Bifunctional protein putA (PA0782)

Identification
Name: Bifunctional protein putA
Synonyms:
  • Proline dehydrogenase
  • Proline oxidase
  • Delta-1-pyrroline-5-carboxylate dehydrogenase
  • P5C dehydrogenase
Gene Name: putA
Enzyme Class:
Biological Properties
General Function: Involved in oxidoreductase activity
Specific Function: Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon
Cellular Location: Not Available
KEGG Pathways:
  • Alanine, aspartate and glutamate metabolism pae00250
  • Arginine and proline metabolism pae00330
KEGG Reactions:
Thumb+2 Thumb+Thumb → Thumb+Thumb+Thumb
L-D-1-Pyrroline-5-carboxylic acid + 2 Water + NAD → L-Glutamate + Hydrogen ion + NADH
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
L-Glutamic-gamma-semialdehyde + NAD + Water ↔ L-Glutamate + NADH + Hydrogen ion
ReactionCard
Thumb+Thumb+2 Thumb ↔ Thumb+Thumb+Thumb
L-D-1-Pyrroline-5-carboxylic acid + NAD + 2 Water ↔ L-Glutamate + NADH + Hydrogen ion
ReactionCard
Thumb+Thumb+2 Thumb ↔ Thumb+Thumb+Thumb
L-D-1-Pyrroline-5-carboxylic acid + NADP + 2 Water ↔ L-Glutamate + NADPH + Hydrogen ion
ReactionCard
Thumb+Acceptor+Thumb ↔ Thumb+Reduced acceptor+Thumb+Thumb
L-Proline + Acceptor + Quinone ↔ L-D-1-Pyrroline-5-carboxylic acid + Reduced acceptor + (S)-1-pyrroline-5-carboxylate + Hydroquinone
ReactionCard
Thumb+Thumb+2 Thumb ↔ L-erythro-4-Hydroxyglutamate+Thumb+Thumb
Pyrroline hydroxycarboxylic acid + NAD + 2 Water ↔ L-erythro-4-Hydroxyglutamate + NADH + Hydrogen ion
ReactionCard
Thumb+Thumb+2 Thumb ↔ L-erythro-4-Hydroxyglutamate+Thumb+Thumb
Pyrroline hydroxycarboxylic acid + NADP + 2 Water ↔ L-erythro-4-Hydroxyglutamate + NADPH + Hydrogen ion
ReactionCard
SMPDB Reactions:
L-Proline+Thumb+Thumb → Thumb+Thumb+1-Pyrroline-5-carboxylic acid+Thumb
L-Proline + Ubiquinone-1 + L-Proline → Hydrogen ion + Ubiquinol-1 + 1-Pyrroline-5-carboxylic acid + L-D-1-Pyrroline-5-carboxylic acid
ReactionCard
Thumb+Thumb+Thumb → 2 Thumb+Thumb+L-Glutamic acid+Thumb
L-Glutamic-gamma-semialdehyde + NAD + Water → 2 Hydrogen ion + NADH + L-Glutamic acid + L-Glutamate
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+2 Thumb+Thumb → Thumb+Thumb+Thumb
L-D-1-Pyrroline-5-carboxylic acid + 2 Water + NAD → L-Glutamate + Hydrogen ion + NADH
ReactionCard
Complex Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
FAD + L-Proline → L-D-1-Pyrroline-5-carboxylic acid + FADH2 + Hydrogen ion
ReactionCard
Thumb+acceptor → Thumb+reduced acceptor
L-Proline + acceptor → (S)-1-pyrroline-5-carboxylate + reduced acceptor
ReactionCard
Thumb+NAD(P)(+)+2 Thumb → Thumb+NAD(P)H
(S)-1-pyrroline-5-carboxylate + NAD(P)(+) + 2 Water → L-Glutamate + NAD(P)H
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB003496(S)-1-pyrroline-5-carboxylateMetaboCard
PAMDB000308FADMetaboCard
PAMDB000293FADH2MetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000459HydroquinoneMetaboCard
