2-isopropylmalate synthase (PA3792)

Identification
Name: 2-isopropylmalate synthase
Synonyms:
  • Alpha-IPM synthase
  • Alpha-isopropylmalate synthase
Gene Name: leuA
Enzyme Class:
Biological Properties
General Function: Involved in 2-isopropylmalate synthase activity
Specific Function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb
alpha-Ketoisovaleric acid + Acetyl-CoA + Water + a-Ketoisovaleric acid ↔ 2-Isopropylmalic acid + Coenzyme A + Hydrogen ion
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
2-Isopropylmalic acid + Coenzyme A ↔ Acetyl-CoA + alpha-Ketoisovaleric acid + Water
ReactionCard
SMPDB Reactions:
3-Methyl-2-oxovaleric acid+Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
3-Methyl-2-oxovaleric acid + Water + Acetyl-CoA + 3-Methyl-2-oxovaleric acid → Coenzyme A + Hydrogen ion + 2-Isopropylmalic acid
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb+Thumb
alpha-Ketoisovaleric acid + Acetyl-CoA + Water → Hydrogen ion + 3-Carboxy-3-hydroxy-isocaproate + Coenzyme A
ReactionCard
Complex Reactions: Not Available
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0001352-Isopropylmalic acidMetaboCard
PAMDB0005673-Carboxy-3-hydroxy-isocaproateMetaboCard
PAMDB0001413-Methyl-2-oxovaleric acidMetaboCard
PAMDB000004a-Ketoisovaleric acidMetaboCard
PAMDB000298Acetyl-CoAMetaboCard
PAMDB000606alpha-Ketoisovaleric acidMetaboCard
PAMDB000381Coenzyme AMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
2-isopropylmalate synthase activity
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
Process
branched chain family amino acid metabolic process
carboxylic acid metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
leucine biosynthetic process
leucine metabolic process
metabolic process
organic acid metabolic process
oxoacid metabolic process
Gene Properties
Locus tag: PA3792
Strand: +
Entrez Gene ID: 879941
Accession: NP_252481.3
GI: 161486762
Sequence start: 4250828
Sequence End: 4252606
Sequence Length: 1778
Gene Sequence: 
>PA3792
GTGCCTGGTCAGCCCGCGACTGCGTGGCTGAGCGACGCTTCTCTTCATCTCGTCACTGCGTTCGCCTGGCCCCGCCGGCCCAGGAACCGACTCCAAAGGATTAGCCCCATGAGCATGTTGAAAGACCCTTCGCAGAAATACCGCCCATTCTCGGCCATCAATCTGCCCGACCGCACCTGGCCGTCGAAGACCATCACCGAGGTGCCGATCTGGTGCAGTTCCGACCTGCGCGACGGCAACCAGTCGCTGATCGAACCGATGGACGCGGCGAAGAAGATGCGATTCTTCAAGACCCTGGTGCAGGTCGGCCTGAAGCAGATCGAAGTGGCCTTCCCGTCGGCTTCCGATACCGACTTCAACTTCGTCCGCGAGTTGATCGAAGGCAACCACATCCCGGACGACGTCACCATCCAGGTGCTGACCCAGGCCCGCGAAGACCTGATCACCCGCACCTTCGAATCCCTGCGCGGGGCGAAGAAGGCCATCGTCCACGTCTACAACGCCACCGCCCCGTCGTTCCGCCGCATCGTCTTCAACCAGGACAAGCAGGGCGTCGTGGACATCGCCACCAACGCCGCGAAACTGATCAAGAAGCTGGCCGCCGAGCAACCGGAGACCCAGTGGTCGTTCCAGTACTCGCCGGAAATCTTCAGCTCTACCGAGCTGGAGTTCTCGGTGGAGGTCTGCAACGCGGTGATCGACGTCTGGCAACCGACCCCGGACAACAAGATCATCCTCAACCTGCCGGCCACCGTGGAGTGCGCCACGCCGAACGTCTACGCCGACCAGATCGAATGGTTCGGCCGCCACGTCGACAAGCGTGACAGCGTGATCATCAGCCTGCACACCCACAACGACCGTGGCACCGGCGTGGCCGCCACCGAGCTGGGCCTGATGGCCGGCGCCGACCGCGTCGAAGGTTGCCTGTTCGGCAACGGCGAGCGCACCGGCAACGTCGACCTGGTGACCCTGGCCTTGAACATGTACACCCAGGGCCTGCATCCGCAACTGGACTTCTCCGACATCGACGCGGTGCGCAAGGTGGTCGAAGAGTGCAACCAGTTGCCGGTCCACCCGCGCCATCCATATGTCGGCGACCTGGTCCATACCGCTTTCTCCGGCTCGCACCAGGATGCCATTCGCAAGGGCTTCGCCCAGCAAAAGGAAGACGCGATCTGGGAAGTGCCGTACCTGCCGATCGATCCGGCCGACATCGGCCGCGACTACGAAGCGGTGATCCGGGTCAACAGCCAGTCCGGCAAGGGCGGCATCACCTTCCTGCTCGAACAGGAGTACGGCATCAGCCTGCCGCGGCGCATGCAGATCGAGTTCAGCCAGGTAGTGCAAGGCGAGACCGACCGTCTCGGCCTGGAAATGACCGCACAACAGATCTACTCGCTGCTGGAAAACGAATACCTCAAGGCGACCTCGCCCTACGTCCTCGCCAGCCATCGCCTGCAGGAAGAGAACGGCACCAGCGCCGTCGACCTGGAAGTCTCCTTCGACGGCGAGAAGCAGCACTGGCGCGGGATCGGCAAGGGACCGCTGGAAGCCTTGGTGGCCGCCCTGCCGGTCAAGGCGGAAATCATGGACTACCATGAGCACGCCATCGGCGCCGGCGCCAATGCCAAGGCCGCGGCCTATATCGAGATCCGCCTGGAGGGCCAGCGCCCGCTGCACGGCATCGGCATCGACGAGAACATCACCACCGCCAGCTTCCGCGCCCTGTTCAGCGCGCTGAACCGCGCGGTGACCCAGGCCGAGGCCAAGGCGGCCTGA
Protein Properties
Protein Residues: 592
Protein Molecular Weight: 65.8 kDa
Protein Theoretical pI: 5.5
Hydropathicity (GRAVY score): -0.271
Charge at pH 7 (predicted): -13.27
Protein Sequence: 
>PA3792
MPGQPATAWLSDASLHLVTAFAWPRRPRNRLQRISPMSMLKDPSQKYRPFSAINLPDRTWPSKTITEVPIWCSSDLRDGNQSLIEPMDAAKKMRFFKTLVQVGLKQIEVAFPSASDTDFNFVRELIEGNHIPDDVTIQVLTQAREDLITRTFESLRGAKKAIVHVYNATAPSFRRIVFNQDKQGVVDIATNAAKLIKKLAAEQPETQWSFQYSPEIFSSTELEFSVEVCNAVIDVWQPTPDNKIILNLPATVECATPNVYADQIEWFGRHVDKRDSVIISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTLALNMYTQGLHPQLDFSDIDAVRKVVEECNQLPVHPRHPYVGDLVHTAFSGSHQDAIRKGFAQQKEDAIWEVPYLPIDPADIGRDYEAVIRVNSQSGKGGITFLLEQEYGISLPRRMQIEFSQVVQGETDRLGLEMTAQQIYSLLENEYLKATSPYVLASHRLQEENGTSAVDLEVSFDGEKQHWRGIGKGPLEALVAALPVKAEIMDYHEHAIGAGANAKAAAYIEIRLEGQRPLHGIGIDENITTASFRALFSALNRAVTQAEAKAA
References
External Links:
Resource Link
Genome ID: PA3792
Entrez Gene ID: 879941
NCBI Protein ID: 161486762
General Reference: PaperBLAST - Find papers about PA3792 and its homologs