Siroheme synthase (PA2611)

Identification
Name: Siroheme synthase
Synonyms:
  • Uroporphyrinogen-III C-methyltransferase
  • Urogen III methylase
  • SUMT
  • Uroporphyrinogen III methylase
  • UROM
  • Precorrin-2 dehydrogenase
  • Sirohydrochlorin ferrochelatase
Gene Name: cysG
Enzyme Class:
Biological Properties
General Function: Involved in methyltransferase activity
Specific Function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD- dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
2 Thumb+Thumb → 2 Thumb+Thumb+Thumb
2 S-Adenosylmethionine + Uroporphyrinogen III → 2 S-Adenosylhomocysteine + Precorrin 2 + Hydrogen ion
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Thumb+Thumb → Thumb+Thumb+Thumb
Precorrin 2 + NAD → Hydrogen ion + NADH + Sirohydrochlorin
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Thumb+2 Thumb ↔ Fe2++Thumb
Siroheme + 2 Hydrogen ion ↔ Fe2+ + Sirohydrochlorin
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2 Thumb+Thumb ↔ 2 Thumb+Thumb
2 S-Adenosylmethionine + Uroporphyrinogen III ↔ 2 S-Adenosylhomocysteine + Precorrin 2
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Thumb+Thumb ↔ Thumb+Thumb+Thumb
Precorrin 2 + NAD ↔ Sirohydrochlorin + NADH + Hydrogen ion
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2 Thumb+Thumb+Thumb ↔ 2 Thumb+Thumb
2 S-Adenosylmethionine + Uroporphyrinogen III + Precorrin-1 ↔ 2 S-Adenosylhomocysteine + Precorrin 2
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SMPDB Reactions:
Thumb+S-adenosyl-L-methionine → Thumb+Thumb+Thumb
Uroporphyrinogen III + S-adenosyl-L-methionine → Hydrogen ion + S-Adenosylhomocysteine + Precorrin-1
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Thumb+S-adenosyl-L-methionine → Precorrin-2+Thumb
Precorrin-1 + S-adenosyl-L-methionine → Precorrin-2 + S-Adenosylhomocysteine
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Precorrin-2+Thumb → Thumb+Thumb+Thumb
Precorrin-2 + NAD → NADH + Hydrogen ion + Sirohydrochlorin
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Thumb+Thumb → 2 Thumb+Thumb
Sirohydrochlorin + Iron → 2 Hydrogen ion + Siroheme
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Thumb+Thumb ↔ Thumb+Thumb+2 Thumb
Precorrin 2 + NAD ↔ Sirohydrochlorin + NADH + 2 Hydrogen ion
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PseudoCyc/BioCyc Reactions:
Thumb+Thumb → 3 Thumb+Thumb
Iron + Sirohydrochlorin → 3 Hydrogen ion + Siroheme
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Thumb+Thumb → Thumb+Thumb+Thumb
Precorrin 2 + NAD → Hydrogen ion + NADH + Sirohydrochlorin
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Complex Reactions:
Thumb+2 Thumb → Thumb+Thumb
Siroheme + 2 Hydrogen ion → Sirohydrochlorin + Iron
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB005697Fe2+MetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000172IronMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB001029Precorrin 2MetaboCard
PAMDB001963Precorrin-1MetaboCard
