Cysteinyl-tRNA synthetase (PA1795)

Identification
Name: Cysteinyl-tRNA synthetase
Synonyms:
  • Cysteine--tRNA ligase
  • CysRS
Gene Name: cysS
Enzyme Class:
Biological Properties
General Function: Involved in cysteine-tRNA ligase activity
Specific Function: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+tRNA(Cys)+tRNA(Cys) ↔ Thumb+L-Cysteinyl-tRNA(Cys)+Thumb+L-Cysteinyl-tRNA(Cys)
Adenosine triphosphate + L-Cysteine + tRNA(Cys) + tRNA(Cys) ↔ Adenosine monophosphate + L-Cysteinyl-tRNA(Cys) + Pyrophosphate + L-Cysteinyl-tRNA(Cys)
ReactionCard
Thumb+Thumb+tRNA(Cys) ↔ Thumb+Thumb+L-Cysteinyl-tRNA(Cys)
Adenosine triphosphate + L-Cysteine + tRNA(Cys) ↔ Adenosine monophosphate + Pyrophosphate + L-Cysteinyl-tRNA(Cys)
ReactionCard
SMPDB Reactions:
Thumb+tRNA(Cys)+Thumb+Thumb → Pyrophosphate+Thumb+L-cysteinyl-tRNA(Cys)
L-Cysteine + tRNA(Cys) + Adenosine triphosphate + Hydrogen ion → Pyrophosphate + Adenosine monophosphate + L-cysteinyl-tRNA(Cys)
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+tRNA(Cys)+tRNA(Cys) ↔ Thumb+L-Cysteinyl-tRNA(Cys)+Thumb+L-Cysteinyl-tRNA(Cys)
Adenosine triphosphate + L-Cysteine + tRNA(Cys) + tRNA(Cys) ↔ Adenosine monophosphate + L-Cysteinyl-tRNA(Cys) + Pyrophosphate + L-Cysteinyl-tRNA(Cys)
ReactionCard
Thumb+Thumb+tRNA(Cys) ↔ Thumb+Thumb+L-Cysteinyl-tRNA(Cys)
Adenosine triphosphate + L-Cysteine + tRNA(Cys) ↔ Adenosine monophosphate + Pyrophosphate + L-Cysteinyl-tRNA(Cys)
ReactionCard
Thumb+Thumb+tRNA(Cys) → Thumb+Thumb+L-cysteinyl-tRNA(Cys)
Adenosine triphosphate + L-Cysteine + tRNA(Cys) → Adenosine monophosphate + Pyrophosphate + L-cysteinyl-tRNA(Cys)
ReactionCard
Thumb+tRNA(Cys)+Thumb+Thumb → Pyrophosphate+Thumb+L-cysteinyl-tRNA(Cys)
L-Cysteine + tRNA(Cys) + Adenosine triphosphate + Hydrogen ion → Pyrophosphate + Adenosine monophosphate + L-cysteinyl-tRNA(Cys)
ReactionCard
Thumb
a tRNAcys + L-cysteine + ATP + H+ → an L-cysteinyl-[tRNAcys] + AMP + diphosphate
ReactionCard
Complex Reactions:
Thumb+Thumb+tRNA(Cys) → Thumb+Thumb+L-cysteinyl-tRNA(Cys)
Adenosine triphosphate + L-Cysteine + tRNA(Cys) → Adenosine monophosphate + Pyrophosphate + L-cysteinyl-tRNA(Cys)
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000152L-CysteineMetaboCard
PAMDB000633PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
aminoacyl-tRNA ligase activity
ATP binding
binding
catalytic activity
cysteine-tRNA ligase activity
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
ligase activity, forming carbon-oxygen bonds
nucleoside binding
nucleotide binding
purine nucleoside binding
Process
biosynthetic process
cellular macromolecule biosynthetic process
cellular macromolecule metabolic process
