P. aeruginosa Metabolome Database Protein PA2856

Identification

Name:

Acyl-CoA thioesterase I

Synonyms:

Lecithinase B
Lysophospholipase L1
Protease I

Gene Name:

tesA

Enzyme Class:

3.1.1.5

Biological Properties

General Function:

Involved in hydrolase activity

Specific Function:

Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin

Cellular Location:

Periplasm

KEGG Pathways:

Biosynthesis of unsaturated fatty acids pae01040
Fatty acid metabolism pae00071
Glycerophospholipid metabolism pae00564

KEGG Reactions:

1-Acyl-sn-glycero-3-phosphocholine

↔

1-Acyl-sn-glycero-3-phosphocholine + Water ↔ Glycerophosphocholine + Carboxylate
ReactionCard

SMPDB Reactions:

a palmitoleoyl-[acp]?

→

a holo-[acyl-carrier protein]

Hexadecenoate (n-C16:1)

Water + a palmitoleoyl-[acp]? → a holo-[acyl-carrier protein] + Hydrogen ion + Hexadecenoate (n-C16:1)
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a cis-vaccenoyl-[acp]

→

a holo-[acyl-carrier protein]

a cis-vaccenoyl-[acp] + Water → Hydrogen ion + a holo-[acyl-carrier protein] + Vaccenic acid
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PseudoCyc/BioCyc Reactions:

→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C12:0) + Water → Dodecanoate (N-C12:0) + Glycerylphosphorylethanolamine + Hydrogen ion
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→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C14:0) + Water → Glycerylphosphorylethanolamine + Hydrogen ion + tetradecanoate (n-C14:0)
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→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C14:1) + Water → Glycerylphosphorylethanolamine + Hydrogen ion + Tetradecenoate (N-C14:1)
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→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C16:0) + Water → Glycerylphosphorylethanolamine + Hydrogen ion + Palmitic acid
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→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C18:0) + Water → Glycerylphosphorylethanolamine + Hydrogen ion + Octadecanoate (N-C18:0)
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→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C18:1) + Water → Glycerylphosphorylethanolamine + Hydrogen ion + Octadecenoate (N-C18:1)
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→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C12:0) + Water → Dodecanoate (N-C12:0) + Glycerophosphoglycerol + Hydrogen ion
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→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C14:0) + Water → Glycerophosphoglycerol + Hydrogen ion + tetradecanoate (n-C14:0)
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→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C14:1) + Water → Glycerophosphoglycerol + Hydrogen ion + Tetradecenoate (N-C14:1)
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→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C16:0) + Water → Glycerophosphoglycerol + Hydrogen ion + Palmitic acid
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→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C18:0) + Water → Glycerophosphoglycerol + Hydrogen ion + Octadecanoate (N-C18:0)
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→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C18:1) + Water → Glycerophosphoglycerol + Hydrogen ion + Octadecenoate (N-C18:1)
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→

1-Dodecanoyl-sn-glycerol 3-phosphate + Water → Dodecanoate (N-C12:0) + Glycerol 3-phosphate + Hydrogen ion
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→

1-hexadecanoyl-sn-glycerol 3-phosphate + Water → Glycerol 3-phosphate + Hydrogen ion + Palmitic acid
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→

1-Octadec-11-enoyl-sn-glycerol 3-phosphate + Water → Glycerol 3-phosphate + Hydrogen ion + Octadecenoate (N-C18:1)
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→

1-Octadecanoyl-sn-glycerol 3-phosphate + Water → Glycerol 3-phosphate + Hydrogen ion + Octadecanoate (N-C18:0)
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→

1-Tetradec-7-enoyl-sn-glycerol 3-phosphate + Water → Glycerol 3-phosphate + Hydrogen ion + Tetradecenoate (N-C14:1)
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→

1-Tetradecanoyl-sn-glycerol 3-phosphate + Water → Glycerol 3-phosphate + Hydrogen ion + tetradecanoate (n-C14:0)
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1-Acyl-sn-glycero-3-phosphocholine

↔

1-Acyl-sn-glycero-3-phosphocholine + Water ↔ Glycerophosphocholine + Carboxylate
ReactionCard

a palmitoleoyl-[acp]?

→

a holo-[acyl-carrier protein]

Hexadecenoate (n-C16:1)

Water + a palmitoleoyl-[acp]? → a holo-[acyl-carrier protein] + Hydrogen ion + Hexadecenoate (n-C16:1)
ReactionCard

a cis-vaccenoyl-[acp]

→

a holo-[acyl-carrier protein]

a cis-vaccenoyl-[acp] + Water → Hydrogen ion + a holo-[acyl-carrier protein] + Vaccenic acid
ReactionCard

→

eicosapentaenoyl-CoA + Water → eicosapentaenoate + coenzyme A + Hydrogen ion
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→

