Bifunctional (p)ppGpp synthase/hydrolase SpoT (PA5338)

Identification
Name: Bifunctional (p)ppGpp synthase/hydrolase SpoT
Synonyms:
  • GTP pyrophosphokinase
  • (p)ppGpp synthase
  • ATP:GTP 3'-pyrophosphotransferase
  • Stringent response-like protein
  • ppGpp synthase II
  • Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
  • Penta-phosphate guanosine-3'-pyrophosphohydrolase
  • (ppGpp)ase
Gene Name: spoT
Enzyme Class:
Biological Properties
General Function: Involved in catalytic activity
Specific Function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the synthesis and degradation of ppGpp. The second messengers ppGpp and c-di-GMP together control biofilm formation in response to translational stress; ppGpp represses biofilm formation while c- di-GMP induces it
Cellular Location: Cytoplasm.
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb+Thumb
Adenosine triphosphate + Guanosine triphosphate ↔ Adenosine monophosphate + Guanosine 3'-diphosphate 5'-triphosphate + Hydrogen ion
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
Water + Guanosine 3',5'-bis(diphosphate) ↔ Guanosine diphosphate + Pyrophosphate
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
Guanosine 3',5'-bis(diphosphate) + Water ↔ Guanosine diphosphate + Pyrophosphate
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SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
Adenosine triphosphate + Guanosine diphosphate → Adenosine monophosphate + Hydrogen ion + Guanosine 3',5'-bis(diphosphate)
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
Adenosine triphosphate + Guanosine triphosphate ↔ Adenosine monophosphate + Guanosine 3'-diphosphate 5'-triphosphate + Hydrogen ion
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
Water + Guanosine 3',5'-bis(diphosphate) ↔ Guanosine diphosphate + Pyrophosphate
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Thumb+Thumb → Thumb+Thumb
Guanosine 3'-diphosphate 5'-triphosphate + Water → Guanosine triphosphate + Pyrophosphate
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Thumb+Thumb → Thumb+Thumb
Adenosine triphosphate + Guanosine diphosphate → Adenosine monophosphate + Guanosine 3',5'-bis(diphosphate)
ReactionCard
Thumb+Thumb → Thumb+Thumb+Thumb
Guanosine 3',5'-bis(diphosphate) + Water → Hydrogen ion + Pyrophosphate + Guanosine diphosphate
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Thumb+Thumb → Thumb+Thumb+Thumb
Guanosine 3'-diphosphate 5'-triphosphate + Water → Hydrogen ion + Guanosine triphosphate + Pyrophosphate
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Complex Reactions:
Thumb+Thumb → Thumb+Thumb
Adenosine triphosphate + Guanosine triphosphate → Adenosine monophosphate + Guanosine 3'-diphosphate 5'-triphosphate
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Thumb+Thumb → Thumb+Thumb
Guanosine 3',5'-bis(diphosphate) + Water → Guanosine diphosphate + Pyrophosphate
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB001005Guanosine 3',5'-bis(diphosphate)MetaboCard
PAMDB001003Guanosine 3'-diphosphate 5'-triphosphateMetaboCard
PAMDB000296Guanosine diphosphateMetaboCard
PAMDB000319Guanosine triphosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000633PyrophosphateMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
catalytic activity
Process
cellular nitrogen compound metabolic process
guanosine metabolic process
guanosine tetraphosphate metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside metabolic process
purine ribonucleoside metabolic process