cysteinyl-tRNA aminoacylation
macromolecule biosynthetic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
translation
tRNA aminoacylation
tRNA aminoacylation for protein translation
tRNA metabolic process
Gene Properties
Locus tag: PA1795
Strand: +
Entrez Gene ID: 880609
Accession: NP_250486.1
GI: 15596992
Sequence start: 1944747
Sequence End: 1946129
Sequence Length: 1382
Gene Sequence: 
>PA1795
GTGCTGCAGATCTACAACACGCTGAGCAAGACCAAAGAAGTCTTCACGCCGCTGGTCGGCAACCAGGTGCGCATGTACGTGTGCGGCATGACCGTGTACGACTACTGCCATCTGGGCCACGGCCGCAGCATGGTGGCGTTCGACGTGATCACCCGCTGGTTGCGCCACCGTGGCTACGACCTGACGTACGTGCGCAACATCACCGATATCGATGACAAGATCATCAATCGCGCCAACGAGAACGGCGAACCGTTCGATGTGCTGACCGAGCGCATGATCGCGGCGATGCACGAGGACGAGGCCCGGCTGAACATCCTCAAGCCGGACCAGGAGCCGCGTGCCACCGATCACATCGCCGGCATGCACGCGATGATCCAGACCCTGATCGACAAGGGCTATGCCTACGCGCCGGGCAACGGTGACGTGTACTACCGGGTCGGCAAGTTCGCTGGCTACGGCAAGTTGTCGCGGCGGCGGGTCGAGGACCTGCGCATCGGTGCGCGGATCGAACCGGGCGAAGCGAAGGAGGATCCGCTCGATTTCGTGCTCTGGAAGGGCGCCAAGCCGGGCGAGCCGAGCTGGTCGTCGCCCTGGGGCGAGGGGCGCCCGGGCTGGCACATCGAATGCTCGGTGATGTCCACCTGCTGCCTGGGCGATTCCTTCGACATCCATGGCGGTGGCAACGACCTGGAGTTCCCTCACCACGAGAACGAGATCGCCCAGAGCGAGGCCGCGACCGGCAAGCCCTACGCCAAGTCCTGGCTGCACTGCGGGATGATCACCATCAACGGCGAGAAGATGTCCAAGTCGCTGGGCAATTTCTTCACCATCCGCGAAGTGCTGGAGAAATATCACCCCGAAGTGGTGCGTTACCTGCTGATCGCCAGCCATTACCGCAGTCCGATCAACTACTCGGAGGAGAACCTGCGCGAGGCCAAGGCGGCCCTGGACCGTTTCTACAACGCGCTCAAGGGGCTGCCCGAGGCGGCGCCGGCGGAAGCGGCGGAGTATGTCGAGCGCTTCGCCGCGGCGATGGACGACGACTTCAATACCGCGGGTGCCTGCTCGGTGCTGTTCGAGCTGGCGCGGGAGGTCAATCGTCTGCGCGAAAGCGACCTGTCGGCGGCGGCCGCGCTGGCCGCGCGCCTGAAGCAGTTGGCTGGCCTGCTGGGGGTGCTGCAACTGGAGCCGGAAGCCTTCCTGCAGGCGGGCGCCGAGGGCAAGGTCGATGCGGCCGAGGTCGAGGCGCTGATCCAGGCGCGCCTGGAGGCGCGGGCGGCGAAGAACTGGGCGGAGTCCGACCGGATTCGCGATCAGCTCACCGCCATGGGCGTAGTGCTGGAGGATGGCAAGGGCGGCACTACCTGGCGCCTGGCCGACTGA
Protein Properties
Protein Residues: 460
Protein Molecular Weight: 51.2 kDa
Protein Theoretical pI: 5.38
Hydropathicity (GRAVY score): -0.343
Charge at pH 7 (predicted): -12.06
Protein Sequence: 
>PA1795
MLQIYNTLSKTKEVFTPLVGNQVRMYVCGMTVYDYCHLGHGRSMVAFDVITRWLRHRGYDLTYVRNITDIDDKIINRANENGEPFDVLTERMIAAMHEDEARLNILKPDQEPRATDHIAGMHAMIQTLIDKGYAYAPGNGDVYYRVGKFAGYGKLSRRRVEDLRIGARIEPGEAKEDPLDFVLWKGAKPGEPSWSSPWGEGRPGWHIECSVMSTCCLGDSFDIHGGGNDLEFPHHENEIAQSEAATGKPYAKSWLHCGMITINGEKMSKSLGNFFTIREVLEKYHPEVVRYLLIASHYRSPINYSEENLREAKAALDRFYNALKGLPEAAPAEAAEYVERFAAAMDDDFNTAGACSVLFELAREVNRLRESDLSAAAALAARLKQLAGLLGVLQLEPEAFLQAGAEGKVDAAEVEALIQARLEARAAKNWAESDRIRDQLTAMGVVLEDGKGGTTWRLAD
References
External Links:
Resource Link
Genome ID: PA1795
Entrez Gene ID: 880609
NCBI Protein ID: 15596992
General Reference: PaperBLAST - Find papers about PA1795 and its homologs