2-methylcitrate synthase (PA0795)

Identification
Name: 2-methylcitrate synthase
Synonyms:
  • Citrate synthase 2
  • Methylcitrate synthase
Gene Name: prpC
Enzyme Class:
Biological Properties
General Function: Involved in transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
Specific Function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
Water + Oxalacetic acid + Propionyl-CoA ↔ Methylcitric acid + Coenzyme A + Hydrogen ion + (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
Methylcitric acid + Coenzyme A ↔ Propionyl-CoA + Oxalacetic acid + Water
ReactionCard
SMPDB Reactions:
Propionyl-CoA+Thumb+Thumb+Thumb → Thumb+Thumb+2-Methylcitric acid+Thumb
Propionyl-CoA + Water + Oxalacetic acid + Propionyl-CoA → Coenzyme A + Hydrogen ion + 2-Methylcitric acid + Methylcitric acid
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
Water + Oxalacetic acid + Propionyl-CoA ↔ Methylcitric acid + Coenzyme A + Hydrogen ion + (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
ReactionCard
Complex Reactions: Not Available
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB003442(2R,3S)-2-Hydroxybutane-1,2,3-tricarboxylateMetaboCard
PAMDB000381Coenzyme AMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000178Methylcitric acidMetaboCard
PAMDB000092Oxalacetic acidMetaboCard
PAMDB000321Propionyl-CoAMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
Process
acetyl-CoA catabolic process
acetyl-CoA metabolic process
carbohydrate metabolic process
cellular carbohydrate metabolic process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
primary metabolic process
tricarboxylic acid cycle
Gene Properties
Locus tag: PA0795
Strand: -
Entrez Gene ID: 880674
Accession: NP_249486.1
GI: 15595992
Sequence start: 872986
Sequence End: 874113
Sequence Length: 1127
Gene Sequence: 
>PA0795
ATGGCTGAAGCAAAAGTACTGAGTGGCGCCGGCCTGCGTGGCCAGGTGGCCGGTCAGACAGCCCTGTCCACCGTCGGCCAGGAAGGCGCCGGCCTGACCTACCGTGGCTACGACGTCCGCGACCTGGCGGCGGCGGCGATCTTCGAGGAAGTCGCCTACCTGCTGCTCTACGGCGAACTGCCGAACAAGCAACAGCTCGACGCCTACCTGAAGAAGCTCCAGGGCCAGCGCGACCTGCCGCAGGCGCTGAAGGAAGTTCTCGAACGGATTCCCAAGGACGCCCACCCGATGGACGTGATGCGCACTGGCGCCTCGGTCCTCGGCACCCTGGAACCGGAGCTGAGCTTCGACCAGCAGCGCGACGTCGCCGACCGCCTGCTCGCCGCCTTCCCGGCGATCATGACCTACTGGTACCGCTTCACCCACGAAGGCCAGCGCATCGACTGCAACAGCGACGAGCCGACCATCGGCGGGCACTTCCTCGCCCTGCTCCACGGCAAGAAGCCCAGCGAGCTGCACGTGAAGGTGATGAACGTCTCGCTGATCCTCTACGCCGAGCATGAGTTCAACGCATCCACCTTCACCGCGCGGGTTTGCGCCTCGACCCTGTCCGACCTCTACTCCTGCGTCACCGGCGCCATCGGCTCGCTGCGCGGCCCGCTGCACGGCGGCGCCAACGAGGCGGCGATGGAACTGATCGAACGCTTCTCTTCGCCGCAGGAAGCCACCGCCGAGCTGCTGAAGATGCTCGAGCGCAAGGACAAGATCATGGGCTTCGGGCATGCCATCTACAAGGATTCCGACCCACGCAACGAAGTGATCAAGGGTTGGTCGAAGCAGCTCGCCGACGAGGTCGGCGACAAGGTCCTGTTCGCGGTTTCCGAGGCCATCGACAAGACCATGTGGGAGCAGAAGAAGCTGTTCCCCAACGCCGACTTCTACCACGCCTCGGCGTATCACTTCATGGGCATCCCGACCAAGCTGTTCACGCCGATCTTCGTCTGCTCGCGGACCAGCGGCTGGACCGCCCACGTCTTCGAACAGCGCGCCAACAACCGCATCATCCGCCCGAGCGCCGAGTACACCGGCGTCGAACAGCGTGCGTTCGTGCCGCTGGAGCAACGCTGA
Protein Properties
Protein Residues: 375
Protein Molecular Weight: 41.7 kDa
Protein Theoretical pI: 6.44
Hydropathicity (GRAVY score): -0.234
Charge at pH 7 (predicted): -3.47
Protein Sequence: 
>PA0795
MAEAKVLSGAGLRGQVAGQTALSTVGQEGAGLTYRGYDVRDLAAAAIFEEVAYLLLYGELPNKQQLDAYLKKLQGQRDLPQALKEVLERIPKDAHPMDVMRTGASVLGTLEPELSFDQQRDVADRLLAAFPAIMTYWYRFTHEGQRIDCNSDEPTIGGHFLALLHGKKPSELHVKVMNVSLILYAEHEFNASTFTARVCASTLSDLYSCVTGAIGSLRGPLHGGANEAAMELIERFSSPQEATAELLKMLERKDKIMGFGHAIYKDSDPRNEVIKGWSKQLADEVGDKVLFAVSEAIDKTMWEQKKLFPNADFYHASAYHFMGIPTKLFTPIFVCSRTSGWTAHVFEQRANNRIIRPSAEYTGVEQRAFVPLEQR
References
External Links:
Resource Link
Genome ID: PA0795
Entrez Gene ID: 880674
NCBI Protein ID: 15595992
General Reference: PaperBLAST - Find papers about PA0795 and its homologs