NH(3)-dependent NAD(+) synthetase (PA4920)

Identification
Name: NH(3)-dependent NAD(+) synthetase
Synonyms:
  • Nicotinamide adenine dinucleotide synthetase
  • NADS
  • Nitrogen regulatory protein
Gene Name: nadE
Enzyme Class:
Biological Properties
General Function: Involved in NAD+ synthase (glutamine-hydrolyzing) activity
Specific Function: Catalyzes a key step in NAD biosynthesis, transforming deamido-NAD into NAD by a two-step reaction
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Adenosine triphosphate + Nicotinic acid adenine dinucleotide + Ammonia ↔ Adenosine monophosphate + Pyrophosphate + NAD
ReactionCard
SMPDB Reactions:
Thumb+Thumb+Thumb+Thumb → Thumb+Thumb+Pyrophosphate+L-Glutamic acid+Thumb+Thumb
Nicotinic acid adenine dinucleotide + Water + L-Glutamine + Adenosine triphosphate → Hydrogen ion + Adenosine monophosphate + Pyrophosphate + L-Glutamic acid + NAD + L-Glutamate
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb+Pyrophosphate+Thumb
Nicotinic acid adenine dinucleotide + Adenosine triphosphate + Ammonium → Hydrogen ion + Adenosine monophosphate + Pyrophosphate + NAD
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
Adenosine triphosphate + Nicotinic acid adenine dinucleotide + Ammonia ↔ Adenosine monophosphate + Pyrophosphate + NAD
ReactionCard
Complex Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Adenosine triphosphate + Nicotinic acid adenine dinucleotide + Ammonium → Adenosine monophosphate + Hydrogen ion + NAD + Pyrophosphate
ReactionCard
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
Adenosine triphosphate + Nicotinic acid adenine dinucleotide + Ammonia → Adenosine monophosphate + Pyrophosphate + NAD
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000017AmmoniaMetaboCard
PAMDB001595AmmoniumMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000055L-GlutamateMetaboCard
PAMDB000163L-GlutamineMetaboCard
PAMDB000197NADMetaboCard
PAMDB000286Nicotinic acid adenine dinucleotideMetaboCard
PAMDB000633PyrophosphateMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
carbon-nitrogen ligase activity, with glutamine as amido-N-donor
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
NAD+ synthase (glutamine-hydrolyzing) activity
nucleoside binding
purine nucleoside binding
Process
cellular metabolic process
coenzyme biosynthetic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
NAD biosynthetic process
nicotinamide nucleotide biosynthetic process
pyridine nucleotide biosynthetic process
Gene Properties
Locus tag: PA4920
Strand: +
Entrez Gene ID: 882213
Accession: NP_253607.1
GI: 15600113
Sequence start: 5519711
Sequence End: 5520538
Sequence Length: 827
Gene Sequence: 
>PA4920
ATGCAACAGATCCAACGCGACATCGCCCAAGCCCTGCAGGTCCAGCCGCCGTTCCAGTCGGAGGCCGACGTGCAGGCGCAGATCGCCCGGCGCATCGCCTTCATCCAGCAGTGCCTGAAGGATTCCGGGCTGAAGACCCTGGTGCTGGGGATCAGCGGCGGCGTCGACTCGCTCACCGCCGGCCTGCTGGCCCAGCGCGCCGTCGAGCAACTGCGCGAGCAGACCGGCGACCAGGCCTACCGCTTCATCGCCGTGCGCCTGCCCTACCAGGTGCAGCAGGACGAGGCCGATGCCCAGGCCTCGCTGGCGACCATCCGCGCCGACGAAGAGCAGACCGTCAACATCGGTCCGTCGGTGAAAGCCCTGGCGGAACAGCTGGAAGCCCTGGAAGGACTCGAGCCGGCGAAGAGCGACTTCGTCATCGGCAATATCAAGGCGCGCATCCGCATGGTCGCCCAGTACGCCATCGCCGGCGCCCGCGGCGGCCTGGTGATCGGCACCGACCATGCTGCCGAGGCGGTCATGGGGTTCTTCACCAAGTTCGGCGACGGCGCCTGCGACCTGGCTCCGCTCAGCGGCCTGGCCAAGCATCAGGTACGCGCCCTCGCCCGCGCCCTCGGCGCTCCGGAGAACCTGGTGGAGAAGATCCCCACCGCCGACCTCGAGGACCTGCGCCCCGGCCATCCGGACGAGGCTTCCCACGGCGTCACCTATGCCGAGATCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCTGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGA
Protein Properties
Protein Residues: 275
Protein Molecular Weight: 29.7 kDa
Protein Theoretical pI: 5.38
Hydropathicity (GRAVY score): -0.193
Charge at pH 7 (predicted): -6.37
Protein Sequence: 
>PA4920
MQQIQRDIAQALQVQPPFQSEADVQAQIARRIAFIQQCLKDSGLKTLVLGISGGVDSLTAGLLAQRAVEQLREQTGDQAYRFIAVRLPYQVQQDEADAQASLATIRADEEQTVNIGPSVKALAEQLEALEGLEPAKSDFVIGNIKARIRMVAQYAIAGARGGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLAKHQVRALARALGAPENLVEKIPTADLEDLRPGHPDEASHGVTYAEIDAFLHGQPLREEAARVIVDTYHKTQHKRELPKAP
References
External Links:
Resource Link
Genome ID: PA4920
Entrez Gene ID: 882213
NCBI Protein ID: 15600113
General Reference: PaperBLAST - Find papers about PA4920 and its homologs