3-trans,5-cis-tetradecadienoyl-CoA + Water → Hydrogen ion + (3E,5Z)-tetradecadienoate + coenzyme A
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→

palmitoyl-CoA + Water → Hydrogen ion + palmitate + coenzyme A
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→

jasmonoyl-CoA + Water → Hydrogen ion + (+)-7-iso-jasmonate + coenzyme A
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→

dihomo γ-linolenoyl-CoA + Water → di-homo-γ-linolenate + coenzyme A + Hydrogen ion
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ACYL-COA

→

Carboxylates

ACYL-COA + Water → coenzyme A + Carboxylates + Hydrogen ion
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→

stearoyl-CoA + Water → Hydrogen ion + Octadecanoate (N-C18:0) + coenzyme A
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Complex Reactions:

→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C12:0) + Water → Dodecanoate (N-C12:0) + Glycerylphosphorylethanolamine + Hydrogen ion
ReactionCard

→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C14:0) + Water → Glycerylphosphorylethanolamine + Hydrogen ion + tetradecanoate (n-C14:0)
ReactionCard

→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C14:1) + Water → Glycerylphosphorylethanolamine + Hydrogen ion + Tetradecenoate (N-C14:1)
ReactionCard

→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C16:0) + Water → Glycerylphosphorylethanolamine + Hydrogen ion + Palmitic acid
ReactionCard

→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C18:0) + Water → Glycerylphosphorylethanolamine + Hydrogen ion + Octadecanoate (N-C18:0)
ReactionCard

→

1-Acyl-sn-glycero-3-phosphoethanolamine (N-C18:1) + Water → Glycerylphosphorylethanolamine + Hydrogen ion + Octadecenoate (N-C18:1)
ReactionCard

→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C12:0) + Water → Dodecanoate (N-C12:0) + Glycerophosphoglycerol + Hydrogen ion
ReactionCard

→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C14:0) + Water → Glycerophosphoglycerol + Hydrogen ion + tetradecanoate (n-C14:0)
ReactionCard

→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C14:1) + Water → Glycerophosphoglycerol + Hydrogen ion + Tetradecenoate (N-C14:1)
ReactionCard

→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C16:0) + Water → Glycerophosphoglycerol + Hydrogen ion + Palmitic acid
ReactionCard

→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C18:0) + Water → Glycerophosphoglycerol + Hydrogen ion + Octadecanoate (N-C18:0)
ReactionCard

→

1-Acyl-sn-glycero-3-phosphoglycerol (N-C18:1) + Water → Glycerophosphoglycerol + Hydrogen ion + Octadecenoate (N-C18:1)
ReactionCard

→

1-Dodecanoyl-sn-glycerol 3-phosphate + Water → Dodecanoate (N-C12:0) + Glycerol 3-phosphate + Hydrogen ion
ReactionCard

→

1-hexadecanoyl-sn-glycerol 3-phosphate + Water → Glycerol 3-phosphate + Hydrogen ion + Palmitic acid
ReactionCard

→

1-Octadec-11-enoyl-sn-glycerol 3-phosphate + Water → Glycerol 3-phosphate + Hydrogen ion + Octadecenoate (N-C18:1)
ReactionCard

→

1-Octadecanoyl-sn-glycerol 3-phosphate + Water → Glycerol 3-phosphate + Hydrogen ion + Octadecanoate (N-C18:0)
ReactionCard

→

1-Tetradec-7-enoyl-sn-glycerol 3-phosphate + Water → Glycerol 3-phosphate + Hydrogen ion + Tetradecenoate (N-C14:1)
ReactionCard

→

1-Tetradecanoyl-sn-glycerol 3-phosphate + Water → Glycerol 3-phosphate + Hydrogen ion + tetradecanoate (n-C14:0)
ReactionCard

Transports:

Not Available

Metabolites:

