tRNA sulfurtransferase (PA5118)

Identification
Name: tRNA sulfurtransferase
Synonyms:
  • Sulfur carrier protein ThiS sulfurtransferase
  • Thiamine biosynthesis protein thiI
  • tRNA 4-thiouridine synthase
Gene Name: thiI
Enzyme Class:
Biological Properties
General Function: Involved in RNA binding
Specific Function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by iscS
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
[Enzyme]-S-sulfanylcysteine+C15813 ↔ C15814
[Enzyme]-S-sulfanylcysteine + C15813 ↔ C15814
ReactionCard
Thumb+'Activated' tRNA ↔ Thumb+tRNA containing a thionucleotide
L-Cysteine + 'Activated' tRNA ↔ L-Serine + tRNA containing a thionucleotide
ReactionCard
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+'activated' tRNA → Thumb+tRNA containing a thionucleotide
L-Cysteine + 'activated' tRNA → L-Serine + tRNA containing a thionucleotide
ReactionCard
[IscS]-SSH+[ThiS]-COAMP → [IscS]-SH+[ThiS]-COSH+Thumb
[IscS]-SSH + [ThiS]-COAMP → [IscS]-SH + [ThiS]-COSH + Adenosine monophosphate
ReactionCard
Complex Reactions:
Thumb+Thumb+Thumb+Thumb+IscS with bound sulfur+Thumb → Thumb+Thumb+Thumb+2 Thumb+IscS sulfur acceptor protein+Thumb+Thumb
Adenosine triphosphate + Dehydroglycine + 1-Deoxy-D-xylulose 5-phosphate + Hydrogen ion + IscS with bound sulfur + NADPH → 4-Methyl-5-(2-phosphoethyl)-thiazole + Adenosine monophosphate + Carbon dioxide + 2 Water + IscS sulfur acceptor protein + NADP + Pyrophosphate
ReactionCard
Thumb+'activated' tRNA → Thumb+tRNA containing a thionucleotide
L-Cysteine + 'activated' tRNA → L-Serine + tRNA containing a thionucleotide
ReactionCard
[IscS]-SSH+[ThiS]-COAMP → [IscS]-SH+[ThiS]-COSH+Thumb
[IscS]-SSH + [ThiS]-COAMP → [IscS]-SH + [ThiS]-COSH + Adenosine monophosphate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0003011-Deoxy-D-xylulose 5-phosphateMetaboCard
PAMDB0009424-Methyl-5-(2-phosphoethyl)-thiazoleMetaboCard
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000568Carbon dioxideMetaboCard
PAMDB001730DehydroglycineMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000152L-CysteineMetaboCard
PAMDB000077L-SerineMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
PAMDB000633PyrophosphateMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
binding
nucleic acid binding
RNA binding
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
thiamin and derivative metabolic process
thiamin biosynthetic process
thiamin metabolic process
Gene Properties
Locus tag: PA5118
Strand: -
