Nitrite reductase [NAD(P)H] large subunit (PA1781)

Identification
Name: Nitrite reductase [NAD(P)H] large subunit
Synonyms: Not Available
Gene Name: nirB
Enzyme Class:
Biological Properties
General Function: Involved in oxidation-reduction process
Specific Function: Ammonium hydroxide + 3 NAD(P)(+) + H(2)O = nitrite + 3 NAD(P)H
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+3 Thumb+2 Thumb ↔ Thumb+Thumb+3 Thumb+5 Thumb
Ammonia + NAD + 3 NADP + 2 Water ↔ Nitrite + NADH + 3 NADPH + 5 Hydrogen ion
ReactionCard
SMPDB Reactions:
Nitrite+3 Thumb+5 Thumb+Thumb ? Thumb+2 Thumb+3 Thumb
Nitrite + 3 NADH + 5 Hydrogen ion + Nitrite ? Ammonia + 2 Water + 3 NAD
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+3 Thumb+2 Thumb ↔ Thumb+Thumb+3 Thumb+5 Thumb
Ammonia + NAD + 3 NADP + 2 Water ↔ Nitrite + NADH + 3 NADPH + 5 Hydrogen ion
ReactionCard
Nitrite+3 Thumb+5 Thumb+Thumb ? Thumb+2 Thumb+3 Thumb
Nitrite + 3 NADH + 5 Hydrogen ion + Nitrite ? Ammonia + 2 Water + 3 NAD
ReactionCard
Thumb
2 nitric oxide + an oxidized unknown electron acceptor + 2 H2O = 2 nitrite + an reduced unknown electron acceptor
ReactionCard
Complex Reactions:
5 Thumb+3 Thumb+Thumb → 2 Thumb+3 Thumb+Thumb
5 Hydrogen ion + 3 NADH + Nitrite → 2 Water + 3 NAD + Ammonium
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000017AmmoniaMetaboCard
PAMDB001595AmmoniumMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
PAMDB000465NitriteMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
binding
catalytic activity
cation binding
FAD or FADH2 binding
heme binding
ion binding
iron ion binding
iron-sulfur cluster binding
metal cluster binding
metal ion binding
NADP or NADPH binding
nitrite reductase [NAD(P)H] activity
nucleoside binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on other nitrogenous compounds as donors
oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
purine nucleoside binding
transition metal ion binding
Process
metabolic process
nitrate assimilation
nitrate metabolic process
nitrogen compound metabolic process
oxidation reduction
Gene Properties
Locus tag: PA1781
Strand: -
Entrez Gene ID: 877714
Accession: NP_250472.1
GI: 15596978
Sequence start: 1926176
Sequence End: 1928626
Sequence Length: 2450
Gene Sequence: 
>PA1781
ATGAAGAAGATCAAGCTGGTGATGGTCGGCAACGGCATGGCCGGGGTCCGTACCCTGGAAGAACTGCTCAAGCTCAACTCGGACTTCTACGACATCACGGTGTTCGGCGCCGAGCCCCATCCCAACTACAACCGCATCCTGCTGTCCCCGGTGCTGGCCGGCGAGCAGACCTTCGAGGAAATCGTCCTCAACGACCTCAACTGGTATGCGGAAAACGGCATCAAGCTCCTCCTCGACCGCAAGGTGGTGCAGATCGACCGGCTTCGCCGCAGGGTGGTCGCCGCGGACGGCAGCGAGGCCGAGTACGACCGCCTGCTGCTGGCCACCGGCTCGCTCCCCTTCATCCTGCCGATACCCGGCAATCGCCTGCAGGGCGTGATCGGCTACCGCGACATCGCCGACACCCAGGCCATGATCGACTGCGCCCGGACCCACAGCCACGCGGTGGTGATCGGCGGCGGCCTGCTCGGCCTGGAGGCGGCCAACGGCCTCAAGCAACGCGGCATGGACGTGACCGTCGTGCACCTCTCCGACTGGCTGCTGGAACGCCAACTGGACCGCACCGCCGGCAAACTCCTGCAAGGCGCGCTGGAAGCCCGCGGCATCCGCTTCCGCCTGAACACCCAGACCCAGGAACTGATGGACAACGGCAGCGGCCGGGTCTGTGCCGTGCAGTTCAACGACGGCGACGTGATTCCCGCCGACCTGGTGGTAATGGCCGCCGGCATCCGCCCCAATACCGAGCTGGCCGAAAGCGCGGGCATCCCCTGCAACCGCGGCATCCTGGTCAACGACACCCTGCAGACCTACGACCCACGCATCTATGCGGTCGGCGAATGCGCCAACCACCGTGGCATCGCCTACGGCCTGGTTGCGCCGCTGTTCGAGCAGGCCAAGGTCTGCGCCAACCACCTGGCTCATCTCGGCTACGCCCGCTACCAGGGCTCGGTGACCTCGACCAAGCTCAAGGTGACCGGTATCGACCTGTTCTCCGCCGGCGACTTCATCGGCGGCGAAGGCAGCGAGACCATCACCCTTTCCGACCCCATCGGCGGCGTCTACAAGAAGCTGGTGATCAAGGACGATGTACTGGTCGGCGCCTGTCTCTACGGCGACACCGCCGACGGCGGCTGGTACTTCCGGCAGATCCGCGAGAACCACAACGTCGCGCAGATCCGCGACCACCTGATGTTCGGCGAGAACGCCCTCGGCGATGTCGGCCACCAGGGCCAGAACAGCGCCGCGAGCATGCCCGACACGGCCGAAGTATGTGGCTGCAACGGCGTCTGCAAGGGCACCATCGTCAAGGCCATACAAGAACACGGCCTGTTCAGCGTCGACGAAGTCAAGAAACACACCAAGGCCGCCAGCTCCTGCGGCTCCTGCGCGGGATTGGTGGAACAGATCCTGATCAGCACCGTCGGCGGCGCCGCCGACGTCAAGCCGAAGAGCGAGAAGGCCATCTGCGGCTGCAGCGACCTCAACCACGGGCAGATCCGCAAGGCCATTCGCGAACACCGGCTGACCAGTCTCGACGCCGCCATGCGCTTCATGGACTGGAAGACCCCGAACGGCTGTGCCACCTGCCGCCCGGCCCTGAACTACTACCTGATCTCGACCTGGCCGGGAGAAGCCAAGGACGATCCGCAGTCGCGCCTGATCAACGAACGCGCCCACGCCAACATCCAGAAGGACGGCACCTACTCGGTGGTACCGCGGATGTGGGGCGGCGTGACCAACGCCGCCGAGTTGCGGCGCATCGCCGACGTCGCCGACAAGTACCAGGTGCCGATGGTCAAGGTCACCGGCGGCCAGCGCATCGACCTGCTGGGGGTGAAGAAGGACGACCTGCCGGCCATCTGGAAAGACCTCGACATGCCCTCCGGACATGCCTACGGCAAATCCATCCGCACCGTGAAGACCTGTGTCGGCAGCGAATTCTGCCGCTTCGGCACGCAGAACTCGACGCAGCTGGGCATCGATCTGGAACACGACCTGTTCAACATGTGGTCGCCGCACAAGGTCAAGCTGGCGGTCTCCGGCTGCCCGCGCAATTGCGCCGAGGCCGGGATCAAGGACGTCGGCATCATCGGCGTGGACTCCGGCTGGGAGATGTACATCGGCGGCAACGGCGGGATCAAGACCGAGGTCGCGGAGTTCTTCGTCAAGCTCAAGACCGCCGAAGAGGTGCGCGAATACAACGGCGCCTTCCTCCAGCTCTACCGCGAGGAAGCCTTCTACCTGGAGCGCACGGTGCATTATCTGCAGCGGGTCGGCATGGAGCACATCCGCAAGGCGGTGGTCGAGGACGCGGAAAACCGCAAGGCGCTCAACGACCGCCTGCAATTCGCCCTGTCCTTCGAGCAGGACCCATGGAAGCAGCGCATCGAACAGCCGCAATTGAAGAAGGAGTTCGAACGGATTTCGCTCAAGCAACTGGAAAACGTCTGA
Protein Properties
Protein Residues: 816
Protein Molecular Weight: 89.7 kDa
Protein Theoretical pI: 6.75
Hydropathicity (GRAVY score): -0.21
Charge at pH 7 (predicted): -2.72
Protein Sequence: 
>PA1781
MKKIKLVMVGNGMAGVRTLEELLKLNSDFYDITVFGAEPHPNYNRILLSPVLAGEQTFEEIVLNDLNWYAENGIKLLLDRKVVQIDRLRRRVVAADGSEAEYDRLLLATGSLPFILPIPGNRLQGVIGYRDIADTQAMIDCARTHSHAVVIGGGLLGLEAANGLKQRGMDVTVVHLSDWLLERQLDRTAGKLLQGALEARGIRFRLNTQTQELMDNGSGRVCAVQFNDGDVIPADLVVMAAGIRPNTELAESAGIPCNRGILVNDTLQTYDPRIYAVGECANHRGIAYGLVAPLFEQAKVCANHLAHLGYARYQGSVTSTKLKVTGIDLFSAGDFIGGEGSETITLSDPIGGVYKKLVIKDDVLVGACLYGDTADGGWYFRQIRENHNVAQIRDHLMFGENALGDVGHQGQNSAASMPDTAEVCGCNGVCKGTIVKAIQEHGLFSVDEVKKHTKAASSCGSCAGLVEQILISTVGGAADVKPKSEKAICGCSDLNHGQIRKAIREHRLTSLDAAMRFMDWKTPNGCATCRPALNYYLISTWPGEAKDDPQSRLINERAHANIQKDGTYSVVPRMWGGVTNAAELRRIADVADKYQVPMVKVTGGQRIDLLGVKKDDLPAIWKDLDMPSGHAYGKSIRTVKTCVGSEFCRFGTQNSTQLGIDLEHDLFNMWSPHKVKLAVSGCPRNCAEAGIKDVGIIGVDSGWEMYIGGNGGIKTEVAEFFVKLKTAEEVREYNGAFLQLYREEAFYLERTVHYLQRVGMEHIRKAVVEDAENRKALNDRLQFALSFEQDPWKQRIEQPQLKKEFERISLKQLENV
References
External Links:
Resource Link
Genome ID: PA1781
Entrez Gene ID: 877714
NCBI Protein ID: 15596978
General Reference: PaperBLAST - Find papers about PA1781 and its homologs