PAMDB000205S-AdenosylhomocysteineMetaboCard
PAMDB000289S-AdenosylmethionineMetaboCard
PAMDB001040SirohemeMetaboCard
PAMDB001041SirohydrochlorinMetaboCard
PAMDB000201Uroporphyrin IIIMetaboCard
PAMDB000248Uroporphyrinogen IIIMetaboCard
GO Classification:
Function
binding
catalytic activity
methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
metabolic process
nitrogen compound metabolic process
oxidation reduction
porphyrin biosynthetic process
porphyrin metabolic process
tetrapyrrole metabolic process
Gene Properties
Locus tag: PA2611
Strand: -
Entrez Gene ID: 882317
Accession: NP_251301.1
GI: 15597807
Sequence start: 2952017
Sequence End: 2953414
Sequence Length: 1397
Gene Sequence: 
>PA2611
ATGGATTTCCTGCCGCTGTTCCACTCCTTGCAAGGGCGCCTCGCGCTGGTGGTCGGCGGCGGCGAAGTCGCCCTGCGCAAGGCGCGCCTGCTGGCCGACGCCGGCGCGCGCCTGCGCGTGGTCGCGCCGCAGATCCACATCGAACTGCGGCATCTGGTGGAGCAGGGCGGCGGCGAGCTGCTCGAACGCGACTACCAGGACGGCGACCAGCCCGGCTGCGCCCTGATCATCGCCGCCACCGACGACGAACCGCTGAACGCCGAGGTGTCGCGAGCGGCCAATGCGCGCGGGATACCGGTCAACGTGGTGGACGCGCCGGCCCTGTGCAGCGTGATCTTCCCGGCCATCGTCGACCGTTCGCCGCTGGTGGTGGCGGTCTCCAGCGGCGGCGATGCGCCCGTGCTGGCGCGGCTGATCCGGGCCAAGCTGGAAACCTGGATTCCCTCGACCTACGGGCAACTGGCCGGACTCGCCAGCCGCTTCCGCCACCGGGTCAAGGAATTGCTACCCGACCTGCAGCAGCGCCGGGTGTTCTGGGAAAACCTGTTCCAGGGCGAGATCGCCGAGCGTGTCCTGGCCGGCCGTCCGGCGGAAGCCGAGCGTCTCCTGGAGGAGCACCTGGCCGGCGGCCTCGCGCACATAGCCACGGGCGAGGTATATCTGGTCGGGGCCGGGCCGGGGGATCCGGACCTGCTGACGTTCCGTGCCCTGCGCCTGATGCAGCAGGCCGACGTGGTGCTCTACGATCGCCTGGTGGCGCCGTCGATCCTCGAATTATGCCGCCGCGATGCCGAACGCCTGTACGTCGGCAAGCGCCGCGCCGAACATGCGGTGCCGCAGGATCGGATCAACCGCCTGCTGGTGGAGCTGGCCAGCCAGGGCAAGCGCGTGCTGCGCCTGAAGGGTGGCGACCCGTTCATCTTCGGCCGCGGCGGTGAGGAAATCGACGAACTGGCTGCCCACGGCATACCTTTCCAGGTCGTGCCGGGAATCACCGCGGCCAGCGGCTGCGCGGCCTACGCCGGGATTCCACTGACTCATCGCGATCATGCGCAATCGGTTCGTTTCGTCACCGGTCATCTGAAGGACGGCACCACCGACCTGCCGTGGCAGGACCTGGTAGCGCCTGGCCAGACCCTGGTGTTCTACATGGGCCTGGTGGGATTGCCGGTGATCTGCGAGCAACTGGTCGCCCACGGTCGCTCCGCACAGACCCCGGCGGCGCTGATCCAGCAGGGGACCACCGCCCAGCAGCGGGTATTCACCGGAACCCTGGAAAACCTGCCGCAACTGGTGGCTGAGCACGAAGTGCATGCGCCGACCCTGGTGATCGTCGGCGAAGTCGTGCAACTGCGCGACAAGCTGGCCTGGTTCGAAGGCGCCCGCGAGGACGCCTGA
Protein Properties
Protein Residues: 465
Protein Molecular Weight: 50.4 kDa
Protein Theoretical pI: 6.28
Hydropathicity (GRAVY score): 0.053
Charge at pH 7 (predicted): -5.76
Protein Sequence: 
>PA2611
MDFLPLFHSLQGRLALVVGGGEVALRKARLLADAGARLRVVAPQIHIELRHLVEQGGGELLERDYQDGDQPGCALIIAATDDEPLNAEVSRAANARGIPVNVVDAPALCSVIFPAIVDRSPLVVAVSSGGDAPVLARLIRAKLETWIPSTYGQLAGLASRFRHRVKELLPDLQQRRVFWENLFQGEIAERVLAGRPAEAERLLEEHLAGGLAHIATGEVYLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPSILELCRRDAERLYVGKRRAEHAVPQDRINRLLVELASQGKRVLRLKGGDPFIFGRGGEEIDELAAHGIPFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTGHLKDGTTDLPWQDLVAPGQTLVFYMGLVGLPVICEQLVAHGRSAQTPAALIQQGTTAQQRVFTGTLENLPQLVAEHEVHAPTLVIVGEVVQLRDKLAWFEGAREDA
References
External Links:
Resource Link
Genome ID: PA2611
Entrez Gene ID: 882317
NCBI Protein ID: 15597807
General Reference: PaperBLAST - Find papers about PA2611 and its homologs