Identification
Name: 3-oxoacyl-[acyl-carrier-protein] synthase 2
Synonyms:
  • 3-oxoacyl-[acyl-carrier-protein] synthase II
  • Beta-ketoacyl-ACP synthase II
  • KAS II
Gene Name: fabF
Enzyme Class:
Biological Properties
General Function: Involved in transferase activity, transferring acyl groups other than amino-acyl groups
Specific Function: Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Has a preference for short chain acid substrates and may function to supply the octanoic substrates for lipoic acid biosynthesis
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Acetyl-[acyl-carrier protein]+Malonyl-[acyl-carrier protein]Acetoacetyl-[acp]+Thumb+Acyl-carrier protein
Acetyl-[acyl-carrier protein] + Malonyl-[acyl-carrier protein] ↔ Acetoacetyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Dodecanoyl-[acyl-carrier protein]+Malonyl-[acyl-carrier protein]3-Oxotetradecanoyl-[acp]+Thumb+Acyl-carrier protein
Dodecanoyl-[acyl-carrier protein] + Malonyl-[acyl-carrier protein] ↔ 3-Oxotetradecanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Butyryl-[acp]+Malonyl-[acyl-carrier protein]3-Oxohexanoyl-[acp]+Thumb+Acyl-carrier protein
Butyryl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxohexanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Hexanoyl-[acp]+Malonyl-[acyl-carrier protein]3-Oxooctanoyl-[acp]+Thumb+Acyl-carrier protein
Hexanoyl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxooctanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Octanoyl-[acp]+Malonyl-[acyl-carrier protein]3-Oxodecanoyl-[acp]+Thumb+Acyl-carrier protein
Octanoyl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxodecanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Decanoyl-[acp]+Malonyl-[acyl-carrier protein]3-Oxododecanoyl-[acp]+Thumb+Acyl-carrier protein
Decanoyl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxododecanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Tetradecanoyl-[acp]+Malonyl-[acyl-carrier protein]3-Oxohexadecanoyl-[acp]+Thumb+Acyl-carrier protein
Tetradecanoyl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxohexadecanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Hexadecanoyl-[acp]+Malonyl-[acyl-carrier protein]3-Oxostearoyl-[acp]+Acyl-carrier protein+Thumb
Hexadecanoyl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxostearoyl-[acp] + Acyl-carrier protein + Carbon dioxide
ReactionCard
SMPDB Reactions:
a malonyl-[acp]+a malonyl-[acp] methyl esterThumb+[ACP]+3-Ketoglutaryl-[acp] methyl ester
a malonyl-[acp] + a malonyl-[acp] methyl ester → Carbon dioxide + [ACP] + 3-Ketoglutaryl-[acp] methyl ester
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a malonyl-[acp]+Glutaryl-[acp] methyl ester a holo-[acyl-carrier protein]+Thumb+3-Ketopimeloyl-[acp] methyl ester
a malonyl-[acp] + Glutaryl-[acp] methyl ester → a holo-[acyl-carrier protein] + Carbon dioxide + 3-Ketopimeloyl-[acp] methyl ester
ReactionCard
2,3,4-saturated fatty acyl-[acp] +a malonyl-[acp]+ThumbThumb+a holo-[acyl-carrier protein]+3-oxoacyl-[acp]?
2,3,4-saturated fatty acyl-[acp] + a malonyl-[acp] + Hydrogen ionCarbon dioxide + a holo-[acyl-carrier protein] + 3-oxoacyl-[acp]?
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a malonyl-[acp]+Thumb+an acetyl-[acp]Thumb+a holo-[acyl-carrier protein]+acetoacetyl-[acp]
a malonyl-[acp] + Hydrogen ion + an acetyl-[acp] → Carbon dioxide + a holo-[acyl-carrier protein] + acetoacetyl-[acp]
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a palmitoleoyl-[acp]?+a malonyl-[acp]+Thumba holo-[acyl-carrier protein]+Thumb+3-oxo-cis-vaccenoyl-[acp]?
a palmitoleoyl-[acp]? + a malonyl-[acp] + Hydrogen ion → a holo-[acyl-carrier protein] + Carbon dioxide + 3-oxo-cis-vaccenoyl-[acp]?
