UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (PA4416)

Identification
Name: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
Synonyms:
  • D-alanyl-D-alanine-adding enzyme
  • UDP-MurNAc-pentapeptide synthetase
Gene Name: murF
Enzyme Class:
Biological Properties
General Function: Involved in ATP binding
Specific Function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
D-Alanyl-D-alanine + Adenosine triphosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminoheptanedioate → ADP + Hydrogen ion + Phosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Adenosine triphosphate + UDP-N-Acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-Alanyl-D-alanine ↔ ADP + Phosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Adenosine triphosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminoheptanedioate + D-Alanyl-D-alanine ↔ ADP + Phosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine
ReactionCard
SMPDB Reactions:
Thumb+Thumb+Thumb → Adenosine diphosphate+Thumb+Thumb+Thumb+Thumb
UDP-N-Acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminopimelate + D-Alanyl-D-alanine + Adenosine triphosphate → Adenosine diphosphate + Phosphate + Hydrogen ion + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + ADP
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
UDP-N-Acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminopimelate + D-Alanyl-D-alanine + Adenosine triphosphate → N-Acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine + ADP + Phosphate + Hydrogen ion
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
D-Alanyl-D-alanine + Adenosine triphosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminoheptanedioate → ADP + Hydrogen ion + Phosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
D-Alanyl-D-alanine + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminoheptanedioate + Adenosine triphosphate → Hydrogen ion + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine + Phosphate + ADP
ReactionCard
Complex Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
Adenosine triphosphate + UDP-N-Acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-Alanyl-D-alanine → ADP + Inorganic phosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000345ADPMetaboCard
PAMDB000504D-Alanyl-D-alanineMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001776Inorganic phosphateMetaboCard
PAMDB001129N-Acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamineMetaboCard
PAMDB000383PhosphateMetaboCard
PAMDB006368UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanineMetaboCard
PAMDB001049UDP-N-Acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminopimelateMetaboCard
UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanineMetaboCard
PAMDB001051UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanineMetaboCard
PAMDB000650UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminoheptanedioateMetaboCard
PAMDB001052UDP-N-Acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysineMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
acid-amino acid ligase activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
nucleoside binding
purine nucleoside binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
Process
aminoglycan metabolic process
biological regulation
biosynthetic process
carbohydrate metabolic process
cell division
cell wall biogenesis
cell wall organization or biogenesis
cellular cell wall organization or biogenesis
cellular component organization or biogenesis
cellular process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan biosynthetic process
peptidoglycan metabolic process
peptidoglycan-based cell wall biogenesis
polysaccharide metabolic process
primary metabolic process
regulation of biological quality
regulation of cell shape
Gene Properties
Locus tag: PA4416
Strand: -
Entrez Gene ID: 881289
Accession: NP_253106.1
GI: 15599612
Sequence start: 4949772
Sequence End: 4951148
Sequence Length: 1376
Gene Sequence: 
>PA4416
ATGCTTGAGCCTCTTCGCCTCAGCCAGTTGACGGTCGCGCTGGACGCCCGCCTGATCGGCGAGGACGCCGTCTTTTCGGCGGTTTCCACCGACAGTCGCGCCATCGGGCCCGGCCAACTGTTCATTGCCCTGAGTGGGCCGCGTTTCGACGGCCACGACTATCTCGCCGAGGTTGCCGCCAAGGGCGCGGTGGCTGCGCTGGTGGAGCGCGAAGTCGCCGACGCGCCCCTGCCGCAATTGCTGGTGCGCGATACCCGTGCGGCCCTGGGGCGACTGGGCGCGCTGAACCGGCGCAAGTTCACCGGCCCGCTGGCGGCCATGACGGGCTCCAGCGGCAAGACCACGGTCAAGGAGATGCTCGCCAGCATCCTGCGTACCCAGGCCGGCGATGCCGAGTCGGTGCTGGCTACCCGTGGCAATCTGAACAACGACCTCGGCGTACCGCTGACCCTGCTGCAACTGGCGCCGCAGCACCGTAGCGCAGTGATCGAACTGGGCGCCTCGCGCATCGGCGAGATCGCCTACACGGTCGAGCTGACCCGCCCGCACGTGGCGATCATCACCAATGCCGGAACCGCCCATGTCGGCGAGTTCGGCGGACCGGAGAAGATCGTCGAGGCGAAGGGCGAGATACTCGAAGGGCTGGCCGCCGACGGCACCGCCGTACTGAACCTGGACGACAAGGCCTTCGACACCTGGAAGGCCCGTGCCAGCGGCCGTCCGTTGCTGACTTTCTCCCTCGACCGGCCCCAGGCCGATTTCCGCGCCGCCGATCTGCAGCGCGATGCGCGCGGCTGCATGGGCTTCAGGCTGCAGGGCGTAGCGGGTGAAGCGCAGGTCCAGCTCAACCTGCTGGGGCGGCACAATGTCGCCAATGCCCTGGCTGCCGCCGCTGCCGCCCATGCACTGGGCGTGCCGCTGGATGGGATCGTCGCCGGGCTGCAGGCGCTGCAGCCGGTCAAGGGCCGCGCGGTAGCGCAACTGACCGCCAGCGGGCTGCGTGTGATAGACGACAGCTACAACGCCAACCCCGCGTCAATGCTGGCGGCGATTGATATACTGAGCGGCTTTTCCGGGCGCACCGTCCTGGTCCTCGGAGACATGGGCGAACTCGGTTCCTGGGCCGAGCAGGCCCACCGCGAGGTGGGCGCCTACGCCGCTGGCAAGGTGTCCGCGCTCTATGCGGTCGGACCGCTGATGGCCCACGCCGTACAGGCGTTCGGCGCCACGGGCCGGCACTTCGCCGACCAGGCCAGCCTGATCGGGGCGCTGGCCACCGAACAACCGACAACCACCATTTTGATCAAGGGTTCCCGCAGTGCGGCGATGGACAAAGTCGTCGCGGCGCTGTGCGGTTCCTCCGAGGAGAGTCACTAA
Protein Properties
Protein Residues: 458
Protein Molecular Weight: 47.4 kDa
Protein Theoretical pI: 6.52
Hydropathicity (GRAVY score): 0.162
Charge at pH 7 (predicted): -2.64
Protein Sequence: 
>PA4416
MLEPLRLSQLTVALDARLIGEDAVFSAVSTDSRAIGPGQLFIALSGPRFDGHDYLAEVAAKGAVAALVEREVADAPLPQLLVRDTRAALGRLGALNRRKFTGPLAAMTGSSGKTTVKEMLASILRTQAGDAESVLATRGNLNNDLGVPLTLLQLAPQHRSAVIELGASRIGEIAYTVELTRPHVAIITNAGTAHVGEFGGPEKIVEAKGEILEGLAADGTAVLNLDDKAFDTWKARASGRPLLTFSLDRPQADFRAADLQRDARGCMGFRLQGVAGEAQVQLNLLGRHNVANALAAAAAAHALGVPLDGIVAGLQALQPVKGRAVAQLTASGLRVIDDSYNANPASMLAAIDILSGFSGRTVLVLGDMGELGSWAEQAHREVGAYAAGKVSALYAVGPLMAHAVQAFGATGRHFADQASLIGALATEQPTTTILIKGSRSAAMDKVVAALCGSSEESH
References
External Links:
Resource Link
Genome ID: PA4416
Entrez Gene ID: 881289
NCBI Protein ID: 15599612
General Reference: PaperBLAST - Find papers about PA4416 and its homologs