Lysyl-tRNA synthetase (PA3700)

Identification
Name: Lysyl-tRNA synthetase
Synonyms:
  • Lysine--tRNA ligase
  • LysRS
Gene Name: lysS
Enzyme Class:
Biological Properties
General Function: Involved in nucleotide binding
Specific Function: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+tRNA(Lys)+tRNA(Lys) ↔ Thumb+Thumb+L-Lysyl-tRNA+Thumb
Adenosine triphosphate + L-Lysine + tRNA(Lys) + tRNA(Lys) ↔ Adenosine monophosphate + Pyrophosphate + L-Lysyl-tRNA + L-Lysyl-tRNA
ReactionCard
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+tRNA(Lys) → Thumb+L-Lysine-tRNA (Lys)+Thumb
Adenosine triphosphate + L-Lysine + tRNA(Lys) → Adenosine monophosphate + L-Lysine-tRNA (Lys) + Pyrophosphate
ReactionCard
Thumb+Thumb+tRNA(Lys)+tRNA(Lys) ↔ Thumb+Thumb+L-Lysyl-tRNA+Thumb
Adenosine triphosphate + L-Lysine + tRNA(Lys) + tRNA(Lys) ↔ Adenosine monophosphate + Pyrophosphate + L-Lysyl-tRNA + L-Lysyl-tRNA
ReactionCard
Thumb+Thumb+tRNA(Lys) → Thumb+Thumb+L-lysyl-tRNA(Lys)
Adenosine triphosphate + L-Lysine + tRNA(Lys) → Adenosine monophosphate + Pyrophosphate + L-lysyl-tRNA(Lys)
ReactionCard
Thumb+Thumb+tRNA(Lys) → Thumb+Thumb+L-lysyl-tRNA(Lys)
Adenosine triphosphate + L-Lysine + tRNA(Lys) → Adenosine monophosphate + Pyrophosphate + L-lysyl-tRNA(Lys)
ReactionCard
Thumb
L-lysine + a tRNAlys + ATP + H+ → an L-lysyl-[tRNAlys] + AMP + diphosphate
ReactionCard
Complex Reactions:
Thumb+Thumb+tRNA(Lys) → Thumb+L-Lysine-tRNA (Lys)+Thumb
Adenosine triphosphate + L-Lysine + tRNA(Lys) → Adenosine monophosphate + L-Lysine-tRNA (Lys) + Pyrophosphate
ReactionCard
Thumb+Thumb+tRNA(Lys) → Thumb+Thumb+L-lysyl-tRNA(Lys)
Adenosine triphosphate + L-Lysine + tRNA(Lys) → Adenosine monophosphate + Pyrophosphate + L-lysyl-tRNA(Lys)
ReactionCard
Thumb+Thumb+tRNA(Lys) → Thumb+Thumb+L-lysyl-tRNA(Lys)
Adenosine triphosphate + L-Lysine + tRNA(Lys) → Adenosine monophosphate + Pyrophosphate + L-lysyl-tRNA(Lys)
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000075L-LysineMetaboCard
PAMDB004259L-Lysyl-tRNAMetaboCard
PAMDB000633PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
aminoacyl-tRNA ligase activity
ATP binding
binding
catalytic activity
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
ligase activity, forming carbon-oxygen bonds
lysine-tRNA ligase activity
nucleic acid binding
nucleoside binding
nucleotide binding
purine nucleoside binding
Process
biosynthetic process
cellular macromolecule biosynthetic process
cellular macromolecule metabolic process
lysyl-tRNA aminoacylation
macromolecule biosynthetic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
translation
tRNA aminoacylation
tRNA aminoacylation for protein translation
