Identification
Name: Respiratory nitrate reductase 1 beta chain
Synonyms:
  • Nitrate reductase A subunit beta
  • Quinol-nitrate oxidoreductase subunit beta
Gene Name: narH
Enzyme Class:
Biological Properties
General Function: Involved in iron-sulfur cluster binding
Specific Function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit
Cellular Location: Cell membrane; Peripheral membrane protein
KEGG Pathways:
KEGG Reactions:
Thumb+Acceptor+Thumb+AcceptorThumb+Reduced acceptor+Reduced acceptor
Nitrite + Acceptor + Water + Acceptor ↔ Nitrate + Reduced acceptor + Reduced acceptor
ReactionCard
2 Ferricytochrome c+Thumb+ThumbThumb+2 Ferrocytochrome c+2 Thumb
2 Ferricytochrome c + Nitrite + WaterNitrate + 2 Ferrocytochrome c + 2 Hydrogen ion
ReactionCard
SMPDB Reactions:
Nitrate+cytochrome c nitrite reductase+ThumbNitrite+cytochrome c nitrite reductase+Thumb+Thumb
Nitrate + cytochrome c nitrite reductase + Nitrate ↔ Nitrite + cytochrome c nitrite reductase + Water + Nitrite
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Thumb+2 Thumb+2 ElectronThumb+Thumb
Thumb+2 Thumb+"a menaquinol"Thumb+Thumb+"a menaquinone"
Nitrate + 2 Hydrogen ion + "a menaquinol" → Nitrite + Water + "a menaquinone"
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Acceptor+Thumb+AcceptorThumb+Reduced acceptor+Reduced acceptor
Nitrite + Acceptor + Water + Acceptor ↔ Nitrate + Reduced acceptor + Reduced acceptor
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2 Ferricytochrome c+Thumb+ThumbThumb+2 Ferrocytochrome c+2 Thumb
2 Ferricytochrome c + Nitrite + WaterNitrate + 2 Ferrocytochrome c + 2 Hydrogen ion
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Thumb+acceptorThumb+reduced acceptor
Nitrite + acceptor → Nitrate + reduced acceptor
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Nitrate+cytochrome c nitrite reductase+ThumbNitrite+cytochrome c nitrite reductase+Thumb+Thumb
Nitrate + cytochrome c nitrite reductase + Nitrate ↔ Nitrite + cytochrome c nitrite reductase + Water + Nitrite
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Thumb+2 Thumb+2 ElectronThumb+Thumb
Thumb
nitrite + an oxidized unknown electron acceptor + H2O = nitrate + an reduced unknown electron acceptor
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Menaquinones+PAMDB001633Menaquinols
Menaquinones + Hydrogen ion → Menaquinols
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PAMDB000468+PAMDB001633PAMDB000465+PAMDB000142
PAMDB000468PAMDB000465
PAMDB000468+UbiquinolsPAMDB000465+PAMDB000142+Ubiquinones
Nitrate + Ubiquinols → Nitrite + Water + Ubiquinones
ReactionCard
PAMDB000468+PAMDB001633+MenaquinolsPAMDB000465+PAMDB000142+Menaquinones+PAMDB001633
Nitrate + Hydrogen ion + Menaquinols → Nitrite + Water + Menaquinones + Hydrogen ion
ReactionCard
Complex Reactions:
2 Thumb+Thumb+ThumbThumb+Thumb+Thumb+2 Thumb
2 Thumb+Thumb+ThumbThumb+Menaquinone 8+Thumb+2 Thumb
Thumb+acceptorThumb+reduced acceptor
Nitrite + acceptor → Nitrate + reduced acceptor
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB002046Ferricytochrome cMetaboCard
PAMDB003525Ferrocytochrome cMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001653Menaquinol 8MetaboCard
