Identification
Name: S-adenosylmethionine synthase
Synonyms:
  • AdoMet synthase
  • MAT
  • Methionine adenosyltransferase
Gene Name: metK
Enzyme Class:
Biological Properties
General Function: Involved in methionine adenosyltransferase activity
Specific Function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. Is essential for growth
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Thumb+Thumb+ThumbThumb+Thumb+Thumb
SMPDB Reactions:
Thumb+Thumb+ThumbThumb+Pyrophosphate
Thumb+Thumb+ThumbThumb+Pyrophosphate+S-adenosyl-L-methionine
L-Methionine + Water + Adenosine triphosphatePhosphate + Pyrophosphate + S-adenosyl-L-methionine
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+ThumbThumb+Thumb+Thumb
Complex Reactions: Not Available
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB001776Inorganic phosphateMetaboCard
PAMDB000175L-MethionineMetaboCard
PAMDB000383PhosphateMetaboCard
PAMDB000633PyrophosphateMetaboCard
PAMDB000289S-AdenosylmethionineMetaboCard
PAMDB000808Se-AdenosylselenomethionineMetaboCard
PAMDB000496TriphosphateMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
methionine adenosyltransferase activity
nucleoside binding
purine nucleoside binding
transferase activity
transferase activity, transferring alkyl or aryl (other than methyl) groups
Process
cellular metabolic process
metabolic process
one-carbon metabolic process
Gene Properties
Locus tag: PA0546
Strand: -
Entrez Gene ID: 878379
Accession: NP_249237.1
GI: 15595743
Sequence start: 603706
Sequence End: 604896
Sequence Length: 1190
Gene Sequence:
>PA0546
ATGAGCGAATATTCCGTTTTCACCTCCGAGTCCGTGTCCGAAGGCCATCCGGACAAGATCGCGGACCAGATCTCCGACGCGGTGCTGGACGCCATCATCGCCAAGGACAAGTACGCTCGCGTGGCATGCGAAACCCTGGTCAAGACCGGCGTGGCGATCATCGCCGGTGAAGTCACCACCTCCGCCTGGGTCGACCTGGAAGAGCTGGTGCGCAAGGTCATCATCGACATCGGCTACGACAGCTCCGACGTCGGCTTCGATGGTGCCACCTGCGGCGTGCTGAACATCATCGGCAAGCAGTCGGTGGACATCAACCAGGGCGTCGACCGGGCCAAGCCGGAAGACCAGGGCGCCGGCGACCAGGGCCTGATGTTCGGCTACGCCAGCAACGAGACCGACGTGCTGATGCCGGCGCCGATCTGTTTCTCCCACCGCCTGGTCGAGCGCCAGGCCGAGGCGCGCAAGTCCGGCCTGCTGCCATGGCTGCGCCCGGACGCCAAGTCCCAGGTGACCTGCCGCTACGAAGGCGGCAAGGTGGTCGGCATCGATGCGGTGGTGCTGTCCACCCAGCACAACCCGGAAGTCTCCTATAACGACCTGCGCGACGGCGTGATGGAGCTGATCATCAAGCAGGTGCTGCCGGCCGAGCTGCTGCACAAGGACACCCAGTTCCACATCAACCCGACCGGCAACTTCGTGATCGGCGGCCCGGTCGGCGACTGCGGCCTGACCGGACGCAAGATCATCGTCGACTCCTACGGCGGCATGGCCCGCCACGGCGGCGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGCTCCGCCGCCTACGCCGGCCGCTACGTGGCGAAGAACATCGTCGCCGCCGGCCTCGCCGAGCGTTGCGAGATCCAGGTTTCCTACGCCATCGGCGTGGCCCAGCCGACCTCGATCTCGATCAACACCTTCGGCACCGGCAAGGTCAGCGACGAGAAGATCGTGCAACTGGTCCGCGAGCACTTCGACCTGCGCCCGTACGCGATCACCAAGATGCTCGACCTGCTCCACCCGATGTACCAGCCGACCGCGGCCTACGGCCACTTCGGTCGTCATCCGTTCGAGCTGACCGTGGACGGCGATACCTTCACCGCGTTCACCTGGGAGAAGACCGACAAGGCCGCTCTCCTGCGCGACGCCGCCGGCCTCTGA
Protein Properties
Protein Residues: 396
Protein Molecular Weight: 42.7 kDa
Protein Theoretical pI: 5.15
Hydropathicity (GRAVY score): -0.103
Charge at pH 7 (predicted): -10.77
Protein Sequence:
>PA0546
MSEYSVFTSESVSEGHPDKIADQISDAVLDAIIAKDKYARVACETLVKTGVAIIAGEVTTSAWVDLEELVRKVIIDIGYDSSDVGFDGATCGVLNIIGKQSVDINQGVDRAKPEDQGAGDQGLMFGYASNETDVLMPAPICFSHRLVERQAEARKSGLLPWLRPDAKSQVTCRYEGGKVVGIDAVVLSTQHNPEVSYNDLRDGVMELIIKQVLPAELLHKDTQFHINPTGNFVIGGPVGDCGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQVSYAIGVAQPTSISINTFGTGKVSDEKIVQLVREHFDLRPYAITKMLDLLHPMYQPTAAYGHFGRHPFELTVDGDTFTAFTWEKTDKAALLRDAAGL
References
External Links:
Resource Link
Genome ID: PA0546
Entrez Gene ID: 878379
NCBI Protein ID: 15595743
General Reference: PaperBLAST - Find papers about PA0546 and its homologs