P. aeruginosa Metabolome Database Protein PA3814

Identification

Name:

Cysteine desulfurase

Synonyms:

NifS protein homolog
ThiI transpersulfidase

Gene Name:

iscS

Enzyme Class:

2.8.1.7

Biological Properties

General Function:

Involved in metabolic process

Specific Function:

Catalyzes the removal of elemental sulfur and selenium atoms from cysteine and selenocysteine to produce alanine. Functions as a sulfur delivery protein for NAD, biotin and Fe-S cluster synthesis. Transfers sulfur on 'Cys-456' of thiI in a transpersulfidation reaction. Transfers sulfur on 'Cys-19' of tusA in a transpersulfidation reaction. Functions also as a selenium delivery protein in the pathway for the biosynthesis of selenophosphate

Cellular Location:

Cytoplasm (Probable)

KEGG Pathways:

Metabolic pathways pae01100
Thiamine metabolism pae00730

KEGG Reactions:

[Enzyme]-cysteine

↔

[Enzyme]-S-sulfanylcysteine

[Enzyme]-cysteine + L-Cysteine ↔ [Enzyme]-S-sulfanylcysteine + L-Alanine
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SMPDB Reactions:

an [L-cysteine desulfurase] L-cysteine persulfide

→

an [L-cysteine desulfurase] L-cysteine persulfide

L-Alanine

L-Cysteine + an [L-cysteine desulfurase] L-cysteine persulfide → an [L-cysteine desulfurase] L-cysteine persulfide + L-Alanine + L-Alanine
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PseudoCyc/BioCyc Reactions:

IscS sulfur acceptor protein

→

IscS with bound sulfur

L-Cysteine + IscS sulfur acceptor protein → L-Alanine + IscS with bound sulfur
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[Enzyme]-cysteine

↔

[Enzyme]-S-sulfanylcysteine

[Enzyme]-cysteine + L-Cysteine ↔ [Enzyme]-S-sulfanylcysteine + L-Alanine
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acceptor

→

S-sulfanyl-acceptor

L-Cysteine + acceptor → L-Alanine + S-sulfanyl-acceptor
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an [L-cysteine desulfurase] L-cysteine persulfide

→

an [L-cysteine desulfurase] L-cysteine persulfide

L-Alanine

L-Cysteine + an [L-cysteine desulfurase] L-cysteine persulfide → an [L-cysteine desulfurase] L-cysteine persulfide + L-Alanine + L-Alanine
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3-sulfinoalanine + H₂O = L-alanine + sulfite + H⁺
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an S-sulfanyl-[L-cysteine desulfurase] + an unsulfurated [sulfur carrier] → L-cysteine desulfurase + a sulfurated [sulfur carrier]
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→

L-Cysteine + Hydrogen ion → L-Alanine
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an S-sulfanyl-[cysteine desulfurase]-[disordered-form scaffold protein] complex → a [cysteine desulfurase]-S-sulfanyl-[disordered-form scaffold protein] complex
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→

L-Cysteine → L-Alanine
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an S-sulfanyl-[cysteine desulfurase]-S-sulfanyl-[disordered-form scaffold protein] complex → a [cysteine desulfurase]-(S-sulfanyl)₂-[disordered-form scaffold protein] complex
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Complex Reactions:

IscS with bound sulfur

MoaD Protein with bound AMP

→

IscS sulfur acceptor protein

MoaD Protein with thiocarboxylate

IscS with bound sulfur + MoaD Protein with bound AMP + NADH → Adenosine monophosphate + IscS sulfur acceptor protein + MoaD Protein with thiocarboxylate + NAD
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[2Fe-1S] desulfurated iron-sulfur cluster

IscS with bound sulfur

IscU scaffold protein

→

IscS sulfur acceptor protein

IscU with bound [2Fe-2S] cluster

[2Fe-1S] desulfurated iron-sulfur cluster + IscS with bound sulfur + IscU scaffold protein → 4 Hydrogen ion + IscS sulfur acceptor protein + IscU with bound [2Fe-2S] cluster
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IscS with bound sulfur

→

IscS sulfur acceptor protein

Adenosine triphosphate + Dehydroglycine + 1-Deoxy-D-xylulose 5-phosphate + Hydrogen ion + IscS with bound sulfur + NADPH → 4-Methyl-5-(2-phosphoethyl)-thiazole + Adenosine monophosphate + Carbon dioxide + 2 Water + IscS sulfur acceptor protein + NADP + Pyrophosphate
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2 IscS with bound sulfur

IscU scaffold protein

→

2 IscS sulfur acceptor protein

IscU with bound [2Fe-2S] cluster

FADH2 + 2 Iron + 2 IscS with bound sulfur + IscU scaffold protein → FAD + 6 Hydrogen ion + 2 IscS sulfur acceptor protein + IscU with bound [2Fe-2S] cluster
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2 IscS with bound sulfur

IscU with bound [2Fe-2S] cluster

→

2 IscS sulfur acceptor protein

IscU with two bound [2Fe-2S] clusters

FADH2 + 2 Iron + 2 IscS with bound sulfur + IscU with bound [2Fe-2S] cluster → FAD + 6 Hydrogen ion + 2 IscS sulfur acceptor protein + IscU with two bound [2Fe-2S] clusters
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IscS sulfur acceptor protein

→

IscS with bound sulfur

L-Cysteine + IscS sulfur acceptor protein → L-Alanine + IscS with bound sulfur
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acceptor

→

S-sulfanyl-acceptor

L-Cysteine + acceptor → L-Alanine + S-sulfanyl-acceptor
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Transports:

Not Available

Metabolites:

PAMDB ID	Name	View
PAMDB000301	1-Deoxy-D-xylulose 5-phosphate	MetaboCard
PAMDB000942	4-Methyl-5-(2-phosphoethyl)-thiazole	MetaboCard
PAMDB000014	Adenosine monophosphate	MetaboCard
PAMDB000148	Adenosine triphosphate	MetaboCard
PAMDB000568	Carbon dioxide	MetaboCard
PAMDB001730	Dehydroglycine	MetaboCard
PAMDB000308	FAD	MetaboCard
PAMDB000293	FADH2	MetaboCard
PAMDB001633	Hydrogen ion	MetaboCard
PAMDB000172	Iron	MetaboCard
PAMDB000063	L-Alanine	MetaboCard
PAMDB000152	L-Cysteine	MetaboCard
PAMDB000197	NAD	MetaboCard
PAMDB000398	NADH	MetaboCard
PAMDB000089	NADP	MetaboCard
PAMDB000615	NADPH	MetaboCard
PAMDB000633	Pyrophosphate	MetaboCard
PAMDB000142	Water	MetaboCard

GO Classification:

Function
binding
catalytic activity
cofactor binding
cysteine desulfurase activity
pyridoxal phosphate binding
sulfurtransferase activity
transferase activity
transferase activity, transferring sulfur-containing groups
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
cysteine metabolic process
metabolic process
sulfur amino acid metabolic process

Gene Properties

Locus tag:

PA3814

Strand:

Entrez Gene ID:

879918

Accession:

NP_252503.1

GI:

15599009

Sequence start:

4270921

Sequence End:

4272135

Sequence Length:

1214

Gene Sequence:

>PA3814
ATGAAATTGCCGATCTACCTCGATTACTCCGCGACCACTCCCGTCGATCCCCGCGTCGCCCAGAAAATGAGCGAATGCCTGCTGATGGACGGCAACTTCGGCAACCCGGCTTCGCGTTCCCACGTGTTCGGCTGGAAAGCCGAAGAAGCCGTGGAGAACGCCCGTCGCCAGGTGGCCGAGCTGGTCAACGCCGATCCCCGCGAGATCGTCTGGACCTCCGGCGCCACCGAGTCCGACAACCTGGCGATCAAGGGCGTCGCGCACTTCTACTCGGGCAAGGGCAAGCACATCATCACCTCGAAGATCGAGCACAAGGCGGTCCTCGACACCTGCCGCCAGCTGGAGCGCGAAGGTTTCGAAGTGACCTACCTGGAGCCGGGAGAAGATGGCCTGATCACTCCGGCTCTGGTCGAGGCGGCGCTGCGTGACGACACCATCCTGGTCTCGGTCATGCACGTGAACAACGAGATCGGCACCGTCAACGACATCGCCGCCATCGGCGAGCTGACCCGCTCCCGCGGCGTGCTGTTCCACGTCGACGCCGCACAGTCGACCGGCAAGGTCGAGATCGACCTGGACAAGCTCAAGGTCGACCTGATGTCCTTCTCCGCGCACAAGACCTACGGCCCGAAAGGCATTGGCGCGCTCTACGTCCGGCGCAAGCCGCGCGTCCGCATCGAGGGTCAGATGCACGGTGGCGGCCATGAGCGCGGCATGCGTTCCGGCACCCTAGCCACCCACCAGATCGTCGGCATGGGCGAGGCGTTCCGCATCGCCAAGGAAGAAATGGCCCAGGAGAACGCCCGCGTGCTGGCGCTCCGCGACCGCTTCTTCGCGCAGATCGACGGCCTGGAAGAGCTCTACATCAACGGCAGCATGACCTCCCGCGTCCCGCACAACCTCAACGTCAGCTTCAACTACGTCGAGGGCGAGTCGCTGATCATGGCGCTCAAGGACCTCGCGGTCTCCTCCGGCTCGGCCTGTACCTCGGCGTCCCTGGAACCGTCCTACGTATTGCGCGCCCTGGGCCGCAACGACGAACTGGCGCATAGCTCCATCCGCTTCACCTTCGGTCGCTTCACCACCGAGGAAGAGATCGACTATGCCGCGAAGAAGGTGGTCGAGGCCGTTTCCAAGCTGCGCGAACTGTCGCCGCTGTGGGATATGTACAAAGAGGGCGTCGACCTGTCCCAGGTCGAATGGCAGGCGCACTGA

Protein Properties

Protein Residues:

404

Protein Molecular Weight:

44.7 kDa

Protein Theoretical pI:

5.92

Hydropathicity (GRAVY score):

-0.253

Charge at pH 7 (predicted):

-7.94

Protein Sequence:

>PA3814
MKLPIYLDYSATTPVDPRVAQKMSECLLMDGNFGNPASRSHVFGWKAEEAVENARRQVAELVNADPREIVWTSGATESDNLAIKGVAHFYSGKGKHIITSKIEHKAVLDTCRQLEREGFEVTYLEPGEDGLITPALVEAALRDDTILVSVMHVNNEIGTVNDIAAIGELTRSRGVLFHVDAAQSTGKVEIDLDKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEGQMHGGGHERGMRSGTLATHQIVGMGEAFRIAKEEMAQENARVLALRDRFFAQIDGLEELYINGSMTSRVPHNLNVSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRFTTEEEIDYAAKKVVEAVSKLRELSPLWDMYKEGVDLSQVEWQAH

References

External Links:

Resource	Link
Genome ID:	PA3814
Entrez Gene ID:	879918
NCBI Protein ID:	15599009

General Reference:

PaperBLAST - Find papers about PA3814 and its homologs

Cysteine desulfurase (PA3814)