Identification
Name: Leucyl-tRNA synthetase
Synonyms:
  • Leucine--tRNA ligase
  • LeuRS
Gene Name: leuS
Enzyme Class:
Biological Properties
General Function: Involved in nucleotide binding
Specific Function: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Cellular Location: Cytoplasm
KEGG Pathways:
  • Aminoacyl-tRNA biosynthesis pae00970
  • Valine, leucine and isoleucine biosynthesis pae00290
KEGG Reactions:
Thumb+Thumb+tRNA(Leu)+tRNA(Leu)Thumb+Thumb+L-Leucyl-tRNA+L-Leucyl-tRNA
Adenosine triphosphate + L-Leucine + tRNA(Leu) + tRNA(Leu) ↔ Adenosine monophosphate + Pyrophosphate + L-Leucyl-tRNA + L-Leucyl-tRNA
ReactionCard
SMPDB Reactions:
Thumb+Thumb+Thumb+tRNA(Leu)Thumb+Pyrophosphate+L-Leucyl-tRNA(Leu)
L-Leucine + Adenosine triphosphate + Hydrogen ion + tRNA(Leu) → Adenosine monophosphate + Pyrophosphate + L-Leucyl-tRNA(Leu)
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+tRNA(Leu)Thumb+L-Leucyl-tRNA(Leu)+Thumb
Thumb+Thumb+tRNA(Leu)+tRNA(Leu)Thumb+Thumb+L-Leucyl-tRNA+L-Leucyl-tRNA
Adenosine triphosphate + L-Leucine + tRNA(Leu) + tRNA(Leu) ↔ Adenosine monophosphate + Pyrophosphate + L-Leucyl-tRNA + L-Leucyl-tRNA
ReactionCard
Thumb+Thumb+tRNA(Leu)Thumb+Thumb+L-leucyl-tRNA(Leu)
Thumb+Thumb+Thumb+tRNA(Leu)Thumb+Pyrophosphate+L-Leucyl-tRNA(Leu)
L-Leucine + Adenosine triphosphate + Hydrogen ion + tRNA(Leu) → Adenosine monophosphate + Pyrophosphate + L-Leucyl-tRNA(Leu)
ReactionCard
Thumb
a tRNAleu + L-leucine + ATP + H+ → an L-leucyl-[tRNAleu] + AMP + diphosphate
ReactionCard
Complex Reactions:
Thumb+Thumb+tRNA(Leu)Thumb+L-Leucyl-tRNA(Leu)+Thumb
Thumb+Thumb+tRNA(Leu)Thumb+Thumb+L-leucyl-tRNA(Leu)
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000014Adenosine monophosphateMetaboCard
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000170L-LeucineMetaboCard
PAMDB000633PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
aminoacyl-tRNA ligase activity
ATP binding
binding
catalytic activity
leucine-tRNA ligase activity
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
ligase activity, forming carbon-oxygen bonds
nucleoside binding
nucleotide binding
purine nucleoside binding
Process
biosynthetic process
cellular macromolecule biosynthetic process