ReactionCard
PseudoCyc/BioCyc Reactions:
Butyryl-ACP (n-C4:0ACP)+Thumb+Malonyl-[acyl-carrier protein]3 -Oxohexanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Butyryl-ACP (n-C4:0ACP) + Hydrogen ion + Malonyl-[acyl-carrier protein] → 3 -Oxohexanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Decanoyl-ACP (n-C10:0ACP)+Thumb+Malonyl-[acyl-carrier protein]3 -Oxododecanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Decanoyl-ACP (n-C10:0ACP) + Hydrogen ion + Malonyl-[acyl-carrier protein] → 3 -Oxododecanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Thumb+Malonyl-[acyl-carrier protein]+Palmitoyl-ACP (n-C16:0ACP)3 -Oxooctadecanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Hydrogen ion + Malonyl-[acyl-carrier protein] + Palmitoyl-ACP (n-C16:0ACP) → 3 -Oxooctadecanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Acetyl-ACP+Thumb+Malonyl-[acyl-carrier protein]acyl carrier protein+Acetoacetyl-ACP+Thumb
Acetyl-ACP + Hydrogen ion + Malonyl-[acyl-carrier protein] → acyl carrier protein + Acetoacetyl-ACP + Carbon dioxide
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Dodecanoyl-ACP (n-C12:0ACP)+Thumb+Malonyl-[acyl-carrier protein]3 -Oxotetradecanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Dodecanoyl-ACP (n-C12:0ACP) + Hydrogen ion + Malonyl-[acyl-carrier protein] → 3 -Oxotetradecanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Thumb+Hexanoyl-ACP (n-C6:0ACP)+Malonyl-[acyl-carrier protein]3 -Oxooctanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Hydrogen ion + Hexanoyl-ACP (n-C6:0ACP) + Malonyl-[acyl-carrier protein] → 3 -Oxooctanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Thumb+Malonyl-[acyl-carrier protein]+Myristoyl-ACP (n-C14:0ACP)3 -Oxohexadecanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Hydrogen ion + Malonyl-[acyl-carrier protein] + Myristoyl-ACP (n-C14:0ACP) → 3 -Oxohexadecanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Thumb+Malonyl-[acyl-carrier protein]+Octanoyl-ACP (n-C8:0ACP)3 -Oxodecanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Hydrogen ion + Malonyl-[acyl-carrier protein] + Octanoyl-ACP (n-C8:0ACP) → 3 -Oxodecanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Thumb+cis-hexadec-9-enoyl-[acyl-carrier protein] (n-C16:1)+Malonyl-[acyl-carrier protein]3 -oxo-cis-vacc-11-enoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Hydrogen ion + cis-hexadec-9-enoyl-[acyl-carrier protein] (n-C16:1) + Malonyl-[acyl-carrier protein] → 3 -oxo-cis-vacc-11-enoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Butyryl-[acp]+Malonyl-[acyl-carrier protein]3-Oxohexanoyl-[acp]+Thumb+Acyl-carrier protein
Butyryl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxohexanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Hexanoyl-[acp]+Malonyl-[acyl-carrier protein]3-Oxooctanoyl-[acp]+Thumb+Acyl-carrier protein
Hexanoyl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxooctanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Octanoyl-[acp]+Malonyl-[acyl-carrier protein]3-Oxodecanoyl-[acp]+Thumb+Acyl-carrier protein