PAMDB000468NitrateMetaboCard
PAMDB000465NitriteMetaboCard
PAMDB004318SelenateMetaboCard
PAMDB001752SeleniteMetaboCard
PAMDB000237Ubiquinol-8MetaboCard
PAMDB001700Ubiquinone-8MetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Component
macromolecular complex
nitrate reductase complex
protein complex
Function
catalytic activity
nitrate reductase activity
oxidoreductase activity
oxidoreductase activity, acting on other nitrogenous compounds as donors
Process
metabolic process
nitrate metabolic process
nitrogen compound metabolic process
oxidation reduction
Gene Properties
Locus tag: PA3874
Strand: -
Entrez Gene ID: 879783
Accession: NP_252563.1
GI: 15599069
Sequence start: 4336708
Sequence End: 4338249
Sequence Length: 1541
Gene Sequence:
>PA3874
ATGAAAATTCGTTCGCAAGTCGGCATGGTGCTGAACCTCGACAAATGCATCGGTTGCCACACCTGCTCGATCACCTGCAAGAACGTCTGGACCAGCCGCGAAGGCATGGAGTACGCCTGGTTCAACAACGTCGAGACCAAGCCCGGGATCGGCTACCCGAAGGAATGGGAAAACCAGGAGAAGTGGAAGGGCGGCTGGGTGCGCGCGGCAGACGGTTCGATCCGCCCGCGCATCGGCGGCAAGTTCCGCGTGCTGGCGAACATCTTCGCCAACCCGGACCTGCCCGAGATCGACGACTACTACGAACCGTTCGACTTCGACTACCAGCACCTGCATACCGCGCCCAAGGCCGAGCACCAGCCGGTGGCGCGCCCGCGCTCGCTGGTCTCCGGGCAGCGCATGGAGAAGATCGAGTGGGGCCCGAACTGGGAGGAGATCCTCGGCACCGAGTTCGCCAAGCGGCGCAAGGACAAGAACTTCGACCAGGTCCAGGCGGACATCTACGGTGAGTACGAGAACACCTTCATGATGTACCTGCCGCGCCTCTGCGAGCACTGCCTGAACCCGGCGTGCGTGGCGTCCTGCCCGAGCGGGGCGATCTACAAGCGCGAGGAGGACGGCATCGTCCTGATCGACCAGGACAAGTGCCGCGGCTGGCGGATGTGCATCTCCGGCTGCCCGTACAAGAAGATCTACTTCAACTGGAAGAGCGGCAAGTCCGAGAAGTGCATCTTCTGCTACCCGCGCATCGAGGCCGGCCAGCCCACCGTCTGCTCGGAGACCTGCGTCGGGCGCATCCGCTACCTCGGCGTGCTGCTCTACGACGCCGACCGTATCCACGAAGTGGCCAGTTGCGAGAACGAGCGCGAGCTGTACGAGAAGCAACTGGAGATCTTCCTCGATCCGTTCGACCCGGCGGTGATCGCCCAGGCGCGCAAGGATGGGGTGGCCGACAGCGTCATCGAGGCGGCGCAGAAGTCGCCGGTGTACAAGCTGGCGATGGACTGGAAGCTGGCCCTGCCGCTGCACCCGGAATACCGCACGCTGCCGATGGTCTGGTACGTGCCGCCGCTGTCGCCGATCCAGAACGCCGCCGCCGAGGGGCACATCGGCAGCGACGGGGTGATCCCGGACGTCGAGTCGCTGCGCATCCCCGTGCAGTACCTGGCCAACCTGCTCACCGCCGGCGACACCGCGCCGGTGCTGCTGGCGCTCAAGCGCCTGCTGGCGATGCGCGCCTACAAGCGCGCCGAGCACGTCGAAGGCCGCCAGGACCTGGAGGTGCTGGCCAAGGTCGGGTTGAGCGTGGAGCAGGTGGAGGAGATGTACCGCTACCTGGCCATCGCCAACTACGAGGATCGCTTCGTGATCCCCAGCGCGCACCGCGAGGAAGCGCTTTCCGATGCCTTCGCCGAGCGTTCCGGCTGTGGCTTCAGCTTCGGCAACGGCTGTTCCGGCGGCAGCAACTCCGCCGTCAACCTGTTCGGCGGCAAGCCGACCAACCGCCGCGACGTGATCCAGGTCGTGCAGATCCAGGAGTGA
Protein Properties
Protein Residues: 513
Protein Molecular Weight: 58.1 kDa
Protein Theoretical pI: 6.05
Hydropathicity (GRAVY score): -0.366
Charge at pH 7 (predicted): -6.12
Protein Sequence:
>PA3874
MKIRSQVGMVLNLDKCIGCHTCSITCKNVWTSREGMEYAWFNNVETKPGIGYPKEWENQEKWKGGWVRAADGSIRPRIGGKFRVLANIFANPDLPEIDDYYEPFDFDYQHLHTAPKAEHQPVARPRSLVSGQRMEKIEWGPNWEEILGTEFAKRRKDKNFDQVQADIYGEYENTFMMYLPRLCEHCLNPACVASCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIHEVASCENERELYEKQLEIFLDPFDPAVIAQARKDGVADSVIEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSPIQNAAAEGHIGSDGVIPDVESLRIPVQYLANLLTAGDTAPVLLALKRLLAMRAYKRAEHVEGRQDLEVLAKVGLSVEQVEEMYRYLAIANYEDRFVIPSAHREEALSDAFAERSGCGFSFGNGCSGGSNSAVNLFGGKPTNRRDVIQVVQIQE
References
External Links:
Resource Link
Genome ID: PA3874
Entrez Gene ID: 879783
NCBI Protein ID: 15599069
General Reference: PaperBLAST - Find papers about PA3874 and its homologs