Pyridoxine 5'-phosphate synthase (PA0773)

Identification
Name: Pyridoxine 5'-phosphate synthase
Synonyms:
  • PNP synthase
Gene Name: pdxJ
Enzyme Class:
Biological Properties
General Function: Involved in catalytic activity
Specific Function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
3-Amino-2-oxopropyl phosphate+Thumb+Thumb ↔ Thumb+Thumb+2 Thumb
3-Amino-2-oxopropyl phosphate + 1-Deoxy-D-xylulose 5-phosphate + 3-Amino-2-oxopropyl phosphate ↔ Pyridoxine 5'-phosphate + Phosphate + 2 Water
ReactionCard
SMPDB Reactions:
1-Deoxy-D-xylulose 5-phosphate+2-Amino-3-phosphonopropionic acid+Thumb+2-Amino-3-phosphonopropionic acid → Thumb+Thumb+Thumb+2 Thumb
1-Deoxy-D-xylulose 5-phosphate + 2-Amino-3-phosphonopropionic acid + 1-Deoxy-D-xylulose 5-phosphate + 2-Amino-3-phosphonopropionic acid → Pyridoxine 5'-phosphate + Phosphate + Hydrogen ion + 2 Water
ReactionCard
PseudoCyc/BioCyc Reactions:
3-Amino-2-oxopropyl phosphate+Thumb+Thumb ↔ Thumb+Thumb+2 Thumb
3-Amino-2-oxopropyl phosphate + 1-Deoxy-D-xylulose 5-phosphate + 3-Amino-2-oxopropyl phosphate ↔ Pyridoxine 5'-phosphate + Phosphate + 2 Water
ReactionCard
1-Deoxy-D-xylulose 5-phosphate+2-Amino-3-phosphonopropionic acid+Thumb+2-Amino-3-phosphonopropionic acid → Thumb+Thumb+Thumb+2 Thumb
1-Deoxy-D-xylulose 5-phosphate + 2-Amino-3-phosphonopropionic acid + 1-Deoxy-D-xylulose 5-phosphate + 2-Amino-3-phosphonopropionic acid → Pyridoxine 5'-phosphate + Phosphate + Hydrogen ion + 2 Water
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Thumb
1-deoxy-D-xylulose 5-phosphate = pyridoxine 5'-phosphate + CO2
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Complex Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+2 Thumb+Thumb+Thumb+Thumb
1-Deoxy-D-xylulose 5-phosphate + NAD + O-Phospho-4-hydroxy-L-threonine → Carbon dioxide + Hydrogen ion + 2 Water + NADH + Pyridoxine 5'-phosphate + Phosphate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0003011-Deoxy-D-xylulose 5-phosphateMetaboCard
PAMDB0042503-Amino-2-oxopropyl phosphateMetaboCard
PAMDB000568Carbon dioxideMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001776Inorganic phosphateMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000691O-Phospho-4-hydroxy-L-threonineMetaboCard
PAMDB000383PhosphateMetaboCard
PAMDB000338Pyridoxine 5'-phosphateMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
Process
metabolic process
pyridoxine biosynthetic process
pyridoxine metabolic process
small molecule metabolic process
vitamin B6 metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process
Gene Properties
Locus tag: PA0773
Strand: +
Entrez Gene ID: 878131
Accession: NP_249464.1
GI: 15595970
Sequence start: 841079
Sequence End: 841825
Sequence Length: 746
Gene Sequence: 
>PA0773
GTGACTGAAGCCACTCGAATTCTGCTCGGCGTGAACATCGATCACGTCGCCACCCTGCGCCAGGCCCGGGGCACCCGCTATCCGGACCCGGTGAAGGCCGCCCTGGACGCCGAGGAAGCCGGCGCCGACGGTATCACCGTGCACCTGCGCGAGGACCGCCGGCATATCCAGGAACGCGACGTGCGGGTGCTCAAGGAAGTGCTGCAGACGCGGATGAACTTCGAGATGGGGGTCACCGAGGAAATGCTCGCCTTCGCCGAGGAGATCCGGCCGGCGCATAGCTGCCTGGTCCCCGAGCGCCGCGAGGAACTGACCACCGAGGGCGGCCTCGACGTCGCCGGCCAGGAACAGCGCATCCGCGACGCGGTGCGACGCCTGGCTGCGGTGGGCAGCGAAGTGTCGCTGTTCATCGATCCCGATCCGCGGCAGATCGAGGCCTCCGCCCGGGTCGGCGCGCCGGCTATCGAACTGCATACCGGTCGCTACGCCGACGCCGAAGACCCCGAGGAGCAGGCCCGCGAGCTGCAGCGGGTACGCGAGGGCGTGGCCCTCGGGCGTTCCCTCGGCCTGATCGTCAACGCCGGGCATGGCCTGCACTACCACAATGTCGAGCCGGTCGCGGCCATCGACGGCATCAACGAGCTGAACATCGGCCACGCCATCGTCGCCCACGCCCTCTTCGTCGGCTTCAGGCAGGCGGTGGCCGAGATGAAAGCGCTGATGCTGGCCGCGGCGACCAAGCGCTGA
Protein Properties
Protein Residues: 248
Protein Molecular Weight: 27.2 kDa
Protein Theoretical pI: 5.59
Hydropathicity (GRAVY score): -0.237
Charge at pH 7 (predicted): -7.61
Protein Sequence: 
>PA0773
MTEATRILLGVNIDHVATLRQARGTRYPDPVKAALDAEEAGADGITVHLREDRRHIQERDVRVLKEVLQTRMNFEMGVTEEMLAFAEEIRPAHSCLVPERREELTTEGGLDVAGQEQRIRDAVRRLAAVGSEVSLFIDPDPRQIEASARVGAPAIELHTGRYADAEDPEEQARELQRVREGVALGRSLGLIVNAGHGLHYHNVEPVAAIDGINELNIGHAIVAHALFVGFRQAVAEMKALMLAAATKR
References
External Links:
Resource Link
Genome ID: PA0773
Entrez Gene ID: 878131
NCBI Protein ID: 15595970
General Reference: PaperBLAST - Find papers about PA0773 and its homologs