Bifunctional protein folD (PA1796)

Identification
Name: Bifunctional protein folD
Synonyms:
  • Methylenetetrahydrofolate dehydrogenase
  • Methenyltetrahydrofolate cyclohydrolase
Gene Name: folD
Enzyme Class:
Biological Properties
General Function: Involved in nucleotide binding
Specific Function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate. This enzyme is specific for NADP
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb
Water + 5,10-Methenyltetrahydrofolate ↔ N10-Formyl-THF + Hydrogen ion
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Thumb+Thumb ↔ Thumb+Thumb+Thumb
5,10-Methylene-THF + NADP ↔ 5,10-Methenyltetrahydrofolate + NADPH + Hydrogen ion
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Thumb+Thumb ↔ Thumb+Thumb
5,10-Methylene-THF + NADP ↔ 5,10-Methenyltetrahydrofolate + NADPH
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Thumb+Thumb ↔ Thumb+Thumb
5,10-Methenyltetrahydrofolate + Water ↔ N10-Formyl-THF + Hydrogen ion
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SMPDB Reactions:
10-Formyltetrahydrofolate+Thumb ↔ 5,10-Methenyltetrahydrofolic acid +Thumb
10-Formyltetrahydrofolate + Hydrogen ion ↔ 5,10-Methenyltetrahydrofolic acid + Water
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5,10-Methenyltetrahydrofolic acid +NADPH+Thumb → 5,10-Methylene-THF+Thumb+Thumb
5,10-Methenyltetrahydrofolic acid + NADPH + NADPH → 5,10-Methylene-THF + NADP + 5,10-Methylene-THF
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PseudoCyc/BioCyc Reactions:
Thumb+Thumb ↔ Thumb+Thumb
Water + 5,10-Methenyltetrahydrofolate ↔ N10-Formyl-THF + Hydrogen ion
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Thumb+Thumb ↔ Thumb+Thumb+Thumb
5,10-Methylene-THF + NADP ↔ 5,10-Methenyltetrahydrofolate + NADPH + Hydrogen ion
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Complex Reactions:
Thumb+Thumb → Thumb+Thumb
5,10-Methylene-THF + NADP → 5,10-Methenyltetrahydrofolate + NADPH
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Thumb+Thumb → Thumb
5,10-Methenyltetrahydrofolate + Water → N10-Formyl-THF
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0015855,10-MethenyltetrahydrofolateMetaboCard
PAMDB0003535,10-Methylene-THFMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000213N10-Formyl-THFMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
binding
catalytic activity
Process
cellular aromatic compound metabolic process
cellular metabolic process
folic acid and derivative biosynthetic process
folic acid and derivative metabolic process
metabolic process
Gene Properties
Locus tag: PA1796
Strand: -
Entrez Gene ID: 880636
Accession: NP_250487.1
GI: 15596993
Sequence start: 1946187
Sequence End: 1947041
Sequence Length: 854
Gene Sequence: 
>PA1796
ATGACCGCACAACTGATCGACGGCAAAGCGATCGCCGCCAACCTTCGCCAGCAGATAGCCCAACGCGTGACCGAGCGCCGCCAGCAAGGCCTGCGCGTTCCCGGCCTGGCGGTGATCCTGGTCGGCACCGATCCGGCCTCTCAGGTCTATGTGGCGCACAAGCGCAAGGACTGCGAGGAAGTCGGCTTTCTCTCCCAGGCCTACGATCTTCCCGCCGAAACCAGCCAGGACGACCTGCTGGCCCTGATCGACCGCCTGAACGACGATCCCGCCATCGACGGCATCCTGGTCCAGCTACCCCTGCCCGCCCACCTGGACGCCTCCCTGCTGCTGGAGCGTATCCACCCGGACAAGGACGTGGACGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAAGGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTCGAACATCGTCGGCCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCACCGTCACCGTGACCCACCGCTTCACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTCAAGGGCGAGTGGATCAAGGAAGGCGCCATCGTCATCGACGTCGGCATCAACCGCCAGGCCGACGGCCGCCTGGTCGGCGACGTGGAATACGAGGTGGCGGCACAACGCGCCAGCTGGATCACCCCGGTGCCGGGCGGCGTCGGGCCGATGACCCGCGCCTGCCTGCTGGAAAATACCCTGCACGCCGCCGAACACCTGCACGACTGA
Protein Properties
Protein Residues: 284
Protein Molecular Weight: 30.5 kDa
Protein Theoretical pI: 5.8
Hydropathicity (GRAVY score): 0.053
Charge at pH 7 (predicted): -5.96
Protein Sequence: 
>PA1796
MTAQLIDGKAIAANLRQQIAQRVTERRQQGLRVPGLAVILVGTDPASQVYVAHKRKDCEEVGFLSQAYDLPAETSQDDLLALIDRLNDDPAIDGILVQLPLPAHLDASLLLERIHPDKDVDGFHPYNIGRLAQRMPLLRPCTPKGIMTLLASTGADLYGMDAVVVGASNIVGRPMALELLLGGCTVTVTHRFTRDLADHVSRADLVVVAAGKPGLVKGEWIKEGAIVIDVGINRQADGRLVGDVEYEVAAQRASWITPVPGGVGPMTRACLLENTLHAAEHLHD
References
External Links:
Resource Link
Genome ID: PA1796
Entrez Gene ID: 880636
NCBI Protein ID: 15596993
General Reference: PaperBLAST - Find papers about PA1796 and its homologs