Lipoyl synthase (PA2862)

Identification
Name: Lipoyl synthase
Synonyms:
  • Lip-syn
  • LS
  • Lipoate synthase
  • Lipoic acid synthase
  • Sulfur insertion protein lipA
Gene Name: lipA
Enzyme Class:
Biological Properties
General Function: Involved in catalytic activity
Specific Function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. Free octanoate is not a substrate for lipA
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Protein N6-(octanoyl)lysine+2 Sulfur donor+2 Thumb+Protein N6-(octanoyl)lysine ↔ Protein N6-(lipoyl)lysine+2 Thumb+2 Thumb+Protein N6-(lipoyl)lysine
Protein N6-(octanoyl)lysine + 2 Sulfur donor + 2 S-Adenosylmethionine + Protein N6-(octanoyl)lysine ↔ Protein N6-(lipoyl)lysine + 2 L-Methionine + 2 5'-Deoxyadenosine + Protein N6-(lipoyl)lysine
ReactionCard
Octanoyl-[acp]+2 Sulfur donor+2 Thumb ↔ Lipoyl-[acp]+2 Thumb+2 Thumb
Octanoyl-[acp] + 2 Sulfur donor + 2 S-Adenosylmethionine ↔ Lipoyl-[acp] + 2 L-Methionine + 2 5'-Deoxyadenosine
ReactionCard
SMPDB Reactions:
Octanoyl-[acyl-carrier protein] +2 a sulfur donor+2 S-adenosyl-L-methionine → Lipoyl-ACP+2 Thumb+Thumb
Octanoyl-[acyl-carrier protein] + 2 a sulfur donor + 2 S-adenosyl-L-methionine → Lipoyl-ACP + 2 L-Methionine + 5'-Deoxyadenosine
ReactionCard
Protein N6-(octanoyl)lysine+2 a sulfur donor+2 S-adenosyl-L-methionine → Protein N6-(lipoyl)lysine+2 Thumb+2 Thumb
Protein N6-(octanoyl)lysine + 2 a sulfur donor + 2 S-adenosyl-L-methionine → Protein N6-(lipoyl)lysine + 2 L-Methionine + 2 5'-Deoxyadenosine
ReactionCard
a [lipoyl-carrier protein] N6-octanoyl-L-lysine+2 S-adenosyl-L-methionine+2 a sulfurated [sulfur carrier]?+2 Reduced ferredoxin → Protein N6-(lipoyl)lysine+2 Thumb+2 Thumb+2 an unsulfurated [sulfur carrier]+2 Oxidized ferredoxin
a [lipoyl-carrier protein] N6-octanoyl-L-lysine + 2 S-adenosyl-L-methionine + 2 a sulfurated [sulfur carrier]? + 2 Reduced ferredoxin → Protein N6-(lipoyl)lysine + 2 5'-Deoxyadenosine + 2 L-Methionine + 2 an unsulfurated [sulfur carrier] + 2 Oxidized ferredoxin
ReactionCard
PseudoCyc/BioCyc Reactions:
[4Fe-4S] iron-sulfur cluster+2 Thumb+Thumb+Thumb+octanoate (protein bound) → [2Fe-2S] iron-sulfur cluster+2 Thumb+2 Thumb+lipoate (protein bound)+2 Thumb+Thumb
[4Fe-4S] iron-sulfur cluster + 2 S-Adenosylmethionine + Hydrogen ion + NAD + octanoate (protein bound) → [2Fe-2S] iron-sulfur cluster + 2 5'-Deoxyadenosine + 2 Iron + lipoate (protein bound) + 2 L-Methionine + NADH
ReactionCard
Protein N6-(octanoyl)lysine+2 Sulfur donor+2 Thumb+Protein N6-(octanoyl)lysine ↔ Protein N6-(lipoyl)lysine+2 Thumb+2 Thumb+Protein N6-(lipoyl)lysine
Protein N6-(octanoyl)lysine + 2 Sulfur donor + 2 S-Adenosylmethionine + Protein N6-(octanoyl)lysine ↔ Protein N6-(lipoyl)lysine + 2 L-Methionine + 2 5'-Deoxyadenosine + Protein N6-(lipoyl)lysine
ReactionCard
Octanoyl-[acp]+2 Sulfur donor+2 Thumb ↔ Lipoyl-[acp]+2 Thumb+2 Thumb
Octanoyl-[acp] + 2 Sulfur donor + 2 S-Adenosylmethionine ↔ Lipoyl-[acp] + 2 L-Methionine + 2 5'-Deoxyadenosine
ReactionCard
Octanoyl-[acyl-carrier protein] +2 a sulfur donor+2 S-adenosyl-L-methionine → Lipoyl-ACP+2 Thumb+Thumb
Octanoyl-[acyl-carrier protein] + 2 a sulfur donor + 2 S-adenosyl-L-methionine → Lipoyl-ACP + 2 L-Methionine + 5'-Deoxyadenosine
ReactionCard