PAMDB000329L-D-1-Pyrroline-5-carboxylic acidMetaboCard
PAMDB000055L-GlutamateMetaboCard
PAMDB000331L-Glutamic-gamma-semialdehydeMetaboCard
PAMDB000064L-ProlineMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
PAMDB000359Pyrroline hydroxycarboxylic acidMetaboCard
PAMDB003450QuinoneMetaboCard
PAMDB001968Ubiquinol-1MetaboCard
PAMDB001833Ubiquinone-1MetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
1-pyrroline-5-carboxylate dehydrogenase activity
catalytic activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-NH group of donors
oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor
proline dehydrogenase activity
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glutamate biosynthetic process
glutamate metabolic process
glutamine family amino acid metabolic process
metabolic process
oxidation reduction
proline biosynthetic process
proline catabolic process
proline metabolic process
Gene Properties
Locus tag: PA0782
Strand: +
Entrez Gene ID: 878503
Accession: NP_249473.1
GI: 15595979
Sequence start: 851783
Sequence End: 854965
Sequence Length: 3182
Gene Sequence: 
>PA0782
ATGTTCAAAGCCAGTCACGTCTTGCAGGGCGAGGACCAGACCCGGTCCGCAGCCGAATTCTTCCCTGTCATCAGCGCCAACTACGCGGTCGACGAGGAAGCCTACCTGGGCGAACTGCTGCAGCTCGCCGACCCCGGTCCCGACGGCATCGAGGCTATCCGCCGCAACGCCCGCGCACTGATCGAAACCGTGCGCAGCCGGGACAACGCCGTCGATACCCTCGACGCACTCCTGCGCCAATACAGCCTGGACACCCAGGAAGGCCTGATGCTGATGTGTCTGGCCGAGGCGCTGTTGCGTGTGCCTGACGCGGCGACCGCCGACGCGCTGATCCGCGACAAGCTGTCCGCCGCCGACTGGCAGCGCCATCTCGGCCAGAGCGACAACGTTCTGGTCAATTTCGCCGCCTGGGGCCTGGTGATGACCGGCAAGGTGGTGAACATCGACGAACGCACCGACGGCCGCGCCCCGAGCGTCATCAGCCGGCTGGTGCAGCGCTCTGGCGAACCGGTGATCCGCGCCGCGATGAACCAGGCGATGAAGCTGATGGGCAAGCAGTTCGTGCTTGGCCGGACCATCTCCGAAGCGCTGAAGAACGGTCGTCCATGCCGCGAGCAGGGCTACACCTATTCCTTCGACATGCTCGGCGAGGCCGCGCTGACCGCCGCCGACGCCGAGAAATACATGGCCGACTACCGCCAGGCCATCGACACCGTCGGCGCCGAGCCGCAGGTCGGTCCGGGGCCGCGGCCGTCGATCTCGATCAAGCTCTCGGCGCTGCATCCGCGCTACGAAGTGGCGCAACGCGAGCGGGTGCTCAGCGAGCTGTTCGCCAATGTCCTGGAACTGGCGGTGCGCGCGCGCAAGCTGAACGTCGGCATCACCATCGACGCCGAGGAAGCCGATCGCCTGGAGCTGTCCCTCGAACTCTACGAGAAGCTGATGCGCGACCCGGCCATCGCCGGCTGGGGCGAGTTCGGCCTGGTCATCCAGGCCTACTCCAAGCGCTGCCTGCCGGTGCTGGTATGGCTGACCCTGCTCGGCCGCGAGCTTGGCGCACGCATCCCGCTGCGCCTGGTGAAGGGTGCCTACTGGGACAGCGAGATCAAGCAGTGCCAGGTGCAGGGCGCCGATGGCTACCCCGTCTACACCCGCAAGGAAGGCACCGACACTTCCTACCTGGCCTGCGCCCGCTACCTGCTCAGCGAGCACACCCGCGGGGTGATCTACCCGCAGTTCGCCAGCCACAACGCCCACACCGTGACCGCCATCCTCGCCCTGGCCGACGAAGCCAGGGCCGCCGGCGGCGAGGAGCGCGACTTCGAGTTCCAGCGCCTGCACGGCATGGGCGACGCGCTGTACGACACGGTGATCGAAAAGTACCGCCGCAACGTGCGCATCTACGCCCCGGTCGGCGCGCACAAGGACCTGCTGCCGTACCTGGTCCGCCGCCTGCTGGAGAACGGCGCCAACTCTTCCTTCGTGCACAAGCTGGTGGACCCGCGCGTGCCGGTGGAGACGCTGATCCAGCACCCGGTTACCCAACTGCGCCAGTTCAAGACGCTGGCCAACGACCGTATCCCTCTGCCCCAGGCGATCTTCGGCGCCGGGCGGAAAAACTCCCAGGGCATCAACATGAACATCCAGAACCAGTGGAACGAGCTGGAGCTCGCCTACAAGCCGCACCTGACCCGCCAGTGGCAAGCCGCGCCGATCGTCAACGGCGAGAAGCTCTCCGGCCCGGCCCACGAAGTGCGTTGCCCGTACGACCTGGAGAGGCTGGTCGGCAGCGTCCAGTACGCGTCCGCCGAGCAGGCCGGCAAGGCCCTCGACGTGTTGCAGGCGGCCTGGCCGCGCTGGAGCGCCACCCCGGTGGACGAGCGTGCCGGCATCCTCGAGCGTCTCGCCGACCTGCTGGAAGCCCAGCGCGGCGAGCTGATGGCGCTGTGCACCCTGGAAGCCGGCAAGTCGTTGCAGGATGGTATCGACGAGATCCGCGAGGCGGTCGACTTCTGCCGCTACTACGCCCAGCAGGCCCGCCAGCGCCTGGTCCGCGAGGAACTGCGCGGCCCGACCGGCGAGCGCAACGAGCTGTTCTACGAAGGCCGCGGCATCTTCGCCTGCGTCAGCCCGTGGAACTTCCCGCTGGCGATCTTCCTCGGCCAGATCAGCGCCGCGCTGGTGGCCGGCAACACCGTGCTGGCCAAGCCGGCCGAGCAGACCAGCCTGATCGCCGCGCGCACCGTCGAACTGATGTTCGAAGCCGGCCTGCCGAAGGACGTTATCGCCCTGCTGCCGGGCGACGGCGCCACCCTCGGCGGCGTGTTCTGCCGTGACGCGCGGGTCGCCGGGGTGGCCTTCACCGGCTCCACCGACACCGCCCGCATCATCAACCGCCAACTGGCCGAGAAGCCCGGCGCCATCGCCACCCTGATCGCCGAGACCGGCGGGCAGAACGCGATGATCGTCGATTCCACCGCTTTGCCGGAACAGGTGATCAAGGATGCGGTACAATCCGCGTTCACCAGCGCCGGCCAGCGCTGCTCCGCGCTGCGCGTGATGTACGTGCAGCAGGACATCGCCGAGCGGGTGATCGAGTTGCTGAAGGGCGCCATGGCCGAGCTGAAGGTCGGCCCGACCGAGGTGCGCGTCAGCGACGTCGGCCCGGTGATCGACGCCGAGGCCAAGGCCGGCCTGGAGCAGCACATCGCCGCGCTGAAGGCCGCCGGCAAGCTGATCGCCGAGACCAAGGTGCCGGGCAACCTGAACGGTCACTTCGTCGCTCCCGTCGCGTTCGAGATCAGCGGCATCGACGAGCTGAAGAAAGAGAACTTCGGCCCGGTGCTGCACGTGGTGCGTTATGCCGCGCAGGACCTGGAAAAGGTCGTGCAGGCGATCAACGCCACCGGTTTCGGCCTGACCATGGGGGTCCATAGCCGCAACGAGGAAACCGCCCGTCGCATCGAAGAGCTGGCGCGGGTCGGCAACCTCTACATCAACCGCAACCAGATCGGCGCGGTGGTGGGCGTGCAACCGTTCGGCGGCCACGGCCTGTCCGGCACCGGTCCCAAGGCCGGCGGGCCGAACTACCTGCTGCGTTTCGTCAGCGAGCGCACCACGTCGGTGAACACCACCGCGGTGGGCGGCAACGCGTCGCTGCTGTCGCTGGCCGACGCCGAGTGA
Protein Properties
Protein Residues: 1060
Protein Molecular Weight: 115.6 kDa
Protein Theoretical pI: 6.02
Hydropathicity (GRAVY score): -0.119
Charge at pH 7 (predicted): -10.17
Protein Sequence: 
>PA0782
MFKASHVLQGEDQTRSAAEFFPVISANYAVDEEAYLGELLQLADPGPDGIEAIRRNARALIETVRSRDNAVDTLDALLRQYSLDTQEGLMLMCLAEALLRVPDAATADALIRDKLSAADWQRHLGQSDNVLVNFAAWGLVMTGKVVNIDERTDGRAPSVISRLVQRSGEPVIRAAMNQAMKLMGKQFVLGRTISEALKNGRPCREQGYTYSFDMLGEAALTAADAEKYMADYRQAIDTVGAEPQVGPGPRPSISIKLSALHPRYEVAQRERVLSELFANVLELAVRARKLNVGITIDAEEADRLELSLELYEKLMRDPAIAGWGEFGLVIQAYSKRCLPVLVWLTLLGRELGARIPLRLVKGAYWDSEIKQCQVQGADGYPVYTRKEGTDTSYLACARYLLSEHTRGVIYPQFASHNAHTVTAILALADEARAAGGEERDFEFQRLHGMGDALYDTVIEKYRRNVRIYAPVGAHKDLLPYLVRRLLENGANSSFVHKLVDPRVPVETLIQHPVTQLRQFKTLANDRIPLPQAIFGAGRKNSQGINMNIQNQWNELELAYKPHLTRQWQAAPIVNGEKLSGPAHEVRCPYDLERLVGSVQYASAEQAGKALDVLQAAWPRWSATPVDERAGILERLADLLEAQRGELMALCTLEAGKSLQDGIDEIREAVDFCRYYAQQARQRLVREELRGPTGERNELFYEGRGIFACVSPWNFPLAIFLGQISAALVAGNTVLAKPAEQTSLIAARTVELMFEAGLPKDVIALLPGDGATLGGVFCRDARVAGVAFTGSTDTARIINRQLAEKPGAIATLIAETGGQNAMIVDSTALPEQVIKDAVQSAFTSAGQRCSALRVMYVQQDIAERVIELLKGAMAELKVGPTEVRVSDVGPVIDAEAKAGLEQHIAALKAAGKLIAETKVPGNLNGHFVAPVAFEISGIDELKKENFGPVLHVVRYAAQDLEKVVQAINATGFGLTMGVHSRNEETARRIEELARVGNLYINRNQIGAVVGVQPFGGHGLSGTGPKAGGPNYLLRFVSERTTSVNTTAVGGNASLLSLADAE
References
External Links:
Resource Link
Genome ID: PA0782
Entrez Gene ID: 878503
NCBI Protein ID: 15595979
General Reference: PaperBLAST - Find papers about PA0782 and its homologs