Octanoyl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxodecanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Decanoyl-[acp]+Malonyl-[acyl-carrier protein]3-Oxododecanoyl-[acp]+Thumb+Acyl-carrier protein
Decanoyl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxododecanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Tetradecanoyl-[acp]+Malonyl-[acyl-carrier protein]3-Oxohexadecanoyl-[acp]+Thumb+Acyl-carrier protein
Tetradecanoyl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxohexadecanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Acetyl-[acyl-carrier protein]+Malonyl-[acyl-carrier protein]Acetoacetyl-[acp]+Thumb+Acyl-carrier protein
Acetyl-[acyl-carrier protein] + Malonyl-[acyl-carrier protein] ↔ Acetoacetyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Dodecanoyl-[acyl-carrier protein]+Malonyl-[acyl-carrier protein]3-Oxotetradecanoyl-[acp]+Thumb+Acyl-carrier protein
Dodecanoyl-[acyl-carrier protein] + Malonyl-[acyl-carrier protein] ↔ 3-Oxotetradecanoyl-[acp] + Carbon dioxide + Acyl-carrier protein
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Hexadecanoyl-[acp]+Malonyl-[acyl-carrier protein]3-Oxostearoyl-[acp]+Acyl-carrier protein+Thumb
Hexadecanoyl-[acp] + Malonyl-[acyl-carrier protein] ↔ 3-Oxostearoyl-[acp] + Acyl-carrier protein + Carbon dioxide
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(Z)-hexadec-11-enoyl-[acyl-carrier-protein]+malonyl-[acyl-carrier-protein](Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein]+Thumb+[acyl-carrier-protein]
(Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] → (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + Carbon dioxide + [acyl-carrier-protein]
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a malonyl-[acp]+a malonyl-[acp] methyl esterThumb+[ACP]+3-Ketoglutaryl-[acp] methyl ester
a malonyl-[acp] + a malonyl-[acp] methyl ester → Carbon dioxide + [ACP] + 3-Ketoglutaryl-[acp] methyl ester
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a malonyl-[acp]+Glutaryl-[acp] methyl ester a holo-[acyl-carrier protein]+Thumb+3-Ketopimeloyl-[acp] methyl ester
a malonyl-[acp] + Glutaryl-[acp] methyl ester → a holo-[acyl-carrier protein] + Carbon dioxide + 3-Ketopimeloyl-[acp] methyl ester
ReactionCard
2,3,4-saturated fatty acyl-[acp] +a malonyl-[acp]+ThumbThumb+a holo-[acyl-carrier protein]+3-oxoacyl-[acp]?
2,3,4-saturated fatty acyl-[acp] + a malonyl-[acp] + Hydrogen ionCarbon dioxide + a holo-[acyl-carrier protein] + 3-oxoacyl-[acp]?
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a malonyl-[acp]+Thumb+an acetyl-[acp]Thumb+a holo-[acyl-carrier protein]+acetoacetyl-[acp]
a malonyl-[acp] + Hydrogen ion + an acetyl-[acp] → Carbon dioxide + a holo-[acyl-carrier protein] + acetoacetyl-[acp]
ReactionCard
a palmitoleoyl-[acp]?+a malonyl-[acp]+Thumba holo-[acyl-carrier protein]+Thumb+3-oxo-cis-vaccenoyl-[acp]?
a palmitoleoyl-[acp]? + a malonyl-[acp] + Hydrogen ion → a holo-[acyl-carrier protein] + Carbon dioxide + 3-oxo-cis-vaccenoyl-[acp]?