Entrez Gene ID: 877687
Accession: NP_253805.1
GI: 15600311
Sequence start: 5764693
Sequence End: 5766147
Sequence Length: 1454
Gene Sequence: 
>PA5118
ATGAAACTCATCGTCAAGACCTTCCAGGAAATCACCATCAAGAGCCGGCCGGTGCGCAAGCGCTTCATCCGGCAGTTGGCGAAGAACATCCGCGCGGTGCTGCGCGACCTGGACCCGGAGCTGAAGGTCGAGGGCGAGTGGGACAACCTGGAGGTGGAGACCGCCGTGGTCGACGCCAAGGCCAGGCGCGAGATGATCGAGCGCCTGACCTGCACCCCGGGCATCGGTCATTTCCTCGAGGTCCACGAGTATCCGCTGGGCGATTTCGACGACATCCTCGCCAAGTGCAAGGCGCATTTCGGCGACCAGTTGGCCGGCAAGACCTTCGCCGTGCGCTGCAAGCGCGCCGGCAAGCACGCGTTCACCTCGATGGAGGTGGAGCGTTACGTCGGCAGCGGCCTGCGCCGCGAATGCGGGGCTGCCGGGATCGACCTGAAGCAGCCGGAAGTCGAGGTGCGGATGGAAATCCGCCTCGACCGCCTGTTCGTCATCCATCGCCAGCATCCGGGCCTGGGCGGCTATCCGCTGGGCGCGCTGGAACAGGTGCTGGTGCTGATGTCCGGCGGCTTCGACTCGACCGTGGCGGCCTACCAGATGATGCGCCGCGGGATGATCAGCCACTTCGTGTTCTTCAACCTCGGCGGGCGCGCCCACGAACTGGGGGTGATGGAAGTCGCCCACTACCTGTGGGAGAAGTACGGCCGCTCGCAGCGCGTGCTGTTCGTCAGCGTGCCGTTCGAGGAAGTGGTCGGCGAGATCCTCACCAAGGTCGACGACAGCTATATGGGCGTGACCCTCAAGCGCATGATGCTGCGCGCCGCCAGCCGCGTGGCCGAGCGCCTGGAGCTGGACGCGCTGGTGACCGGCGAGGCGATCTCCCAGGTGTCCAGCCAGACCCTGCCGAACCTCTCGGTGATCGACCGGGTTACCGACACCCTGGTGCTGCGCCCGCTGATCGTCAGCCACAAGCAGGACATCATCGACACCGCCCGGCAGATCGGCACCGCCGAGTTCGCCCGGCACATGCCGGAATACTGCGGGGTGATCTCGGTGAACCCGACCACCCAGGCCAAGCCCTACCGCGTCGAGCACGAAGAGTCGAAATTCGACATGGCGGTGCTCGAGCGCGCCCTGGAGCGAGCCACCCAGCGCACCGTCGACCGGGTGATCGACGAACTCGGCCAGGACCTGCAGGTGGAAGAGGTCGGCGAGGTGCTGCCCGGTCAGATTGTCATCGATATCCGCCATCCTGATGCCCAGGAAGACGAACCCCTGGCCCTGGAAGGCGTCGAAGTCCAGGCGCTGCCGTTCTACGCGATCAACAGCCGCTTCAAGGAACTGGACGCCAACCGCCAGTACCTCCTGTATTGCGACAAAGGGGTGATGAGCCGCCTGCATGCCCATCACCTGCTCAACGAGGGGCACACCAATGTGCGTGTTTATCGTCCGGCTTAA
Protein Properties
Protein Residues: 484
Protein Molecular Weight: 54.8 kDa
Protein Theoretical pI: 6.66
Hydropathicity (GRAVY score): -0.201
Charge at pH 7 (predicted): -3.06
Protein Sequence: 
>PA5118
MKLIVKTFQEITIKSRPVRKRFIRQLAKNIRAVLRDLDPELKVEGEWDNLEVETAVVDAKARREMIERLTCTPGIGHFLEVHEYPLGDFDDILAKCKAHFGDQLAGKTFAVRCKRAGKHAFTSMEVERYVGSGLRRECGAAGIDLKQPEVEVRMEIRLDRLFVIHRQHPGLGGYPLGALEQVLVLMSGGFDSTVAAYQMMRRGMISHFVFFNLGGRAHELGVMEVAHYLWEKYGRSQRVLFVSVPFEEVVGEILTKVDDSYMGVTLKRMMLRAASRVAERLELDALVTGEAISQVSSQTLPNLSVIDRVTDTLVLRPLIVSHKQDIIDTARQIGTAEFARHMPEYCGVISVNPTTQAKPYRVEHEESKFDMAVLERALERATQRTVDRVIDELGQDLQVEEVGEVLPGQIVIDIRHPDAQEDEPLALEGVEVQALPFYAINSRFKELDANRQYLLYCDKGVMSRLHAHHLLNEGHTNVRVYRPA
References
External Links:
Resource Link
Genome ID: PA5118
Entrez Gene ID: 877687
NCBI Protein ID: 15600311
General Reference: PaperBLAST - Find papers about PA5118 and its homologs