Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (PA5016)

Identification
Name: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
Synonyms:
  • Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
  • E2
Gene Name: aceF
Enzyme Class:
Biological Properties
General Function: Involved in transferase activity, transferring acyl groups
Specific Function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3)
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Enzyme N6-(dihydrolipoyl)lysine+Thumb ↔ Thumb+[Dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine
Acetyl-CoA + Enzyme N6-(dihydrolipoyl)lysine + Enzyme N6-(dihydrolipoyl)lysine ↔ Coenzyme A + [Dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine
ReactionCard
SMPDB Reactions:
Thumb+Dihydrolipoamide+Thumb ↔ Thumb+Thumb
Acetyl-CoA + Dihydrolipoamide + Dihydrolipoamide ↔ Coenzyme A + S-Acetyldihydrolipoamide
ReactionCard
Succinyl-CoA+Dihydrolipoamide+Thumb+Thumb → Thumb+Thumb
Succinyl-CoA + Dihydrolipoamide + Succinyl-CoA + Dihydrolipoamide → Coenzyme A + (S)-Succinyldihydrolipoamide
ReactionCard
a [pyruvate dehydrogenase E2 protein] N6-S-acetyldihydrolipoyl-L-lysine+Thumb → a [pyruvate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine+Thumb
a [pyruvate dehydrogenase E2 protein] N6-S-acetyldihydrolipoyl-L-lysine + Coenzyme A → a [pyruvate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + Acetyl-CoA
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
Coenzyme A + NAD + Pyruvic acid → Acetyl-CoA + Carbon dioxide + NADH
ReactionCard
Complex Reactions: Not Available
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB004121(S)-SuccinyldihydrolipoamideMetaboCard
PAMDB000298Acetyl-CoAMetaboCard
PAMDB000568Carbon dioxideMetaboCard
PAMDB000381Coenzyme AMetaboCard
PAMDB000215DihydrolipoamideMetaboCard
PAMDB004301Enzyme N6-(dihydrolipoyl)lysineMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000102Pyruvic acidMetaboCard
PAMDB000411S-AcetyldihydrolipoamideMetaboCard
PAMDB000228Succinyl-CoAMetaboCard
GO Classification:
Component
macromolecular complex
protein complex
pyruvate dehydrogenase complex
Function
acetyltransferase activity
acyltransferase activity
binding
catalytic activity
dihydrolipoyllysine-residue acetyltransferase activity
protein binding
S-acetyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
alcohol metabolic process
glucose catabolic process
glucose metabolic process
glycolysis
hexose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
Gene Properties
Locus tag: PA5016
Strand: +
Entrez Gene ID: 881297
Accession: NP_253703.1
GI: 15600209
Sequence start: 5638949
Sequence End: 5640592