ReactionCard
Thumb
an acetyl-[acp] + a malonyl-[acp] + H+ → a holo-[acyl-carrier protein] + an acetoacetyl-[acp] + CO2
ReactionCard
PAMDB001633PAMDB000568
Complex Reactions:
Butyryl-ACP (n-C4:0ACP)+Thumb+Malonyl-[acyl-carrier protein]3 -Oxohexanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Butyryl-ACP (n-C4:0ACP) + Hydrogen ion + Malonyl-[acyl-carrier protein] → 3 -Oxohexanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
ReactionCard
Decanoyl-ACP (n-C10:0ACP)+Thumb+Malonyl-[acyl-carrier protein]3 -Oxododecanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Decanoyl-ACP (n-C10:0ACP) + Hydrogen ion + Malonyl-[acyl-carrier protein] → 3 -Oxododecanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
ReactionCard
Thumb+Malonyl-[acyl-carrier protein]+Palmitoyl-ACP (n-C16:0ACP)3 -Oxooctadecanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Hydrogen ion + Malonyl-[acyl-carrier protein] + Palmitoyl-ACP (n-C16:0ACP) → 3 -Oxooctadecanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
ReactionCard
Acetyl-ACP+Thumb+Malonyl-[acyl-carrier protein]acyl carrier protein+Acetoacetyl-ACP+Thumb
Acetyl-ACP + Hydrogen ion + Malonyl-[acyl-carrier protein] → acyl carrier protein + Acetoacetyl-ACP + Carbon dioxide
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Dodecanoyl-ACP (n-C12:0ACP)+Thumb+Malonyl-[acyl-carrier protein]3 -Oxotetradecanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Dodecanoyl-ACP (n-C12:0ACP) + Hydrogen ion + Malonyl-[acyl-carrier protein] → 3 -Oxotetradecanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Thumb+Hexanoyl-ACP (n-C6:0ACP)+Malonyl-[acyl-carrier protein]3 -Oxooctanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Hydrogen ion + Hexanoyl-ACP (n-C6:0ACP) + Malonyl-[acyl-carrier protein] → 3 -Oxooctanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Thumb+Malonyl-[acyl-carrier protein]+Myristoyl-ACP (n-C14:0ACP)3 -Oxohexadecanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Hydrogen ion + Malonyl-[acyl-carrier protein] + Myristoyl-ACP (n-C14:0ACP) → 3 -Oxohexadecanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Thumb+Malonyl-[acyl-carrier protein]+Octanoyl-ACP (n-C8:0ACP)3 -Oxodecanoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Hydrogen ion + Malonyl-[acyl-carrier protein] + Octanoyl-ACP (n-C8:0ACP) → 3 -Oxodecanoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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Thumb+cis-hexadec-9-enoyl-[acyl-carrier protein] (n-C16:1)+Malonyl-[acyl-carrier protein]3 -oxo-cis-vacc-11-enoyl-[acyl-carrier protein]+acyl carrier protein+Thumb
Hydrogen ion + cis-hexadec-9-enoyl-[acyl-carrier protein] (n-C16:1) + Malonyl-[acyl-carrier protein] → 3 -oxo-cis-vacc-11-enoyl-[acyl-carrier protein] + acyl carrier protein + Carbon dioxide
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(Z)-hexadec-11-enoyl-[acyl-carrier-protein]+malonyl-[acyl-carrier-protein](Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein]+Thumb+[acyl-carrier-protein]
(Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] → (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + Carbon dioxide + [acyl-carrier-protein]
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000568Carbon dioxideMetaboCard
PAMDB001933Heptadecenoic acidMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB003556malonyl-CoA methyl esterMetaboCard
PAMDB001901Margaric acidMetaboCard
GO Classification:
Function
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
biosynthetic process
carboxylic acid metabolic process
cellular metabolic process
fatty acid biosynthetic process
fatty acid metabolic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxoacid metabolic process
Gene Properties
Locus tag: PA2965
Strand: -
Entrez Gene ID: 879894
Accession: NP_251655.1
GI: 15598161
Sequence start: 3323574
Sequence End: 3324818
Sequence Length: 1244
Gene Sequence:
>PA2965
GTGTCGCGTAGACGCGTCGTCATTACTGGCATGGGCATGCTGTCGCCGCTGGGCCTGGATGTCCCGAGCAGTTGGGAAGGTATTCTCGCCGGTCGCAGCGGGATCGCCCCGATCGAGCATATGGACCTGTCCGCCTATTCCACCCGCTTCGGTGGTTCGGTCAAGGGTTTCAATGTCGAGGAATACCTGTCCGCCAAGGAAGCCCGCAAGCTCGACCTGTTCATCCAGTACGGTCTCGCCGCCAGCTTCCAGGCCGTTCGCGATTCCGGCCTGGAGGTCACCGACGCCAATCGTGAGCGTATCGGCGTATCCATGGGTTCCGGTATCGGCGGCCTGACCAATATCGAGAACAACTGCCGATCCCTGTTCGAGCAGGGGCCGCGGCGCATCTCGCCGTTCTTCGTGCCCGGTTCGATCATCAACATGGTTTCCGGCTTCCTGTCGATCCACCTGGGTCTGCAGGGGCCCAACTACGCCCTCACCACCGCCTGCACCACCGGTACCCACAGCATTGGCATGGCTGCTCGCAACATCGCCTATGGCGAGGCCGACGTGATGGTCGCCGGCGGTTCCGAAATGGCTGCCTGCGGTCTCGGCCTGGGTGGTTTCGGCGCGGCTCGCGCGCTCTCCACTCGCAACGACGAGCCGACCCGTGCCAGCCGTCCGTGGGACCGGGATCGCGACGGCTTCGTCCTGTCCGACGGTTCCGGCGCCCTGGTGCTCGAAGAACTGGAGCACGCCCGTGCCCGCGGCGCGCGGATCTATGCCGAGCTGGTTGGCTTCGGCATGAGCGGCGACGCCTTCCACATGACCGCGCCGCCGGAAGACGGCGCCGGTGCCGCCCGCTGCATGAAGAACGCGCTGCGCGATGCCGGCCTGGATCCTCGTCAGGTCGATTACATCAACGCCCATGGCACCTCCACCCCGGCCGGCGACATCGCCGAGATCGCCGCGGTGAAGAGCGTGTTCGGCGAGCATGCCCATGCGCTGTCGATGAGTTCGACCAAGTCGATGACCGGCCACCTGTTAGGTGCCGCCGGTGCGGTGGAGGCGATCTTCAGCGTGCTGGCCCTGCGCGACCAGGTCGCCCCGCCGACCATCAACCTGGACAACCCGGACGAAGGTTGCGACCTCGACCTGGTGGCCCATGAAGCCAAGCCGCGCAAGATCGACGTGGCCCTGTCGAACTCGTTCGGTTTCGGCGGGACCAACGGCACCCTGGTGTTCCGCAGGTTCGCCGACTGA
Protein Properties
Protein Residues: 414
Protein Molecular Weight: 43.5 kDa
Protein Theoretical pI: 5.81
Hydropathicity (GRAVY score): -0.029
Charge at pH 7 (predicted): -6.74
Protein Sequence:
>PA2965
MSRRRVVITGMGMLSPLGLDVPSSWEGILAGRSGIAPIEHMDLSAYSTRFGGSVKGFNVEEYLSAKEARKLDLFIQYGLAASFQAVRDSGLEVTDANRERIGVSMGSGIGGLTNIENNCRSLFEQGPRRISPFFVPGSIINMVSGFLSIHLGLQGPNYALTTACTTGTHSIGMAARNIAYGEADVMVAGGSEMAACGLGLGGFGAARALSTRNDEPTRASRPWDRDRDGFVLSDGSGALVLEELEHARARGARIYAELVGFGMSGDAFHMTAPPEDGAGAARCMKNALRDAGLDPRQVDYINAHGTSTPAGDIAEIAAVKSVFGEHAHALSMSSTKSMTGHLLGAAGAVEAIFSVLALRDQVAPPTINLDNPDEGCDLDLVAHEAKPRKIDVALSNSFGFGGTNGTLVFRRFAD
References
External Links:
Resource Link
Genome ID: PA2965
Entrez Gene ID: 879894
NCBI Protein ID: 15598161
General Reference: PaperBLAST - Find papers about PA2965 and its homologs