DNA-directed RNA polymerase subunit alpha (PA4238)

Identification
Name: DNA-directed RNA polymerase subunit alpha
Synonyms:
  • RNAP subunit alpha
  • RNA polymerase subunit alpha
  • Transcriptase subunit alpha
Gene Name: rpoA
Enzyme Class:
Biological Properties
General Function: Involved in DNA-directed RNA polymerase activity
Specific Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme
Cellular Location: Cytoplasmic
KEGG Pathways:
KEGG Reactions:
Thumb+RNA+RNA ↔ Thumb+RNA
Adenosine triphosphate + RNA + RNA ↔ Pyrophosphate + RNA
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Thumb+RNA ↔ Thumb+RNA
Guanosine triphosphate + RNA ↔ Pyrophosphate + RNA
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Thumb+RNA ↔ Thumb+RNA
Cytidine triphosphate + RNA ↔ Pyrophosphate + RNA
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Thumb+RNA ↔ Thumb+RNA
Uridine triphosphate + RNA ↔ Pyrophosphate + RNA
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Nucleoside triphosphate+RNA ↔ Thumb
Nucleoside triphosphate + RNA ↔ Pyrophosphate
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SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+RNA+RNA ↔ Thumb+RNA
Adenosine triphosphate + RNA + RNA ↔ Pyrophosphate + RNA
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Thumb+RNA ↔ Thumb+RNA
Guanosine triphosphate + RNA ↔ Pyrophosphate + RNA
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Thumb+RNA ↔ Thumb+RNA
Cytidine triphosphate + RNA ↔ Pyrophosphate + RNA
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Thumb+RNA ↔ Thumb+RNA
Uridine triphosphate + RNA ↔ Pyrophosphate + RNA
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Nucleoside triphosphate+RNA(n) → Thumb+RNA(n+1)
Nucleoside triphosphate + RNA(n) → Pyrophosphate + RNA(n+1)
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Nucleoside triphosphate+RNA ↔ Thumb
Nucleoside triphosphate + RNA ↔ Pyrophosphate
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Complex Reactions:
Nucleoside triphosphate+RNA(n) → Thumb+RNA(n+1)
Nucleoside triphosphate + RNA(n) → Pyrophosphate + RNA(n+1)
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000030Cytidine triphosphateMetaboCard
PAMDB000319Guanosine triphosphateMetaboCard
PAMDB000633PyrophosphateMetaboCard
PAMDB000117Uridine triphosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
DNA binding
DNA-directed RNA polymerase activity
nucleic acid binding
nucleotidyltransferase activity
protein binding
protein dimerization activity
RNA polymerase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
cellular macromolecule biosynthetic process
macromolecule biosynthetic process
metabolic process
transcription
transcription, DNA-dependent
Gene Properties
Locus tag: PA4238
Strand: -
Entrez Gene ID: 881813
Accession: NP_252928.1
GI: 15599434
Sequence start: 4754423
Sequence End: 4755424
Sequence Length: 1001
Gene Sequence: 
>PA4238
ATGCAGAGTTCGGTAAATGAGTTCCTGACCCCCCGCCACATCGATGTGCAGGTGGTCAGTCAAACCCGCGCCAAGATCACGCTCGAGCCTCTCGAGCGTGGTTTTGGTCACACCCTGGGCAACGCGCTGCGTCGCATCCTGTTGTCCTCCATGCCTGGCTGCGCAGTGGTCGAGGCCGAGATCGACGGCGTACTCCACGAGTACTCGGCGATCGAAGGTGTGCAGGAAGATGTAATCGAGATCCTGCTGAACCTGAAAGGTCTGGCCATCAAGCTGCACGGTCGTGATGAAGTGACGCTGACCCTGGCTAAGAAGGGCTCGGGTGTTGTGACTGCTGCCGATATTCAGCTGGATCACGATGTTGAGATCATCAACGGTGACCACGTTATCGCCAACCTGGCAGACAACGGCGCGCTGAACATGAAGCTGAAGGTAGCTCGTGGCCGTGGCTACGAGCCTGCCGACGCACGTCAGAGCGATGAAGACGAAAGCCGCAGCATCGGCCGTCTGCAGCTCGACGCATCGTTCAGCCCGGTCCGTCGTGTCTCCTACGTGGTGGAAAACGCCCGTGTCGAGCAGCGCACCAACCTGGACAAACTGGTCCTGGACCTGGAAACCAACGGCACTCTGGATCCCGAAGAGGCTATCCGTCGCGCCGCTACCATCCTGCAACAGCAGCTGGCAGCGTTCGTGGACCTCAAGGGCGACAGCGAACCCGTCGTTGAAGAGCAGGAAGACGAGATCGATCCGATCCTCCTGCGCCCGGTCGATGACCTGGAACTGACCGTACGTTCGGCCAACTGCCTGAAGGCGGAAAACATCTACTACATCGGTGACCTGATCCAGCGCACCGAAGTGGAACTGTTGAAAACGCCGAACCTGGGCAAGAAGTCCCTGACCGAAATCAAGGACGTTCTGGCTTCCCGTGGTCTGTCCCTCGGTATGCGCCTCGATAACTGGCCGCCGGCAAGTCTTAAGAAAGACGACAAGGCCACTGCCTGA
Protein Properties
Protein Residues: 333
Protein Molecular Weight: 36.6 kDa
Protein Theoretical pI: 4.62
Hydropathicity (GRAVY score): -0.249
Charge at pH 7 (predicted): -15.59
Protein Sequence: 
>PA4238
MQSSVNEFLTPRHIDVQVVSQTRAKITLEPLERGFGHTLGNALRRILLSSMPGCAVVEAEIDGVLHEYSAIEGVQEDVIEILLNLKGLAIKLHGRDEVTLTLAKKGSGVVTAADIQLDHDVEIINGDHVIANLADNGALNMKLKVARGRGYEPADARQSDEDESRSIGRLQLDASFSPVRRVSYVVENARVEQRTNLDKLVLDLETNGTLDPEEAIRRAATILQQQLAAFVDLKGDSEPVVEEQEDEIDPILLRPVDDLELTVRSANCLKAENIYYIGDLIQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLDNWPPASLKKDDKATA
References
External Links:
Resource Link
Genome ID: PA4238
Entrez Gene ID: 881813
NCBI Protein ID: 15599434
General Reference: PaperBLAST - Find papers about PA4238 and its homologs