cellular macromolecule metabolic process
leucyl-tRNA aminoacylation
macromolecule biosynthetic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
translation
tRNA aminoacylation
tRNA aminoacylation for protein translation
tRNA metabolic process
Gene Properties
Locus tag: PA3987
Strand: +
Entrez Gene ID: 878925
Accession: NP_252676.1
GI: 15599182
Sequence start: 4466925
Sequence End: 4469546
Sequence Length: 2621
Gene Sequence:
>PA3987
ATGCACGAGCAATATCAGCCACTCGAGATCGAAACCCAGGCCCAGAACTACTGGAAAGAACACCAGTCCTTCCTCGTCAGGGAGCTACCCGACAAGGAGAAGTTCTACTGCCTGTCGATGTTCCCGTATCCGAGCGGCAAGCTGCACATGGGCCACGTGCGCAACTACACCATCGGCGACGTGATCAGCCGCTACCACCGCATGCAGGGCCGCAACGTGCTGCAGCCGATGGGCTGGGACGCGTTCGGCATGCCGGCGGAAAACGCCGCGATGAAGAACAACGTCGCCCCGGCGGCCTGGACCTACGACAACATCGCCTACATGAAGTCGCAGCTGGACAGCCTCGGCCTGGCGATCGACTGGAGCCGCGAGGTCACCACCTGCAAGCCGGACTACTACCGCTGGGAGCAGTGGCTGTTCACCCGCCTGTTCGAAAAAGGGGTGATCTACCGCAAGAACGGCACGGTCAATTGGGATCCGGTCGACCAGACCGTACTCGCCAACGAGCAGGTGATCGACGGGCGCGGCTGGCGCTCCGGCGCGCTGATCGAGAAGCGCGAGATCCCGATGTACTACTTCAAGATCACCGCCTACGCCGAGGAACTGCTGGAGAGCCTCGACAACCTGCCCGGCTGGCCGGAGCAGGTGAAGACCATGCAGCGCAACTGGATCGGCAAGTCGCGCGGGATGGAGATCGGCTTCCCCTACGACCAGGCCTCCATCGGCCACGCCGGCCAACTGAAGGTGTTCACCACCCGTCCGGACACCCTGATGGGCGCCACCTACGTCGCCGTCGCCGCCGAACACCCGCTGGCCACCCAGGCAGCGCAGAACGACCCGCAGTTGCAGGCGTTCATCGACGAATGCAAGCGCGGCGGCGTCGCCGAGGCCGACATCGCCACCCAGGAGAAGAAAGGCATGGCCACTTCCCTGTTCGTCGAACACCCGCTGACCGGCGACAAGCTGCCGGTATGGGTCGCCAACTACGTGCTGATGAACTACGGCGAAGGCGCGGTGATGGCGGTGCCCGGCCATGACGAGCGCGACTTCGAGTTCGCCAACAAGTACGGCCTGCCGATCCGCCAGGTGATCGCCAAGGTCGAGGGCGACAACGACTTCGAGTCCAGCGTCTGGAAGGAGTGGTACGGCGCCAAGGACGAGTCCGTGCTCACCGTCAACTCCGGCAAGTACGACAACCTCGGCTACCAGGCCGCCTTCGACGCCATCGGCGCCGACCTCGAGGCCAAGGGCCTGGGCCAGGCGCGCACCCAGTTCCGCCTGCGCGACTGGGGCATCAGCCGCCAGCGCTACTGGGGCTGCCCGATCCCGATCATCCACTGCGAGGCTTGCGGCGACGTCCCGGTGCCGGCCGACCAGTTGCCGGTGGTGCTGCCGGAAGACGTGGTACCGGACGGCTCCGGTTCGCCGCTGGCGAAGATGCCGGAATTCTACGAATGCAACTGCCCGAAATGCGGCCAGCCGGCCAAGCGCGAAACCGACACCATGGACACCTTCGTCGAGTCGTCCTGGTATTTCGCCCGCTACGCCTGCCCGCAGTTCGAAGGCGGCATGCTGGACAGGAAGGCGGCCGACTACTGGCTGCCGGTCGACCAGTACATCGGCGGCATCGAGCACGCCATCCTGCACCTGCTCTATGCGCGCTTCTTCCACAAGCTGATGCGCGACGAAGGCCTGGTCGGCAGCGACGAACCGTTCAAGAACCTGCTGACCCAGGGCATGGTGGTCGCCGATACCTACTACCGGACCACCGCCAACGGCGGCAAGGACTGGTTCAACCCGGCCGACGTCGAGGTCGAGCGTGACGCCAAGGCCAAGGTCGTCGGCGCGCGCCTGAAGAGCGACGGCCAGCCGGTCGAGATCGGCGGCACCGAGAAGATGTCGAAGTCGAAGAACAACGGTGTCGACCCGCAATCGATGATCGACCAGTACGGTGCCGATACCTGCCGCCTGTTCATGATGTTCGCCTCGCCGCCGGACATGAGCCTGGAGTGGTCGGACGCCGGCGTCGAGGGCGCCAACCGCTTCCTCCGCCGCGTCTGGCGCCTGGCTCACGCCCACGTCAGCGCCGGCCTCCCCGGCGCGCTGGACGTCGCCGCCCTGAGCGACGCGCAGAAGCAGGTCCGCCGCGCCATCCACCTGGCGATCAGGCAGGCCAGCCAGGATGTCGGCCAGCACCACAAGTTCAACACCGCCATCGCCGCGGTGATGACCCTGATGAACGTGCTGGAGAAGGCGCCGAACCAGGACGCCCAGGACCGCGCCCTGATCCAGGAAGGCTTGGAAACCGTGGTCCTGCTGCTGGCGCCCATCACTCCGCACATCTGCCACGTGCTCTGGGGCCAGCTCGGCCATGCCGAAGCGGTGATCGATGCGCGCTGGCCGAGCGTCGACGAGAGCGCACTGGTGCAGGACACCCTGCAACTGGTGGTGCAGGTCAACGGCAAGCTGCGCGGACACATCGACGTGGCCGCCAGCGCCAGCCGCGAGGATGTCGAGGCCGCCGCGCGCGCCAACGAGAACGTGCTGCGTTTCACCGAAGGCCTGAGCATCCGCAAGGTCATCGTGGTACCGGGCAAACTGGTCAACATCGTCGCCAACTGA
Protein Properties
Protein Residues: 873
Protein Molecular Weight: 97.6 kDa
Protein Theoretical pI: 5.46
Hydropathicity (GRAVY score): -0.332
Charge at pH 7 (predicted): -19.02
Protein Sequence:
>PA3987
MHEQYQPLEIETQAQNYWKEHQSFLVRELPDKEKFYCLSMFPYPSGKLHMGHVRNYTIGDVISRYHRMQGRNVLQPMGWDAFGMPAENAAMKNNVAPAAWTYDNIAYMKSQLDSLGLAIDWSREVTTCKPDYYRWEQWLFTRLFEKGVIYRKNGTVNWDPVDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITAYAEELLESLDNLPGWPEQVKTMQRNWIGKSRGMEIGFPYDQASIGHAGQLKVFTTRPDTLMGATYVAVAAEHPLATQAAQNDPQLQAFIDECKRGGVAEADIATQEKKGMATSLFVEHPLTGDKLPVWVANYVLMNYGEGAVMAVPGHDERDFEFANKYGLPIRQVIAKVEGDNDFESSVWKEWYGAKDESVLTVNSGKYDNLGYQAAFDAIGADLEAKGLGQARTQFRLRDWGISRQRYWGCPIPIIHCEACGDVPVPADQLPVVLPEDVVPDGSGSPLAKMPEFYECNCPKCGQPAKRETDTMDTFVESSWYFARYACPQFEGGMLDRKAADYWLPVDQYIGGIEHAILHLLYARFFHKLMRDEGLVGSDEPFKNLLTQGMVVADTYYRTTANGGKDWFNPADVEVERDAKAKVVGARLKSDGQPVEIGGTEKMSKSKNNGVDPQSMIDQYGADTCRLFMMFASPPDMSLEWSDAGVEGANRFLRRVWRLAHAHVSAGLPGALDVAALSDAQKQVRRAIHLAIRQASQDVGQHHKFNTAIAAVMTLMNVLEKAPNQDAQDRALIQEGLETVVLLLAPITPHICHVLWGQLGHAEAVIDARWPSVDESALVQDTLQLVVQVNGKLRGHIDVAASASREDVEAAARANENVLRFTEGLSIRKVIVVPGKLVNIVAN
References
External Links:
Resource Link
Genome ID: PA3987
Entrez Gene ID: 878925
NCBI Protein ID: 15599182
General Reference: PaperBLAST - Find papers about PA3987 and its homologs