Protein N6-(octanoyl)lysine+2 a sulfur donor+2 S-adenosyl-L-methionine → Protein N6-(lipoyl)lysine+2 Thumb+2 Thumb
Protein N6-(octanoyl)lysine + 2 a sulfur donor + 2 S-adenosyl-L-methionine → Protein N6-(lipoyl)lysine + 2 L-Methionine + 2 5'-Deoxyadenosine
ReactionCard
Complex Reactions:
[4Fe-4S] iron-sulfur cluster+2 Thumb+Thumb+Thumb+octanoate (protein bound) → [2Fe-2S] iron-sulfur cluster+2 Thumb+2 Thumb+lipoate (protein bound)+2 Thumb+Thumb
[4Fe-4S] iron-sulfur cluster + 2 S-Adenosylmethionine + Hydrogen ion + NAD + octanoate (protein bound) → [2Fe-2S] iron-sulfur cluster + 2 5'-Deoxyadenosine + 2 Iron + lipoate (protein bound) + 2 L-Methionine + NADH
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0015835'-DeoxyadenosineMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000486Hydrogen sulfideMetaboCard
PAMDB000172IronMetaboCard
PAMDB000175L-MethionineMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000289S-AdenosylmethionineMetaboCard
GO Classification:
Function
4 iron, 4 sulfur cluster binding
binding
catalytic activity
iron-sulfur cluster binding
lipoate synthase activity
metal cluster binding
sulfurtransferase activity
transferase activity
transferase activity, transferring sulfur-containing groups
Process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
lipoate biosynthetic process
lipoic acid biosynthetic process
lipoic acid metabolic process
metabolic process
Gene Properties
Locus tag: PA2862
Strand: +
Entrez Gene ID: 882662
Accession: NP_251552.1
GI: 15598058
Sequence start: 3214282
Sequence End: 3215217
Sequence Length: 935
Gene Sequence: 
>PA2862
ATGAAGAAGAAGTCTCTGCTCCCCCTCGGCCTGGCCATCGGTCTCGCCTCTCTCGCTGCCAGCCCTCTGATCCAGGCCAGCACCTACACCCAGACCAAATACCCCATCGTGCTGGCCCACGGCATGCTCGGCTTCGACAACATCCTCGGGGTCGACTACTGGTTCGGCATTCCCAGCGCCTTGCGCCGTGACGGTGCCCAGGTCTACGTCACCGAAGTCAGCCAGTTGGACACCTCGGAAGTCCGCGGCGAGCAGTTGCTGCAACAGGTGGAGGAAATCGTCGCCCTCAGCGGCCAGCCCAAGGTCAACCTGATCGGCCACAGCCACGGCGGGCCGACCATCCGCTACGTCGCCGCCGTACGTCCCGACCTGATCGCTTCCGCCACCAGCGTCGGCGCCCCGCACAAGGGTTCGGACACCGCCGACTTCCTGCGCCAGATCCCACCGGGTTCGGCCGGCGAGGCAGTCCTCTCCGGGCTGGTCAACAGCCTCGGCGCGCTGATCAGCTTCCTTTCCAGCGGCAGCACCGGTACGCAGAATTCACTGGGCTCGCTGGAGTCGCTGAACAGCGAGGGTGCCGCGCGCTTCAACGCCAAGTACCCGCAGGGCATCCCCACCTCGGCCTGCGGCGAAGGCGCCTACAAGGTCAACGGCGTGAGCTATTACTCCTGGAGCGGTTCCTCGCCGCTGACCAACTTCCTCGATCCGAGCGACGCCTTCCTCGGCGCCTCGTCGCTGACCTTCAAGAACGGCACCGCCAACGACGGCCTGGTCGGCACCTGCAGTTCGCACCTGGGCATGGTGATCCGCGACAACTACCGGATGAACCACCTGGACGAGGTGAACCAGGTCTTCGGCCTCACCAGCCTGTTCGAGACCAGCCCGGTCAGCGTCTACCGCCAGCACGCCAACCGCCTGAAGAACGCCAGCCTGTAG
Protein Properties
Protein Residues: 311
Protein Molecular Weight: 32.7 kDa
Protein Theoretical pI: 6.88
Hydropathicity (GRAVY score): -0.008
Charge at pH 7 (predicted): -0.39
Protein Sequence: 
>PA2862
MKKKSLLPLGLAIGLASLAASPLIQASTYTQTKYPIVLAHGMLGFDNILGVDYWFGIPSALRRDGAQVYVTEVSQLDTSEVRGEQLLQQVEEIVALSGQPKVNLIGHSHGGPTIRYVAAVRPDLIASATSVGAPHKGSDTADFLRQIPPGSAGEAVLSGLVNSLGALISFLSSGSTGTQNSLGSLESLNSEGAARFNAKYPQGIPTSACGEGAYKVNGVSYYSWSGSSPLTNFLDPSDAFLGASSLTFKNGTANDGLVGTCSSHLGMVIRDNYRMNHLDEVNQVFGLTSLFETSPVSVYRQHANRLKNASL
References
External Links:
Resource Link
Genome ID: PA2862
Entrez Gene ID: 882662
NCBI Protein ID: 15598058
General Reference: PaperBLAST - Find papers about PA2862 and its homologs