Oxygen-independent coproporphyrinogen-III oxidase (PA1546)

Identification
Name: Oxygen-independent coproporphyrinogen-III oxidase
Synonyms:
  • Coprogen oxidase
  • Coproporphyrinogenase
Gene Name: hemN
Enzyme Class:
Biological Properties
General Function: Involved in coproporphyrinogen oxidase activity
Specific Function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX
Cellular Location: Cytoplasm
KEGG Pathways:
  • Porphyrin and chlorophyll metabolism pae00860
KEGG Reactions:
2 Thumb+Thumb ↔ 2 Thumb+2 Thumb+2 Thumb+Thumb
2 S-Adenosylmethionine + Coproporphyrin III ↔ 2 Carbon dioxide + 2 5'-Deoxyadenosine + 2 L-Methionine + Protoporphyrinogen IX
ReactionCard
Thumb+2 Thumb ↔ Thumb+2 Thumb+2 Thumb+2 Thumb
Coproporphyrin III + 2 S-Adenosylmethionine ↔ Protoporphyrinogen IX + 2 Carbon dioxide + 2 L-Methionine + 2 5'-Deoxyadenosine
ReactionCard
SMPDB Reactions:
S-adenosyl-L-methionine+Thumb → Thumb+Thumb+Thumb+Thumb
S-adenosyl-L-methionine + Coproporphyrinogen III → 5'-Deoxyadenosine + L-Methionine + Carbon dioxide + Protoporphyrinogen IX
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb+Thumb
Coproporphyrinogen III + S-Adenosylmethionine → Protoporphyrinogen IX + Carbon dioxide + L-Methionine + 5'-Deoxyadenosine
ReactionCard
Complex Reactions: Not Available
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0015835'-DeoxyadenosineMetaboCard
PAMDB000568Carbon dioxideMetaboCard
PAMDB000151Coproporphyrin IIIMetaboCard
PAMDB000314Coproporphyrinogen IIIMetaboCard
PAMDB000175L-MethionineMetaboCard
PAMDB000254Protoporphyrinogen IXMetaboCard
PAMDB000289S-AdenosylmethionineMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
binding
catalytic activity
coproporphyrinogen oxidase activity
iron-sulfur cluster binding
metal cluster binding
oxidoreductase activity
oxidoreductase activity, acting on the CH-CH group of donors
oxidoreductase activity, acting on the CH-CH group of donors, oxygen as acceptor
Process
metabolic process
nitrogen compound metabolic process
oxidation reduction
porphyrin biosynthetic process
porphyrin metabolic process
tetrapyrrole metabolic process
Gene Properties
Locus tag: PA1546
Strand: -
Entrez Gene ID: 883053
Accession: NP_250237.1
GI: 15596743
Sequence start: 1682567
Sequence End: 1683949
Sequence Length: 1382
Gene Sequence: 
>PA1546
ATGCTCGACACCATTCGCTGGGACGCTGACCTGATTCGGCGCTACGACCTTTCCGGCCCGCGTTACACCTCCTATCCGACCGCCGTGCAGTTCCACGAAGGCATCGGCCCGTTCGACCAGTTGCACGCCCTGCGCGACAGCCGGAAGGCCGGACACCCCCTGTCGCTCTACGTGCACATCCCGTTCTGCGCCAACATCTGCTACTACTGCGCCTGCAACAAGGTGATCACCAAGGATCGCGGGCGCAGCGCGCCGTACCTTGCGCGCCTGGTCCGCGAGATCGAGATCGTCAGCCGCCACCTCTCCCGCGAGCAGGTGGTCGAGCAACTGCACTTCGGTGGCGGAACCCCGACCTTCCTCAGTCCCGGCCAGTTGCGCGAACTCATGTCGCAATTGCGCACCCACCTCAACCTGCTGGACGACGATTCGGGCGACTACGGCATCGAGATCGACCCGCGCGAGGCCGACTGGTCGACCATGGGCCTGCTCCGCGAACTCGGTTTCAACCGCGTCAGCCTCGGCGTACAGGACTTCGACATGGAGGTGCAGAAGGCGGTCAACCGCATGCAGACCCCGGAGGAGACCCGTACCATCGTCGAGGCCGCGCGCACCCTGCAATACCGCTCGATCAACCTCGACCTGATCTACGGACTGCCGAAACAGACGCCGGACAGCTTCGCCCGCACGGTCGACGAAGTGATCGCGCTACAGCCGGATCGCCTCTCGGTGTTCAACTACGCCCACCTGCCCGAGCGTTTCATGCCGCAGCGGCGGATCAACGCCGACGACCTGCCGAGCCCGGGCCAGAAGCTGGAAATGCTCCAGCGCACCACCGAGCAACTCGCCGCGGCGGGCTACCGCTATATCGGCATGGATCACTTCGCCCTACCCGACGACGAGCTGGCCAGCGCCCAGGAAGACGGCACCCTGCAACGCAATTTCCAGGGCTACACCACGCACGGGCACTGTGACCTGGTAGGTCTCGGGGTCTCGGCGATCAGCCAGATCGGCGACCTCTACAGCCAGAACAGCAGCGACATCAACGACTACCAGACCAGCCTCGACAACGGCCAGTTGGCGATCCGTCGCGGCCTGCACTGCAACAGCGACGACCGGGTGCGGCGCGCGGTGATCCAGCAACTGATCTGCCACTTCGAACTGGCCTTCGAGGATATCGAGACAGAGTTCGGCATCGACTTCCGCAGTTACTTCGCCGAGCTGTGGCCCGATCTCGAACGCTTCGCCGCCGACGGCCTGATCCGCCTGGACGCCAAGGGCATCGACATCACGTCTTCCGGACGTCTGCTGGTGCGTTCGATCTGCATGCTGTTCGACCGCTACCTGCCCAGCCTCAACCGGCAGCGCTTCTCCCGGGTGATCTGA
Protein Properties
Protein Residues: 460
Protein Molecular Weight: 52.5 kDa
Protein Theoretical pI: 5.46
Hydropathicity (GRAVY score): -0.367
Charge at pH 7 (predicted): -11.1
Protein Sequence: 
>PA1546
MLDTIRWDADLIRRYDLSGPRYTSYPTAVQFHEGIGPFDQLHALRDSRKAGHPLSLYVHIPFCANICYYCACNKVITKDRGRSAPYLARLVREIEIVSRHLSREQVVEQLHFGGGTPTFLSPGQLRELMSQLRTHLNLLDDDSGDYGIEIDPREADWSTMGLLRELGFNRVSLGVQDFDMEVQKAVNRMQTPEETRTIVEAARTLQYRSINLDLIYGLPKQTPDSFARTVDEVIALQPDRLSVFNYAHLPERFMPQRRINADDLPSPGQKLEMLQRTTEQLAAAGYRYIGMDHFALPDDELASAQEDGTLQRNFQGYTTHGHCDLVGLGVSAISQIGDLYSQNSSDINDYQTSLDNGQLAIRRGLHCNSDDRVRRAVIQQLICHFELAFEDIETEFGIDFRSYFAELWPDLERFAADGLIRLDAKGIDITSSGRLLVRSICMLFDRYLPSLNRQRFSRVI
References
External Links:
Resource Link
Genome ID: PA1546
Entrez Gene ID: 883053
NCBI Protein ID: 15596743
General Reference: PaperBLAST - Find papers about PA1546 and its homologs