAAGCGCTTCACCACCGACGAGGTCTGGCCCGCGGAACTGCTGGCGAAGGCCCCCGAGCTGAAGGGCAAGACCCTCTACGACGTGCTGTTCCGCAACGGCCAGGTCGACCGCTTCCCGGCCAGCGACCTGGCCAAGGGCTATGCCAACGACGAGGTCGACGCATTCGGCTTCTATATCCAGAAGGGTCTGTTCGAGGAATACGCCGCCTTCGGTCGCGGCCACGGCCACGACCTCGCCCCCTTCGACGCCTACCACGAGGCGCGCGGCCTGCGCTGGCCGGTGGTGGACGGCAAGGAGACCCGCTGGCGCTACCGCGAGGGCTACGACCCCTACGTGAGCAAGGGCAGCGGCGTGCAGTTCTATGGCTATCCGGACAAGAAGGCGATCGTCTTCGCCCTGCCCTACGAGCCGCCGGCCGAGGCGCCGGACCAGGACTACCCGTTCTGGCTCGCCACCGGCCGGGTGCTCGAACACTGGCACACCGGGAGCATGACCGCGCGGGTCCCGGAGCTGTACAAGGCGGTGCCCGACGCGCTGGTCTACATGCACCCGGAGGATGCGCGCCAGCTCAAGCTGCGCCGCGGCAGCGAGGTCAAGGTGGTGAGCCGGCGCGGCGAGATCCGCGCGCGGGTCGAGACCCGCGGGCGCAACAAGCCGCCCCAGGGGCTGGTGTTCGTGCCGTTCTTCGACGCCAACAAGCTGATCAACAAGGTCACCCTGGACGCCACCGACCCGATTTCCAAGCAGACCGACTACAAGAAGTGCGCCGTTCGCATCGAACTGCTCAACCTGGCCTGA |
| Protein Properties |
| Protein Residues: |
829 |
| Protein Molecular Weight: |
92.9 kDa |
| Protein Theoretical pI: |
8.61 |
| Hydropathicity (GRAVY score): |
-0.359 |
| Charge at pH 7 (predicted): |
10.37 |
| Protein Sequence: |
>PA1174
MNSPRPTPPPFAAAAAGLPILVRASNLVTEADVTSLVWNKAPCRFCGTGCSVMVATRDGQVVATHGDIKAEVNRGINCVKGYFLSKIMYGSDRLTRPLLRMKDGKFDKQGEFQPISWEQAFDIMAEKFKAALKAKGPESVGMFGSGQWTVWEGYAANKLFKAGLRSNNIDPNARHCMASAVMGFMRSFGMDEPMGCYDDIEATDSFVLWGSNMAEMHPVLWSRVTDRRLSAPQVKVAVLSTFEHRSFELADLPMVFKPQTDLIILNYIANHIIESGAVNRDFVERHVRFAHGAEDIGYGLRPDDPLEKKAKNADKANTWSDIDFKAFAEFVKPYTLERTARESGVPAERLKALAELYADPKRKVVSFWTMGFNQHTRGVWANNLIYNIHLLTGKISEPGNSPFSLTGQPSACGTAREVGTFSHRLPADLVVTNPKHRETAEKIWKVPAGTIQEKVGFHAVQQSRMLKDGVLNVYWTQVSNNMQAGPNVMQEVLPGWRNPDNFVIVSDVYPTVSAQAADLILPSAMWVEKEGAFGNAERRTQFWHQLVKAPGEAKSDLWQLVEFSKRFTTDEVWPAELLAKAPELKGKTLYDVLFRNGQVDRFPASDLAKGYANDEVDAFGFYIQKGLFEEYAAFGRGHGHDLAPFDAYHEARGLRWPVVDGKETRWRYREGYDPYVSKGSGVQFYGYPDKKAIVFALPYEPPAEAPDQDYPFWLATGRVLEHWHTGSMTARVPELYKAVPDALVYMHPEDARQLKLRRGSEVKVVSRRGEIRARVETRGRNKPPQGLVFVPFFDANKLINKVTLDATDPISKQTDYKKCAVRIELLNLA |
| References |
| External Links: |
|
| General Reference: |
PaperBLAST - Find papers about PA1174 and its homologs
|
