Bifunctional polymyxin resistance protein ArnA (PA3554)

Identification
Name: Bifunctional polymyxin resistance protein ArnA
Synonyms:
  • Polymyxin resistance protein PmrI
  • UDP-4-amino-4-deoxy-L-arabinose formyltransferase
  • ArnAFT
  • UDP-L-Ara4N formyltransferase
  • UDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acid decarboxylating
  • ArnADH
  • UDP-GlcUA decarboxylase
  • UDP-glucuronic acid dehydrogenase
Gene Name: arnA
Enzyme Class:
Biological Properties
General Function: Involved in hydroxymethyl-, formyl- and related transferase activity
Specific Function: Bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto- arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4- amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido- arabinose (UDP-L-Ara4FN). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides
Cellular Location: Not Available
KEGG Pathways:
  • Amino sugar and nucleotide sugar metabolism pae00520
KEGG Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
NAD + Uridine diphosphate glucuronic acid → Carbon dioxide + NADH + UDP-4-Keto-pyranose
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
N10-Formyl-THF + Uridine 5''-diphospho-{beta}-4-deoxy-4-amino-L-arabinose ↔ Hydrogen ion + Tetrahydrofolic acid + Uridine 5''-diphospho-{beta}-4-deoxy-4-formamido-L-arabinose
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
Uridine diphosphate glucuronic acid + NAD ↔ UDP-4-Keto-pyranose + Carbon dioxide + NADH + Hydrogen ion
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
N10-Formyl-THF + Uridine 5''-diphospho-{beta}-4-deoxy-4-amino-L-arabinose ↔ Tetrahydrofolic acid + Uridine 5''-diphospho-{beta}-4-deoxy-4-formamido-L-arabinose
ReactionCard
SMPDB Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
UDP-Glucuronic acid + NAD → Carbon dioxide + NADH + UDP-β-L-threo-pentapyranos-4-ulose
ReactionCard
Thumb+an N10-formyl-tetrahydrofolate+Thumb → Thumb+Thumb+a tetrahydrofolate+Thumb
Uridine 5''-diphospho-{beta}-4-deoxy-4-amino-L-arabinose + an N10-formyl-tetrahydrofolate + N10-Formyl-THF → UDP-4-Deoxy-4-formamido-beta-L-arabinose + Hydrogen ion + a tetrahydrofolate + Tetrahydrofolic acid
ReactionCard
Thumb+Thumb → Thumb+Thumb
N10-Formyl-THF + 4-Amino-4-deoxy-L-arabinose → Tetrahydrofolic acid + UDP-4-Deoxy-4-formamido-beta-L-arabinose
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
NAD + Uridine diphosphate glucuronic acid → Carbon dioxide + NADH + UDP-4-Keto-pyranose
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
N10-Formyl-THF + Uridine 5''-diphospho-{beta}-4-deoxy-4-amino-L-arabinose ↔ Hydrogen ion + Tetrahydrofolic acid + Uridine 5''-diphospho-{beta}-4-deoxy-4-formamido-L-arabinose
ReactionCard
Complex Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
UDP-Glucuronic acid + NAD → UDP-beta-L-Threo-pentapyranos-4-ulose + Carbon dioxide + NADH
ReactionCard
Thumb+Thumb → Thumb+Thumb
N10-Formyl-THF + 4-Amino-4-deoxy-L-arabinose → Tetrahydrofolic acid + UDP-4-Deoxy-4-formamido-beta-L-arabinose
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0018924-Amino-4-deoxy-L-arabinoseMetaboCard
PAMDB000568Carbon dioxideMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000213N10-Formyl-THFMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000424Tetrahydrofolic acidMetaboCard
PAMDB003589UDP-4-Deoxy-4-formamido-beta-L-arabinoseMetaboCard
PAMDB001697UDP-4-Keto-pyranoseMetaboCard
PAMDB003592UDP-beta-L-Threo-pentapyranos-4-uloseMetaboCard
PAMDB000364UDP-Glucuronic acidMetaboCard
PAMDB006357UDP-β-L-threo-pentapyranos-4-uloseMetaboCard
PAMDB001762Uridine 5''-diphospho-{beta}-4-deoxy-4-amino-L-arabinoseMetaboCard