Sequence Length: 1643
Gene Sequence: 
>PA5016
GTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAGCTGCTGGTCAAGCCCGGCGACAAGGTCGAGGCCGATCAGAGCCTGCTGACCCTGGAATCCGACAAGGCCAGCATGGAAATCCCCAGTCCCAAGGCCGGGGTAGTGAAAAGCATCAAGGCGAAGGTCGGCGACACCTTGAAAGAAGGTGACGAAATCCTCGAGCTGGAAGTGGAAGGCGGCGAACAGCCTGCCGAAGCCAAGGCCGAGGCAGCGCCCGCCCAACCGGAAGCGCCGAAAGCCGAAGCGCCTGCTCCCGCCCCGAGCGAGAGCAAGCCGGCCGCCCCCGCCGCGGCCAGCGTCCAGGACATCAAGGTCCCGGACATCGGCTCGGCCGGCAAGGCCAACGTCATCGAAGTGATGGTCAAGGCCGGCGACACGGTCGAGGCCGACCAGTCGCTGATCACCCTGGAATCCGACAAGGCCAGCATGGAGATCCCCTCGCCGGCCTCCGGGGTGGTGGAAAGCGTCTCGATCAAGGTCGGTGACGAAGTCGGCACCGGCGACCTGATCCTCAAGCTGAAGGTGGAAGGCGCCGCTCCGGCAGCCGAAGAGCAACCGGCAGCCGCTCCGGCCCAGGCCGCGGCGCCCGCCGCCGAGCAGAAGCCCGCCGCGGCGGCCCCTGCGCCAGCCAAGGCCGATACCCCGGCTCCGGTCGGCGCACCCAGCCGCGACGGCGCCAAGGTCCACGCCGGCCCGGCGGTGCGCATGCTGGCGCGCGAGTTCGGCGTCGAGCTGAGCGAAGTGAAAGCCAGCGGTCCCAAGGGTCGCATCCTCAAGGAAGACGTCCAGGTCTTCGTCAAGGAGCAACTGCAGCGCGCCAAGTCCGGCGGTGCCGGCGCCACCGGCGGAGCCGGCATCCCGCCGATCCCGGAAGTCGACTTCAGCAAGTTCGGCGAAGTGGAAGAAGTGGCGATGACCCGCCTGATGCAGGTCGGCGCCGCCAACCTGCATCGCAGCTGGCTGAACGTGCCGCACGTGACCCAGTTCGACCAGTCGGACATCACCGACATGGAAGCCTTCCGCGTTGCCCAGAAGGCCGCGGCGGAGAAGGCCGGGGTCAAGCTGACCGTACTGCCGATCCTGCTCAAGGCCTGCGCCCACCTGCTCAAGGAACTGCCGGACTTCAACAGTTCGCTGGCCCCCAGCGGCAAGGCGCTGATCCGCAAGAAGTACGTACACATCGGCTTCGCCGTGGACACTCCGGACGGCCTGCTGGTCCCGGTGATCCGCGATGTCGACCGGAAGAGCCTCCTGCAACTGGCCGCCGAGGCCGCCGACCTGGCCGACAAGGCCCGCAACAAGAAGCTCTCGGCCGATGCCATGCAGGGCGCCTGCTTCACCATCTCCAGTCTCGGCCACATCGGCGGCACCGGCTTCACGCCGATCGTCAACGCGCCGGAAGTGGCGATCCTCGGTGTGTCCAAGGCGACCATGCAGCCGGTATGGGACGGCAAGGCCTTCCAGCCGCGCCTGATGCTGCCGCTGTCGCTGTCCTACGACCATCGCGTGATCAACGGTGCCGCCGCGGCGCGCTTCACCAAGCGCCTGGGCGAGCTGCTGGCGGACATCCGCACCCTGCTCCTGTAA
Protein Properties
Protein Residues: 547
Protein Molecular Weight: 56.7 kDa
Protein Theoretical pI: 4.99
Hydropathicity (GRAVY score): -0.041
Charge at pH 7 (predicted): -12.33
Protein Sequence: 
>PA5016
MSELIRVPDIGNGEGEVIELLVKPGDKVEADQSLLTLESDKASMEIPSPKAGVVKSIKAKVGDTLKEGDEILELEVEGGEQPAEAKAEAAPAQPEAPKAEAPAPAPSESKPAAPAAASVQDIKVPDIGSAGKANVIEVMVKAGDTVEADQSLITLESDKASMEIPSPASGVVESVSIKVGDEVGTGDLILKLKVEGAAPAAEEQPAAAPAQAAAPAAEQKPAAAAPAPAKADTPAPVGAPSRDGAKVHAGPAVRMLAREFGVELSEVKASGPKGRILKEDVQVFVKEQLQRAKSGGAGATGGAGIPPIPEVDFSKFGEVEEVAMTRLMQVGAANLHRSWLNVPHVTQFDQSDITDMEAFRVAQKAAAEKAGVKLTVLPILLKACAHLLKELPDFNSSLAPSGKALIRKKYVHIGFAVDTPDGLLVPVIRDVDRKSLLQLAAEAADLADKARNKKLSADAMQGACFTISSLGHIGGTGFTPIVNAPEVAILGVSKATMQPVWDGKAFQPRLMLPLSLSYDHRVINGAAAARFTKRLGELLADIRTLLL
References
External Links:
Resource Link
Genome ID: PA5016
Entrez Gene ID: 881297
NCBI Protein ID: 15600209
General Reference: PaperBLAST - Find papers about PA5016 and its homologs