| Identification |
| Name: |
Queuine tRNA-ribosyltransferase |
| Synonyms: |
- Guanine insertion enzyme
- tRNA-guanine transglycosylase
|
| Gene Name: |
tgt |
| Enzyme Class: |
|
| Biological Properties |
| General Function: |
Involved in queuine tRNA-ribosyltransferase activity |
| Specific Function: |
Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) |
| Cellular Location: |
Not Available |
| KEGG Pathways: |
Not Available |
| KEGG Reactions: |
|
| SMPDB Reactions: |
|
 | + | tRNA guanine | → | 7-aminomethyl-7-deazaguanosine34 in tRNA | + |  |
| |
|
| PseudoCyc/BioCyc Reactions: |
|
| Complex Reactions: |
|
Guanine(34) in tRNA | + |  | → | queuosine(34) in tRNA | + | |
| | |
Guanine(34) in tRNA | + | | → | 7-aminomethyl-7-carbaguanine(34) in tRNA | + | |
| |
|
| Transports: |
Not Available |
| Metabolites: |
|
| GO Classification: |
| Function |
|---|
| catalytic activity | | queuine tRNA-ribosyltransferase activity | | transferase activity | | transferase activity, transferring glycosyl groups | | transferase activity, transferring pentosyl groups | | Process |
|---|
| cellular macromolecule metabolic process | | macromolecule metabolic process | | metabolic process | | ncRNA metabolic process | | queuosine biosynthetic process | | RNA metabolic process | | tRNA metabolic process | | tRNA modification | | tRNA processing |
|
| Gene Properties |
| Locus tag: |
PA3823 |
| Strand: |
- |
| Entrez Gene ID: |
879886 |
| Accession: |
NP_252512.1 |
| GI: |
15599018 |
| Sequence start: |
4279389 |
| Sequence End: |
4280507 |
| Sequence Length: |
1118 |
| Gene Sequence: |
>PA3823
ATGAACTTCGAGCTGCTGGCGACCGACGGCAAGGCCCGTCGCGGCCGCCTGACCTTCCCCCGCGGCGTGGTCGAGACCCCGGCCTTCATGCCGGTGGGCACCTACGGCACGGTGAAGGGCATGTTGCCGCGCGACATCGAGGACATCGGCGCGCAGATCATCCTCGGCAACACCTTCCATCTCTGGTTGCGCCCGGGCACCGAGGTGATCCAGCGCCACGGCGACCTGCACGACTTCATGCAGTGGAAAGGCCCGATCCTCACCGATTCCGGCGGTTTCCAGGTGTTCAGCCTGGGAGCCATGCGCAAGATCAAGGAGGAGGGCGTGACCTTCGCCTCGCCGGTGGACGGCGCCAAGGTCTTCATGGGGCCGGAGGAGTCCATGGCGGTGCAACGCGCCCTGGGCTCGGACATCGTGATGATCTTCGACGAATGCACGCCGTACCCGGCCGACCATGACGTCGCCAAGCGCTCCATGGAGCTGTCGCTGCGCTGGGCCAAGCGCTCGAAGATCGCCCATGGCGATAGCCCGTCGGCGCTGTTCGGCATCGTCCAGGGCGGTATGCACGAGGACCTGCGCCTGCGCTCGCTGGATGGCTTGCAGGAGATCGGCTTCGACGGCCTGGCCATCGGTGGTTTGTCGGTGGGCGAGCCGAAGGAAGAGATGATCCGCGTGCTCGATTTCCTGCCGCCGCAGATGCCGGCCGACAAGCCGCGCTACCTGATGGGCGTGGGCAAGCCGGAAGACCTGGTGGAAGGCGTGCGCCGCGGCGTGGACATGTTCGACTGCGTGATGCCGACGCGCAATGCGCGCAACGGCCACCTGTTCGTCGACAGCGGGGTGATCAAGATCCGCAACTCGGTACACAAGCACGACGACTCCACGCTCGACCCGACCTGCGACTGTTACACCTGCAAACATTTTTCCCGCGCCTATCTGCACCACTTGGACAAGTGCGGGGAAATGCTCGGTAGCATGCTCAATACCATCCACAACTTGCGGCATTATCAGCGGGTTATGGCTGGTTTGCGCGAGGCAATCCAACAGGGTACATTGGCCGCCTTTGTCGATGCCTTCTATGCCAAGCGCGGCCTTCCCACGCCGCCGCTGGATGCCTGA |
| Protein Properties |
| Protein Residues: |
372 |
| Protein Molecular Weight: |
41.2 kDa |
| Protein Theoretical pI: |
6.88 |
| Hydropathicity (GRAVY score): |
-0.215 |
| Charge at pH 7 (predicted): |
-0.81 |
| Protein Sequence: |
>PA3823
MNFELLATDGKARRGRLTFPRGVVETPAFMPVGTYGTVKGMLPRDIEDIGAQIILGNTFHLWLRPGTEVIQRHGDLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASPVDGAKVFMGPEESMAVQRALGSDIVMIFDECTPYPADHDVAKRSMELSLRWAKRSKIAHGDSPSALFGIVQGGMHEDLRLRSLDGLQEIGFDGLAIGGLSVGEPKEEMIRVLDFLPPQMPADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFVDSGVIKIRNSVHKHDDSTLDPTCDCYTCKHFSRAYLHHLDKCGEMLGSMLNTIHNLRHYQRVMAGLREAIQQGTLAAFVDAFYAKRGLPTPPLDA |
| References |
| External Links: |
|
| General Reference: |
PaperBLAST - Find papers about PA3823 and its homologs
|