PAMDB ID	Name	View
PAMDB001522	1-Acyl-sn-glycero-3-phosphoethanolamine (N-C12:0)	MetaboCard
PAMDB001523	1-Acyl-sn-glycero-3-phosphoethanolamine (N-C14:0)	MetaboCard
PAMDB001524	1-Acyl-sn-glycero-3-phosphoethanolamine (N-C14:1)	MetaboCard
PAMDB001525	1-Acyl-sn-glycero-3-phosphoethanolamine (N-C16:0)	MetaboCard
PAMDB001526	1-Acyl-sn-glycero-3-phosphoethanolamine (N-C16:1)	MetaboCard
PAMDB001527	1-Acyl-sn-glycero-3-phosphoethanolamine (N-C18:0)	MetaboCard
PAMDB001528	1-Acyl-sn-glycero-3-phosphoethanolamine (N-C18:1)	MetaboCard
PAMDB001529	1-Acyl-sn-glycero-3-phosphoglycerol (N-C12:0)	MetaboCard
PAMDB001530	1-Acyl-sn-glycero-3-phosphoglycerol (N-C14:0)	MetaboCard
PAMDB001531	1-Acyl-sn-glycero-3-phosphoglycerol (N-C14:1)	MetaboCard
PAMDB001532	1-Acyl-sn-glycero-3-phosphoglycerol (N-C16:0)	MetaboCard
PAMDB001533	1-Acyl-sn-glycero-3-phosphoglycerol (N-C16:1)	MetaboCard
PAMDB001534	1-Acyl-sn-glycero-3-phosphoglycerol (N-C18:0)	MetaboCard
PAMDB001535	1-Acyl-sn-glycero-3-phosphoglycerol (N-C18:1)	MetaboCard
PAMDB001536	1-Dodecanoyl-sn-glycerol 3-phosphate	MetaboCard
PAMDB001537	1-Hexadec-9-enoyl-sn-glycerol 3-phosphate	MetaboCard
PAMDB001538	1-hexadecanoyl-sn-glycerol 3-phosphate	MetaboCard
PAMDB001539	1-Octadec-11-enoyl-sn-glycerol 3-phosphate	MetaboCard
PAMDB001540	1-Octadecanoyl-sn-glycerol 3-phosphate	MetaboCard
PAMDB001541	1-Tetradec-7-enoyl-sn-glycerol 3-phosphate	MetaboCard
PAMDB001542	1-Tetradecanoyl-sn-glycerol 3-phosphate	MetaboCard
PAMDB001617	Dodecanoate (N-C12:0)	MetaboCard
PAMDB000049	Glycerol 3-phosphate	MetaboCard
PAMDB000032	Glycerophosphocholine	MetaboCard
PAMDB001631	Glycerophosphoglycerol	MetaboCard
PAMDB000042	Glycerylphosphorylethanolamine	MetaboCard
PAMDB001633	Hydrogen ion	MetaboCard
PAMDB001745	Octadecanoate (N-C18:0)	MetaboCard
PAMDB001746	Octadecenoate (N-C18:1)	MetaboCard
PAMDB000091	Palmitic acid	MetaboCard
PAMDB001757	tetradecanoate (n-C14:0)	MetaboCard
PAMDB001758	Tetradecenoate (N-C14:1)	MetaboCard
PAMDB001838	Vaccenic acid	MetaboCard
PAMDB000142	Water	MetaboCard

GO Classification:

Function
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
lipase activity
Process
lipid metabolic process
metabolic process
primary metabolic process

Gene Properties

Locus tag:

PA2856

Strand:

Entrez Gene ID:

878554

Accession:

NP_251546.1

GI:

15598052

Sequence start:

3208673

Sequence End:

3209278

Sequence Length:

605

Gene Sequence:

>PA2856
ATGCGTGCATTGCTGTTGAGCGGCTGCCTGGCCCTGGTCCTGCTGACCCAGCAGGCCGCGGCGCAGACGCTGTTGGTCGTGGGCGATAGTATCAGCGCCGCTTTGGGACTGGATACCAGCCAGGGCTGGGTCGCCCTGCTGCAGAAGCGCCTCGCCGACGAAGGTTACGACTACCGGGTGGTGAACGCCTCGATCAGCGGCGACACCTCCGCGGGTGGCCTGGCGCGCCTGCCGGCGCTGCTTGCCGAGGAGAAGCCGGCGCTGGTGGTGATCGAGCTGGGAGGCAACGACGGGCTGCGCGGAATGGCACCGGCGCAATTGCAACAGAATCTTGCCAGCATGGCGCAAAAGGCCCGGGCGGAAGGAGCGAAGGTGCTGCTGCTGGGTATCCAGTTGCCACCGAACTACGGCCCGCGCTACATCGAGGCCTTTTCCAGGGTCTACGGCGCGGTCGCGGCGCAGGAAAAGACCGCCCTGGTGCCGTTTTTCCTCGAAGGGGTCGGCGGGGTCCAGGGGATGATGCAGGCGGATGGCATCCATCCGGCGCTCGCCGCCCAGCCGAGGCTCCTGGAAAATGTCTGGCCGACGCTGAAACCGCTGCTTTGA

Protein Properties

Protein Residues:

201

Protein Molecular Weight:

21 kDa

Protein Theoretical pI:

5.91

Hydropathicity (GRAVY score):

0.318

Charge at pH 7 (predicted):

-0.8

Protein Sequence:

>PA2856
MRALLLSGCLALVLLTQQAAAQTLLVVGDSISAALGLDTSQGWVALLQKRLADEGYDYRVVNASISGDTSAGGLARLPALLAEEKPALVVIELGGNDGLRGMAPAQLQQNLASMAQKARAEGAKVLLLGIQLPPNYGPRYIEAFSRVYGAVAAQEKTALVPFFLEGVGGVQGMMQADGIHPALAAQPRLLENVWPTLKPLL

References

External Links:

Resource	Link
Genome ID:	PA2856
Entrez Gene ID:	878554
NCBI Protein ID:	15598052

General Reference:

PaperBLAST - Find papers about PA2856 and its homologs

Acyl-CoA thioesterase I (PA2856)