Bifunctional protein glmU (PA5552)

Identification
Name: Bifunctional protein glmU
Synonyms:
  • UDP-N-acetylglucosamine pyrophosphorylase
  • N-acetylglucosamine-1-phosphate uridyltransferase
  • Glucosamine-1-phosphate N-acetyltransferase
Gene Name: glmU
Enzyme Class:
Biological Properties
General Function: Involved in magnesium ion binding
Specific Function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb
Uridine triphosphate + Glucosamine-1P ↔ Pyrophosphate + Uridine diphosphate-N-acetylglucosamine
ReactionCard
Thumb+alpha-D-Glucosamine 1-phosphate ↔ Thumb+Thumb
Acetyl-CoA + alpha-D-Glucosamine 1-phosphate ↔ Coenzyme A + Glucosamine-1P
ReactionCard
SMPDB Reactions:
Glucosamine-1P+Thumb+Thumb → N-Acetyl-glucosamine 1-phosphate+Thumb+Thumb+Thumb
Glucosamine-1P + Acetyl-CoA + Glucosamine-1P → N-Acetyl-glucosamine 1-phosphate + Coenzyme A + Hydrogen ion + N-Acetyl-glucosamine 1-phosphate
ReactionCard
N-Acetyl-glucosamine 1-phosphate+Uridine triphosphate+Thumb+Thumb+Thumb → Thumb+Pyrophosphate
N-Acetyl-glucosamine 1-phosphate + Uridine triphosphate + Hydrogen ion + N-Acetyl-glucosamine 1-phosphate + Uridine triphosphate → Uridine diphosphate-N-acetylglucosamine + Pyrophosphate
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb
N-Acetyl-glucosamine 1-phosphate + Uridine triphosphate + Hydrogen ion → Uridine diphosphate-N-acetylglucosamine + Pyrophosphate
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
Acetyl-CoA + N-Acetyl-glucosamine 1-phosphate → N-Acetyl-glucosamine 1-phosphate + Coenzyme A + Hydrogen ion
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb
N-Acetyl-glucosamine 1-phosphate + Hydrogen ion + Uridine triphosphate → Pyrophosphate + Uridine diphosphate-N-acetylglucosamine
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
Uridine triphosphate + Glucosamine-1P ↔ Pyrophosphate + Uridine diphosphate-N-acetylglucosamine
ReactionCard
Thumb+alpha-D-Glucosamine 1-phosphate ↔ Thumb+Thumb
Acetyl-CoA + alpha-D-Glucosamine 1-phosphate ↔ Coenzyme A + Glucosamine-1P
ReactionCard
Glucosamine-1P+Thumb+Thumb → N-Acetyl-glucosamine 1-phosphate+Thumb+Thumb+Thumb
Glucosamine-1P + Acetyl-CoA + Glucosamine-1P → N-Acetyl-glucosamine 1-phosphate + Coenzyme A + Hydrogen ion + N-Acetyl-glucosamine 1-phosphate
ReactionCard
N-Acetyl-glucosamine 1-phosphate+Uridine triphosphate+Thumb+Thumb+Thumb → Thumb+Pyrophosphate
N-Acetyl-glucosamine 1-phosphate + Uridine triphosphate + Hydrogen ion + N-Acetyl-glucosamine 1-phosphate + Uridine triphosphate → Uridine diphosphate-N-acetylglucosamine + Pyrophosphate
ReactionCard
Thumb
D-glucosamine 1-phosphate + acetyl-CoA = N-acetyl-α-D-glucosamine 1-phosphate + coenzyme A
ReactionCard
Thumb
N-acetyl-α-D-glucosamine 1-phosphate + UTP + H+ → UDP-N-acetyl-α-D-glucosamine + diphosphate
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Complex Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
Acetyl-CoA + N-Acetyl-glucosamine 1-phosphate → N-Acetyl-glucosamine 1-phosphate + Coenzyme A + Hydrogen ion
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb
N-Acetyl-glucosamine 1-phosphate + Hydrogen ion + Uridine triphosphate → Pyrophosphate + Uridine diphosphate-N-acetylglucosamine
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000298Acetyl-CoAMetaboCard
PAMDB002052alpha-D-Glucosamine 1-phosphateMetaboCard
PAMDB002053beta-D-Fructose 2-phosphateMetaboCard
PAMDB000381Coenzyme AMetaboCard
PAMDB000256Glucosamine-1PMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000357N-Acetyl-glucosamine 1-phosphateMetaboCard
