Glutamate--cysteine ligase (PA5203)

Identification
Name: Glutamate--cysteine ligase
Synonyms:
  • Gamma-ECS
  • GCS
  • Gamma-glutamylcysteine synthetase
Gene Name: gshA
Enzyme Class:
Biological Properties
General Function: Involved in glutamate-cysteine ligase activity
Specific Function: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + L-Cysteine + L-Glutamate ↔ ADP + gamma-Glutamylcysteine + Hydrogen ion + Phosphate
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Adenosine triphosphate + L-Glutamate + L-Cysteine ↔ ADP + Phosphate + gamma-Glutamylcysteine
ReactionCard
SMPDB Reactions:
L-Glutamic acid+Thumb+Thumb+Thumb → Adenosine diphosphate+Thumb+Thumb+Thumb+Thumb
L-Glutamic acid + Adenosine triphosphate + L-Cysteine + L-Glutamate → Adenosine diphosphate + Phosphate + Hydrogen ion + gamma-Glutamylcysteine + ADP
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + L-Cysteine + L-Glutamate ↔ ADP + gamma-Glutamylcysteine + Hydrogen ion + Phosphate
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
L-Cysteine + L-Glutamate + Adenosine triphosphate → Hydrogen ion + gamma-Glutamylcysteine + Phosphate + ADP
ReactionCard
Complex Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
Adenosine triphosphate + L-Glutamate + L-Cysteine → ADP + Inorganic phosphate + gamma-Glutamylcysteine
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000345ADPMetaboCard
PAMDB000232gamma-GlutamylcysteineMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001776Inorganic phosphateMetaboCard
PAMDB000152L-CysteineMetaboCard
PAMDB000055L-GlutamateMetaboCard
PAMDB000383PhosphateMetaboCard
GO Classification:
Function
acid-amino acid ligase activity
catalytic activity
glutamate-cysteine ligase activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
Process
cellular metabolic process
glutathione biosynthetic process
glutathione metabolic process
metabolic process
peptide metabolic process
Gene Properties
Locus tag: PA5203
Strand: +
Entrez Gene ID: 879474
Accession: NP_253890.1
GI: 15600396
Sequence start: 5856532
Sequence End: 5858115
Sequence Length: 1583
Gene Sequence: 
>PA5203
TTGAGCGATCTTCTCTCCCGCCGCCTGGCTCTGCTCGGCGCCGCAGCCAACCTGCCCCTGCTGACCGAGTGCCTGCATGGCATCGAGCGCGAATGCCTGCGAGTCGATAGCGACGGCAAGCTGGCGCTCACCCCCCACCCCCGGGCCCTGGGTTCGACCCTGACCCATCCGCAGATCACTACCGACTACTCCGAGGCCTTGCTGGAGTTCATCACGCCCACGGAAACCGACGTCGCCGACACTCTGGGCGATCTCGAGCGCATCCACCGCTTCGCCAGTTCGAAACTGGACGGCGAATACCTGTGGAGCCCATCGATGCCCTGCGAGCTGCCGGACGAGGAGAGCATCCCGATCGCCCGCTACGGCAGCTCGATGATCGGCCGCCTGAAATACGTCTACCGCAAGGGCCTGGCGCTGCGCTACGGCAAGACCATGCAGTGCATCGCCGGCATCCACTACAACTTCTCCCTGCCGGAGAGGCTCTGGCCGCTGCTGCGCCAGGCCGAAGGCAGCGAGCTGTCGGAACGCGACTACCAGTCCGCCGCCTACATCGCCCTGATTCGCAATTTCCGCCGCTACAGCTGGCTGCTGATGTACCTGTTCGGCGCCTCGCCGGCCCTCGACGCCGGCTTCCTGCGTGGCCGCCCGAGCCAGTTGGAGCGCCTCGACGAACACACGCTTTACCTGCCCTACGCCACCAGCCTGCGGATGAGCGACCTGGGCTACCAGAACAACGCCCAGGCCGGCCTGACGCCCTGCTACAACGACCTGCAGAGCTACATCGACAGCCTGCGCCAGGCGGTCAGCACGCCCTACCCGCCCTACGAGAAGGTCGGCACCAAGCAGGATGGCGAATGGGTGCAGCTGAACACCAACATCCTGCAGATCGAGAACGAGTACTACTCGAGCATCCGGCCAAAGCGCGTCACCTACACCGGCGAGCGTCCGGTGCAGGCCCTGGCCGCTCGCGGCGTGCAGTACGTGGAAGTGCGCTGCCTGGACATCAACCCGTTCCTGCCGCTGGGCATCGACCTGGACGAGGCGCGCTTCCTCGACGCCTTCCTGCTGTTCTGCGCGTTCAGCGACAGCCCGCTGCTGAACGGCGAGTGCAGCGACGCCACCGACAACTTCCTCGCCGTGGTCAAGGAAGGCAGGCGGCCGGGCCTGCAATTGCAACGTCGCGGCCAGCCGGTGGAATTGCAGGTCTGGGCGAACGAGCTGCTCGAACGAATCGCCGACACCGCGGCGCTGCTCGACCGCGCGCGCGGCGGCGAAGCCCACGCGGCGGCGCTGGCAGCGCAGCGGGCGAAGGTGGCCGATGCGGAACTGACGCCTTCGGCGCAGGTGCTGAAGGTCATGCGCGAGCGCGGCGAGAGCTTCGAAGCGTTCTCCCTGCGCCAGAGCCGCGAACACGCGGAGTATTTCCGCCAGCATCCGCTGGCCGCCGAAGAGCAGGCGCGCTTCGAGAAGATGGCCAGCGACTCCCTGGCCGAACAGACGGAGCTGGAACGCGACCAGGATGGCGACTTCGATACCTTCGTCGCCGCCTACCAGGCGAGCATCCTCGGCCTGATCAGCAACTGA
Protein Properties
Protein Residues: 527
Protein Molecular Weight: 59.2 kDa
Protein Theoretical pI: 4.87
Hydropathicity (GRAVY score): -0.317
Charge at pH 7 (predicted): -16.05
Protein Sequence: 
>PA5203
MSDLLSRRLALLGAAANLPLLTECLHGIERECLRVDSDGKLALTPHPRALGSTLTHPQITTDYSEALLEFITPTETDVADTLGDLERIHRFASSKLDGEYLWSPSMPCELPDEESIPIARYGSSMIGRLKYVYRKGLALRYGKTMQCIAGIHYNFSLPERLWPLLRQAEGSELSERDYQSAAYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRPSQLERLDEHTLYLPYATSLRMSDLGYQNNAQAGLTPCYNDLQSYIDSLRQAVSTPYPPYEKVGTKQDGEWVQLNTNILQIENEYYSSIRPKRVTYTGERPVQALAARGVQYVEVRCLDINPFLPLGIDLDEARFLDAFLLFCAFSDSPLLNGECSDATDNFLAVVKEGRRPGLQLQRRGQPVELQVWANELLERIADTAALLDRARGGEAHAAALAAQRAKVADAELTPSAQVLKVMRERGESFEAFSLRQSREHAEYFRQHPLAAEEQARFEKMASDSLAEQTELERDQDGDFDTFVAAYQASILGLISN
References
External Links:
Resource Link
Genome ID: PA5203
Entrez Gene ID: 879474
NCBI Protein ID: 15600396
General Reference: PaperBLAST - Find papers about PA5203 and its homologs