PAMDB001763Uridine 5''-diphospho-{beta}-4-deoxy-4-formamido-L-arabinoseMetaboCard
PAMDB000646Uridine diphosphate glucuronic acidMetaboCard
GO Classification:
Function
binding
catalytic activity
coenzyme binding
cofactor binding
glycine hydroxymethyltransferase activity
hydroxymethyl-, formyl- and related transferase activity
methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
biosynthetic process
cellular metabolic process
metabolic process
Gene Properties
Locus tag: PA3554
Strand: +
Entrez Gene ID: 878473
Accession: NP_252244.1
GI: 15598750
Sequence start: 3982021
Sequence End: 3984009
Sequence Length: 1988
Gene Sequence: 
>PA3554
ATGACCTCGAAAGCCGTCGTCTTCGCCTACCACGACATCGGTTGCACCGGTATCGAAGCCCTGCTCAATGCCGGCTACGAGATCGCCGCCGTCTTCACCCATGCCGACGACCCACGGGAAAACACCTTCTACGCCTCGGTCGCACGCCTCTGCGCCGAGCGCGGCATTCCGCTGCACGCGCCCGAGGACGTGAACCATCCGCTGTGGCTGGAGCGTATCCGCCAACTGCGCCCGGACTTCCTGTTCTCCTTCTACTACCGCCGCCTGCTCGGCGCCGAGCTGCTCGCCTGCGCCGCACGCGGCGCCTACAACCTGCACGGTTCGCTGCTGCCGCGCTACCGCGGACGCGCCCCGGCGAACTGGGTGCTGGTCAACGGCGAAACGCAGACCGGGGTGACCCTGCATCGCATGATCGAGCGCGCCGACGCCGGGCCGATCCTCGCCCAGCAGGCCGTCGCCATCGACCCCGAGGACACCGCCCTGAGCCTGCACGGCAAGCTGCGCAAGGCCGCCGGCGCCCTGCTGCGCGACAGCCTGCCGCTGCTCGCCCTCGGCGTGCTGCCGGAAGTCGAGCAGGACGAGAGCCAGGCCAGCCACTTCGGCCGGCGCACCCCGGCGGACGGCCTGCTCGACTGGCACAGGCCGGCACGGCAGTTGTACGACCTGGTGCGCGCGGTGACCCAGCCCTACCCTGGCGCCTTCTGCCAGGTCGGCGAACAGAAGCTGATCGTCTGGAGCGCCGAGGTGGTCGCCGGCAACCACGGCCGCGAGCCGGGCAGCGTACTGTCCTGCGACCCGCTGCGGATCGCCTGCGGCGAGGACTCGCTGGTGCTGCGCTTCGGCCAGCGCGGCGAGCGCGGCCTGTACCTGGCCGGCACGCAACTGGCCACCGAGCTGGGCCTGGTCGAGGGCGCGCGCCTGCGTGGCGCGGCATGCAGTCCGCAGCGCCGCACGCGGGTGCTGATCCTCGGGGTCAACGGCTTCATCGGCAACCACCTGTCCGAACGCCTGCTGCGCGACGGTCGCTACGAGGTCCACGGCATGGACATCGGCTCCGACGCCATCGAACGGCTCAAGGCCGACCCGCATTTCCACTTCGTCGAAGGCGACATCGGCATCCATTCGGAGTGGCTCGAATACCATGTGAAGAAATGCGACGTGATCCTGCCGCTGGTGGCCATCGCCACGCCCATCGAGTACACGCGCAACCCGCTGCGGGTGTTCGAACTGGACTTCGAGGAAAACCTGCGGATCGTCCGCTACTGCGTGAAATACGGCAAACGCGTGGTGTTCCCTTCCACCTCCGAGGTCTACGGCATGTGCCAGGACCCGGACTTCGACGAAGACCGCTCGAACCTGGTGGTCGGGCCGATCAACAAGCAGCGCTGGATCTACTCGGTGTCCAAGCAGTTGCTCGACCGGGTGATCTGGGCCTACGGCCAGCAGGGCCTGCGCTTCACCCTGTTCCGTCCGTTCAACTGGATGGGCCCGCGCCTGGACCGCCTGGATTCGGCGCGGATCGGCAGCTCGCGGGCGATCACCCAGCTCATCCTGCACCTGGTCGAAGGCACGCCGATCCGCCTGGTCGACGGCGGCGCGCAGAAGCGCTGCTTCACCGACGTCGACGACGGCATCGAGGCCCTCGCGCGGATCATCGACAACCGCGACGGCCGCTGCGACGGGCAGATCGTCAACATCGGCAACCCGGACAACGAGGCGAGCATCCGCCAGCTCGGCGAGGAACTGCTGCGCCAGTTCGAGGCCCACCCGCTGCGCGCGCAGTTCCCGCCCTTCGCCGGCTTCCGCGAGGTGGAGAGCCGCAGCTTCTACGGCGACGGCTACCAGGACGTGGCCCACCGCAAGCCGAGCATCGACAACGCCCGGCGCCTGCTCGACTGGCAGCCCACCATCGAACTGCGCGAGACCATCGGCAAGACCCTCGACTTCTTCCTCCACGAAGCGCTCCGCGAGCGCGAGGCACAGGCGTGA
Protein Properties
Protein Residues: 662
Protein Molecular Weight: 74.4 kDa
Protein Theoretical pI: 6.68
Hydropathicity (GRAVY score): -0.253
Charge at pH 7 (predicted): -3.52
Protein Sequence: 
>PA3554
MTSKAVVFAYHDIGCTGIEALLNAGYEIAAVFTHADDPRENTFYASVARLCAERGIPLHAPEDVNHPLWLERIRQLRPDFLFSFYYRRLLGAELLACAARGAYNLHGSLLPRYRGRAPANWVLVNGETQTGVTLHRMIERADAGPILAQQAVAIDPEDTALSLHGKLRKAAGALLRDSLPLLALGVLPEVEQDESQASHFGRRTPADGLLDWHRPARQLYDLVRAVTQPYPGAFCQVGEQKLIVWSAEVVAGNHGREPGSVLSCDPLRIACGEDSLVLRFGQRGERGLYLAGTQLATELGLVEGARLRGAACSPQRRTRVLILGVNGFIGNHLSERLLRDGRYEVHGMDIGSDAIERLKADPHFHFVEGDIGIHSEWLEYHVKKCDVILPLVAIATPIEYTRNPLRVFELDFEENLRIVRYCVKYGKRVVFPSTSEVYGMCQDPDFDEDRSNLVVGPINKQRWIYSVSKQLLDRVIWAYGQQGLRFTLFRPFNWMGPRLDRLDSARIGSSRAITQLILHLVEGTPIRLVDGGAQKRCFTDVDDGIEALARIIDNRDGRCDGQIVNIGNPDNEASIRQLGEELLRQFEAHPLRAQFPPFAGFREVESRSFYGDGYQDVAHRKPSIDNARRLLDWQPTIELRETIGKTLDFFLHEALREREAQA
References
External Links:
Resource Link
Genome ID: PA3554
Entrez Gene ID: 878473
NCBI Protein ID: 15598750
General Reference: PaperBLAST - Find papers about PA3554 and its homologs