ribonucleoside metabolic process
Gene Properties
Locus tag: PA5338
Strand: +
Entrez Gene ID: 877912
Accession: NP_254025.1
GI: 15600531
Sequence start: 6006231
Sequence End: 6008336
Sequence Length: 2105
Gene Sequence: 
>PA5338
TTGCCGGGCATAGACGCCTTCGCCGACAGACTGTCGAGCTACCTCGGGCCGGACCAGGTCAACCTGGTCCGCCGCGCCTACTACTACGCCGAGCAGGCCCACGACGGGCAACGCCGCCGCAGCGGCGAACCGTACGTGACCCACCCGCTGGCGGTCTCCAACATCCTCGCCGACATGCACATGGACCATCAGAGCCTGATGGCCGCGATGCTGCACGACGTGATCGAGGACACCGGCATCGCCAAGGAAGCGCTCACCGCGCAGTTCGGCGAGACGGTTTCGGAACTGGTCGACGGGGTCAGCAAGCTGACCCAGATGAACTTCGAGACCAAGGCCGAGGCCCAGGCCGAGAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGCCGACCGCCTGCACAACATGCGCACCCTCGAGGTGCTGTCCGGCGAAAAGCGCCGACGCATCGCCAAGGAAACCCTGGAAATCTACGCGCCCATCGCCAACCGCCTGGGCATGCACACCATGCGCGTGGAGTTCGAGGACCTAGGTTTCAAGGCCATGCACCCGATGCGCGCCGAGCGCATCCGCCAGGCGGTGAAGAAGGCCCGCGGCAACCGCCGCGAGATCGTCGGCAAGATCCAGGAGTCGCTGGTCAACTGCCTCGCCCGGGAAGGCATGGAAGGCCAGGTGATCGGCCGCGAGAAGCACCTCTACAGCATCTACCAGAAGATGCGCGGCAAGCGTAAGGCGTTCAACGAGATCATGGACGTCTACGCCTTCCGCATCATCGTCGACAAGGTCGATACCTGCTATCGCGTGCTGGGCGCCGTGCACAGCCTGTACAAGCCGTTCCCCGGACGCTTCAAGGACTACATCGCGATTCCCAAGGCCAACGGCTACCAGTCGCTACATACCACCCTGTTCGGCATGCACGGGGTGCCCATCGAGATCCAGATCCGTACCCGCGAGATGGAAGAGATGGCCAACCACGGGATCGCCGCGCACTGGCTGTACAAGTCCAACGACGAGACGCCCAAGGGCACCCATGCCCGCGCCCGGCAATGGGTGAAGGGCGTGCTGGAACTGCAGCAGCGCGCCGGCAACTCGCTGGAATTCATCGAGAACGTGAAGATCGACCTGTTCCCGGACGAGGTCTACGTGTTCACGCCCAAGGGCCGCATCATGGAACTGCCCAAGGGTTCCACCGCGGTCGACTTCGCCTACGCGGTGCACACTGACGTCGGCAACAGTTGCATCGCCTGCCGCATCAACCGTCGCCTGGCGCCGCTCTCCGAGCCGTTGCAGAGCGGCCAGACGGTGGAAATCGTCACCGCCCCGGGCGCCCGGCCGAACCCGGCCTGGCTGAGCTTCGTGGTCACCGGCAAGGCGCGCACCCATATCCGCCATGCGCTCAAGCTGCAACGCCGCTCCGAGTCGATCAACCTCGGCGAGCGCCTGCTGAACAAGACCCTTACCGGCTTCGACAGCCACCTCGAGAAGATTCCCCAGGAACGCATCCAGGCCGTGCTCGCCGAGTACCGCATGGAAGTCTTCGAGGACCTGCTGGAAGAGATCGGCCTGGGCAACCGCATGGCCTACGTGGTCGCCCGCCGCCTGCTGTCCAGCGAGGGCGAGGAAGCGCCAAGCGCCGAGGGTCCGCTGGCGATCCGCGGCACCGAAGGCCTGGTCCTGAGCTACGCCAAGTGCTGCACGCCGATTCCCGGCGACCCGATCGTTGGCCACCTGTCCGCCGGCAAGGGCATGGTGGTGCACCTGGAAAGCTGCAAGAACATCAGCGAAGTACGCCACAACCCGGAAAAATGCATCCAGCTCAGCTGGGCCAAGGATGTCACCGGCGAATTCAATGTCGAACTGCGCGTCGAGCTGGAGCACCAGCGTGGCCTGATCGCCCTGCTCGCCACCAGCGTCAACGCGGCCGACGGCAACATCGAGAAGATCAGCATGGACGAGCGCGACGGCCGCATCAGCGTGGTCCAGCTGGTCGTCAGCGTGCACGATCGCGTCCACCTGGCCCGCGTCATCAAGAAGCTGCGCGCCCTCAAGGGAGTGATCCGAATCACCCGCCTGCGTAGCTGA
Protein Properties
Protein Residues: 701
Protein Molecular Weight: 78.9 kDa
Protein Theoretical pI: 9.35
Hydropathicity (GRAVY score): -0.275
Charge at pH 7 (predicted): 17.81
Protein Sequence: 
>PA5338
MPGIDAFADRLSSYLGPDQVNLVRRAYYYAEQAHDGQRRRSGEPYVTHPLAVSNILADMHMDHQSLMAAMLHDVIEDTGIAKEALTAQFGETVSELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAKETLEIYAPIANRLGMHTMRVEFEDLGFKAMHPMRAERIRQAVKKARGNRREIVGKIQESLVNCLAREGMEGQVIGREKHLYSIYQKMRGKRKAFNEIMDVYAFRIIVDKVDTCYRVLGAVHSLYKPFPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEIQIRTREMEEMANHGIAAHWLYKSNDETPKGTHARARQWVKGVLELQQRAGNSLEFIENVKIDLFPDEVYVFTPKGRIMELPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGQTVEIVTAPGARPNPAWLSFVVTGKARTHIRHALKLQRRSESINLGERLLNKTLTGFDSHLEKIPQERIQAVLAEYRMEVFEDLLEEIGLGNRMAYVVARRLLSSEGEEAPSAEGPLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLESCKNISEVRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIALLATSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALKGVIRITRLRS
References
External Links:
Resource Link
Genome ID: PA5338
Entrez Gene ID: 877912
NCBI Protein ID: 15600531
General Reference: PaperBLAST - Find papers about PA5338 and its homologs