tRNA metabolic process
Gene Properties
Locus tag: PA3700
Strand: -
Entrez Gene ID: 880383
Accession: NP_252390.1
GI: 15598896
Sequence start: 4140884
Sequence End: 4142389
Sequence Length: 1505
Gene Sequence: 
>PA3700
ATGAGCGACCAACAACTCGACCAGCACGAACTGCAACAGGAAGAAAACAAGCTGATCGCCCAGCGCAAGGAAAAACTTGCCGCCGTGCGCGAAGCCCGGGCCATCGCCTTCCCCAACGACTTCCGCCGCGACGCCTACTTCGCGGACCTGCAGAAACAGTACGCCGACAAGACCAAGGAAGAGCTGGAAGCCGCAGCGATCCCGGTCAAGGTGGCCGGTCGGATCATGCTCAACCGCGGTTCCTTCATCGTCCTCCAGGACAGCAGCGAGCGCCTGCAGGTCTACGTCAACCGCAAGACCCTGCCGGAAGAAACCCTGGCCGAGATCAAGACCTGGGACCTGGGCGACATCATCGGCGCCGAAGGCGTGCTGGCCCGCTCGGGCAAGGGCGACCTGTACGTCGACATGACCAGCGTGCGCCTGCTGACCAAGTCGCTGCGTCCGCTGCCGGACAAGCACCATGGCCTGACCGACACCGAGCAGCGCTACCGCCAGCGCTACGTCGACCTGATGGTCAACGAGGAAACCCGCCACACCTTCCGCGTGCGTTCCCAGGTCATCGCCCACATCCGCCGTTTCCTCAGCGAGCGCGGTTTCCTCGAAGTGGAGACCCCGATGCTGCAGACCATCCCCGGCGGCGCCGCGGCCAAGCCGTTCGAGACCCATCACAACGCGCTGGACATGGCCATGTTCCTGCGCATCGCCCCGGAGCTGTACCTCAAGCGCCTGGTGGTCGGCGGTTTCGAGAAGGTCTTCGAGATCAACCGCAACTTCCGTAACGAAGGCGTCTCGACCCGGCACAACCCCGAGTTCACCATGCTCGAGTTCTACCAGGCCTACGCCGACTACGAAGACAACATGGACCTGACCGAGGAACTGTTCCGCGAACTGGCCCAGTCGGTACTCGGTACCACCGACGTGCCCTACGGCGACAAGGTCTTCCACTTCGGCGAGCCGTTCGTGCGCCTGTCGGTGTTCGATTCGATCCTCAAGTACAACCCGGAGATCACCGCCGCCGACCTCAACGACGTCGAGAAGGCGCGGGCCATCGCCAAGAAGGCCGGCGCCAAGGTCCTCGGCCACGAAGGCCTGGGCAAGCTGCAGGTGATGATTTTCGAGGAACTGGTGGAGCACAAGCTGGAGCAGCCGCACTTCATCACCCGCTACCCCTTCGAGGTTTCGCCGCTGGCCCGCCGCAACGACGAAGACCCGAGCGTCACCGACCGCTTCGAGCTGTTCATCGGCGGCCGCGAGATCGCCAATGCCTATTCCGAGCTGAACGACGCCGAGGACCAGGCCGAGCGCTTCATGTTGCAGGTCAAGGAGAAGGACGCCGGCGACGACGAGGCGATGCACTACGACGCCGACTTCATCAATGCCCTGGAATACGGCATGCCGCCGACCGCCGGCGAAGGCATCGGCATCGACCGCCTGGTGATGCTGCTGACCAACTCGCCGTCGATCCGCGACGTGATCCTGTTCCCGCACATGCGCCCGCAGGCCTGA
Protein Properties
Protein Residues: 501
Protein Molecular Weight: 57.3 kDa
Protein Theoretical pI: 5.04
Hydropathicity (GRAVY score): -0.444
Charge at pH 7 (predicted): -17.59
Protein Sequence: 
>PA3700
MSDQQLDQHELQQEENKLIAQRKEKLAAVREARAIAFPNDFRRDAYFADLQKQYADKTKEELEAAAIPVKVAGRIMLNRGSFIVLQDSSERLQVYVNRKTLPEETLAEIKTWDLGDIIGAEGVLARSGKGDLYVDMTSVRLLTKSLRPLPDKHHGLTDTEQRYRQRYVDLMVNEETRHTFRVRSQVIAHIRRFLSERGFLEVETPMLQTIPGGAAAKPFETHHNALDMAMFLRIAPELYLKRLVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEELFRELAQSVLGTTDVPYGDKVFHFGEPFVRLSVFDSILKYNPEITAADLNDVEKARAIAKKAGAKVLGHEGLGKLQVMIFEELVEHKLEQPHFITRYPFEVSPLARRNDEDPSVTDRFELFIGGREIANAYSELNDAEDQAERFMLQVKEKDAGDDEAMHYDADFINALEYGMPPTAGEGIGIDRLVMLLTNSPSIRDVILFPHMRPQA
References
External Links:
Resource Link
Genome ID: PA3700
Entrez Gene ID: 880383
NCBI Protein ID: 15598896
General Reference: PaperBLAST - Find papers about PA3700 and its homologs