PAMDB000633PyrophosphateMetaboCard
PAMDB000120Uridine diphosphate-N-acetylglucosamineMetaboCard
PAMDB000117Uridine triphosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
acetyltransferase activity
acyltransferase activity
binding
catalytic activity
cation binding
glucosamine-1-phosphate N-acetyltransferase activity
ion binding
magnesium ion binding
metal ion binding
N-acetyltransferase activity
nucleotidyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
transferase activity, transferring phosphorus-containing groups
UDP-N-acetylglucosamine diphosphorylase activity
uridylyltransferase activity
Process
aminoglycan metabolic process
anatomical structure morphogenesis
biosynthetic process
carbohydrate metabolic process
cell morphogenesis
cellular component morphogenesis
developmental process
glycosaminoglycan metabolic process
lipid biosynthetic process
lipid metabolic process
lipopolysaccharide biosynthetic process
metabolic process
peptidoglycan biosynthetic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Locus tag: PA5552
Strand: -
Entrez Gene ID: 877892
Accession: NP_254239.1
GI: 15600745
Sequence start: 6246326
Sequence End: 6247690
Sequence Length: 1364
Gene Sequence: 
>PA5552
ATGTCCCTTGAAATCGTGATTCTCGCCGCCGGCCAGGGCACCCGCATGCGTTCGGCCCTGCCCAAGGTCCTCCATCCCATTGCCGGCAAGCCGATGCTCGGCCACGTCATCGACTGCGCGCGCCAGTTGCAGCCGGAACGGATCCACGTGGTGATCGGCCACGGCGCGGATCTGGTGCGTGAGCGGATGGCCGCCGACGACCTGAATTTCGTGCTCCAGGCCGAACAGCTCGGTACGGGCCATGCGGTGGCCCAGGCCCTGCCGTTCCTCAGTGCAGACCAGGTGCTGATCCTCTACGGCGACGTGCCGCTGATCCAGCTGGACACCTTGCAGCGCCTGCTGGCGCAAGTCACCCCGGACCAGTTGTCGTTGCTCACCGTCGACATGCTCGACCCCACCGGCTACGGCCGCATCGTCCGTGACGACCAGGGCGCGGTGCAGGCCATCGTCGAGCACAAGGACGCGACCCCGGCGCAGCGCCAGATCGGCGAGATCAACACCGGCATCCTCGCGGTGCCGGGCAAGCGCCTTGCCGACTGGCTCGGCCGCCTGTCCAACGACAACGCCCAGGGCGAGTACTACCTGACCGACGTGATCGCCATGGCGGTCGGCGACGGCCTGGTGGTGGCCAGCGCCCAACCGCTGGACGCGATGGAAGTGCAGGGCGTCAATGACCGCATGCAACAGGCCCAGCTGGAGCGTCACTACCAGCGCCTGCGCGCAGAGGAGCTGATGCGCCAGGGCGTGACCCTGCTCGATCCGCAGCGCCTGGATGTCCGTGGCGAAATCTCGGTCGGCCGCGACGTGCTGATCGACGTCAATGTGGTGCTGGAAGGCCGCGTGGTGATCGAGGACGACGTGCGCATCGGACCGAACTGCGTGATTCGCGACAGCGTCCTGCGGCGTGGCGCGGTGATCAAGGCCAACAGCCACCTGGAAGGCGCGGAGCTGGGCGAGGGTAGCGATGCCGGTCCGTTCGCCCGCCTGCGTCCGGGCAGTGTGCTCGGCGCTCGGGCCCATGTCGGCAACTTCGTCGAACTGAAGAATGCCCGCCTGGGCGAGGGGAGCAAGGCCGGCCACCTGAGCTACCTGGGCGATGCCGAGCTGGGCGCCAACTGCAACATCGGCGCCGGCACCATCACCTGCAACTACGATGGCGCGAACAAGTTCCGCACCGAGCTGGGCGATGATGTGTTCATCGGCTCGAACAACTCGCTGGTGGCGCCGCTGAAGATCGGCGATGGCGCGACGACCGCGGCCGGCTCCACCATCACCCATGAGGTTCCGGCGAAGAACCTGGCCTTCGGCCGCGCCCGGCAGAAGAACCTGGAAAACTGGAAGCGCCCGGAAAAAATCAAGAAGTAA
Protein Properties
Protein Residues: 454
Protein Molecular Weight: 48.9 kDa
Protein Theoretical pI: 6.15
Hydropathicity (GRAVY score): -0.114
Charge at pH 7 (predicted): -5.46
Protein Sequence: 
>PA5552
MSLEIVILAAGQGTRMRSALPKVLHPIAGKPMLGHVIDCARQLQPERIHVVIGHGADLVRERMAADDLNFVLQAEQLGTGHAVAQALPFLSADQVLILYGDVPLIQLDTLQRLLAQVTPDQLSLLTVDMLDPTGYGRIVRDDQGAVQAIVEHKDATPAQRQIGEINTGILAVPGKRLADWLGRLSNDNAQGEYYLTDVIAMAVGDGLVVASAQPLDAMEVQGVNDRMQQAQLERHYQRLRAEELMRQGVTLLDPQRLDVRGEISVGRDVLIDVNVVLEGRVVIEDDVRIGPNCVIRDSVLRRGAVIKANSHLEGAELGEGSDAGPFARLRPGSVLGARAHVGNFVELKNARLGEGSKAGHLSYLGDAELGANCNIGAGTITCNYDGANKFRTELGDDVFIGSNNSLVAPLKIGDGATTAAGSTITHEVPAKNLAFGRARQKNLENWKRPEKIKK
References
External Links:
Resource Link
Genome ID: PA5552
Entrez Gene ID: 877892
NCBI Protein ID: 15600745
General Reference: PaperBLAST - Find papers about PA5552 and its homologs