Identification
Name: 3-deoxy-D-manno-octulosonic-acid transferase
Synonyms:
  • KDO transferase
Gene Name: waaA
Enzyme Class:
Biological Properties
General Function: Involved in biosynthetic process
Specific Function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA. Degraded by FtsH; therefore FtsH regulates the addition of the sugar moiety of the LPS and thus the maturation of the LPS precursor
Cellular Location: Cell inner membrane; Single-pass membrane protein (Probable)
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+ThumbThumb+Thumb
Thumb+ThumbDi[3-deoxy-D-manno-octulosonyl]-lipid IV(A)+Thumb
KDO-lipid IV(A) + CMP-3-Deoxy-D-manno-octulosonate ↔ Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A) + Cytidine monophosphate
ReactionCard
Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A)+Thumbalpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA+Thumb
Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A) + CMP-3-Deoxy-D-manno-octulosonate ↔ alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + Cytidine monophosphate
ReactionCard
SMPDB Reactions:
Thumb+ThumbCytidine monophosphate+Thumb+Thumb+Thumb
Thumb+ThumbCytidine monophosphate+Thumb+a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA+Thumb+Thumb
PseudoCyc/BioCyc Reactions:
Thumb+lipid IVAThumb+Thumb+Thumb
Thumb+ThumbThumb+α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IVA+Thumb
Complex Reactions:
Thumb+ThumbThumb+Thumb+Thumb
Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumbalpha-Kdo-(2->6)-lipid IV(A)+Thumb
Alpha-Kdo-(2->6)-lipid IV(A)+Thumbalpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A)+Thumb
Alpha-Kdo-(2->6)-lipid IV(A) + CMP-3-Deoxy-D-manno-octulosonate → alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + Cytidine monophosphate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB003481(2-N,3-O-bis(3-Hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate)MetaboCard
PAMDB0009062,3,2'3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphateMetaboCard
PAMDB0015452,3,2'3'-Tetrakis(beta-hydroxymyristoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphateMetaboCard
PAMDB006295a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVAMetaboCard
PAMDB006335alpha-Kdo-(2??)-lipid IVAMetaboCard
PAMDB000981CMP-3-Deoxy-D-manno-octulosonateMetaboCard
PAMDB000035Cytidine monophosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001643KDO(2)-lipid IV(A)MetaboCard
PAMDB001644KDO-lipid IV(A)MetaboCard
GO Classification:
Function
binding
carbohydrate binding
catalytic activity
sugar binding
transferase activity
Process
biosynthetic process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Locus tag: PA4988
Strand: -
Entrez Gene ID: 880145
Accession: NP_253675.1
GI: 15600181
Sequence start: 5603821
Sequence End: 5605098
Sequence Length: 1277
Gene Sequence:
>PA4988
ATGAACCGTACCCTCTATACCCTGCTGTTCCACCTCGGCTTGCCGCTGGTGGCGTTGCGCCTGTGGTGGCGCGCGCGCCAGGCGCCGGCCTATGCCAAGCGCATCGGCGAGCGTTTTTCCCTGTCCTTGCCCGAGGTGCCGCCCGGCGGCATCTGGGTGCACGCCGTCTCGGTGGGCGAGAGCATCGCCGCCGCGCCGATGGTGCGGGCGCTGCTGGAGCGCCATCCGCAGCTGCCGGTCACCGTCACCTGCATGACCCCGACCGGCTCGGAGCGCATCCGCGCATTGTTCGGCGAGCAGGTCCGGCACTGCTACCTGCCCTACGACCTGCCCTGGGCGGCGGCGCGCTTCCTCGACCGGGTGCGGCCGCGCCTGGCGGTGATCATGGAAACCGAGCTGTGGCCCAACCATATCCATGCCTGCGCCGTGCGCGGGATCCCGGTGGCGCTGGCCAATGCGCGCCTGTCCGAGCGCTCGGCGCGTGGCTACGCGCGCTTCGCCGGGCTGACCCGGCCGATGCTCGCCGAGCTGTCCTGGATCGCCGTGCAGACCGAGGCCGAGGCCGAGCGCTTCCGTCGTCTCGGCGCGCGTCCTGAGTGCGTCAGCGTGACCGGCTCGATCAAGTTCGACCTGCGCATCGACCCGCAGCTGCCGCTGGCCGCTGCCGCCCTGCGCGAGGAATGGGACGCGACGGCGCGACCGCTGTGGATCGCCGCCAGCACCCATGCCGGCGAGGACGAGATCGTCCTGGCAGCCCACCGGCGCCTGCTGGAAACGCGTCCCGACGCGTTGCTGATCCTGGTGCCGCGCCATCCGGAACGCTTCGCCGGCGTCCACGAGCTATGCCGCCGCGAAGGCTTCGCCACGGTCCGGCGCTCCGGCGGCGAGCCGGTGGCACGCGCGACCCAGGTGCTGCTCGGCGATACCATGGGCGAGCTGCTGTTCCTCTATGCCCTGGCCGACATCGCTTTCGTCGGCGGCAGCCTGGTGCCCAACGGCGGGCACAACCTGCTGGAGCCGGCGGCCCTGGGCAAGCCGGTGTTCGCCGGTCCGCACCTGTTCAACTTCCTCGACATCGCCGCGCAATTGCGCGACGCCGGGGCGCTGCTCGAGGTGACGGATGCCGGCGAGCTGTGCGACGGCCTCGCGCGCCTGTGGGCGCAGCCGGAGGTCGCGACGGCCATGGCCACTGCCGGAGAGAAGGTGCTGCGCAACAACCAGGGCGCCCTGGAGCGGCTGCTGGCGGGGTTGGCGCGGTTGCTCGGGCGCGGCGGGTAG
Protein Properties
Protein Residues: 425
Protein Molecular Weight: 46.5 kDa
Protein Theoretical pI: 8.83
Hydropathicity (GRAVY score): 0.102
Charge at pH 7 (predicted): 7.72
Protein Sequence:
>PA4988
MNRTLYTLLFHLGLPLVALRLWWRARQAPAYAKRIGERFSLSLPEVPPGGIWVHAVSVGESIAAAPMVRALLERHPQLPVTVTCMTPTGSERIRALFGEQVRHCYLPYDLPWAAARFLDRVRPRLAVIMETELWPNHIHACAVRGIPVALANARLSERSARGYARFAGLTRPMLAELSWIAVQTEAEAERFRRLGARPECVSVTGSIKFDLRIDPQLPLAAAALREEWDATARPLWIAASTHAGEDEIVLAAHRRLLETRPDALLILVPRHPERFAGVHELCRREGFATVRRSGGEPVARATQVLLGDTMGELLFLYALADIAFVGGSLVPNGGHNLLEPAALGKPVFAGPHLFNFLDIAAQLRDAGALLEVTDAGELCDGLARLWAQPEVATAMATAGEKVLRNNQGALERLLAGLARLLGRGG
References
External Links:
Resource Link
Genome ID: PA4988
Entrez Gene ID: 880145
NCBI Protein ID: 15600181
General Reference: PaperBLAST - Find papers about PA4988 and its homologs