| Identification |
| Name: |
Prolyl-tRNA synthetase |
| Synonyms: |
- Global RNA synthesis factor
- Proline--tRNA ligase
- ProRS
|
| Gene Name: |
proS |
| Enzyme Class: |
|
| Biological Properties |
| General Function: |
Involved in nucleotide binding |
| Specific Function: |
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). Misacylated Cys-tRNA(Pro) is not edited by ProRS, but instead may be edited in trans by ybaK |
| Cellular Location: |
Cytoplasm |
| KEGG Pathways: |
|
| KEGG Reactions: |
|
 | + |  | + | tRNA(Pro) | + | tRNA(Pro) | ↔ |  | + |  | + | L-Prolyl-tRNA(Pro) | + |  |
| | |
| + | | + | tRNA(Pro) | ↔ | | + | | + | L-Prolyl-tRNA(Pro) |
| |
|
| SMPDB Reactions: |
|
L-Proline | + | | + |  | + | tRNA(Pro) | + | | → | | + | Pyrophosphate | + | L-prolyl-tRNA(Pro) |
| |
|
| PseudoCyc/BioCyc Reactions: |
|
| Complex Reactions: |
|
| + | | + | tRNA(Pro) | → | | + | | + | L-prolyl-tRNA(Pro) |
| |
|
| Transports: |
Not Available |
| Metabolites: |
|
| GO Classification: |
| Component |
|---|
| cell part | | cytoplasm | | intracellular part | | Function |
|---|
| adenyl nucleotide binding | | adenyl ribonucleotide binding | | aminoacyl-tRNA ligase activity | | ATP binding | | binding | | catalytic activity | | ligase activity | | ligase activity, forming aminoacyl-tRNA and related compounds | | ligase activity, forming carbon-oxygen bonds | | nucleoside binding | | nucleotide binding | | proline-tRNA ligase activity | | purine nucleoside binding | | Process |
|---|
| biosynthetic process | | cellular macromolecule biosynthetic process | | cellular macromolecule metabolic process | | macromolecule biosynthetic process | | macromolecule metabolic process | | metabolic process | | ncRNA metabolic process | | prolyl-tRNA aminoacylation | | RNA metabolic process | | translation | | tRNA aminoacylation | | tRNA aminoacylation for protein translation | | tRNA metabolic process |
|
| Gene Properties |
| Locus tag: |
PA0956 |
| Strand: |
- |
| Entrez Gene ID: |
879563 |
| Accession: |
NP_249647.1 |
| GI: |
15596153 |
| Sequence start: |
1041689 |
| Sequence End: |
1043404 |
| Sequence Length: |
1715 |
| Gene Sequence: |
>PA0956
ATGCGTACCAGTCAGTATCTGCTTTCCACGCTCAAAGAAACCCCCGCCGACGCGGTGGTAATCAGCCATCAACTGCTGCTTCGCGCCGGCATGATCCGTCGCCTGGCCTCGGGTCTCTACACCTGGCTGCCAATGGGCCTGCGGGTACTGCGCAAGGTCGAGACCATCGTCCGCGAGGAAATGAACGCTGCCGGCGCCCTGGAAGTGCTGATGCCGGCGGTGCAGCCGGCCGAGCTGTGGCAGGAGTCGGGGCGCTGGGAGCAATACGGCCCTGAGCTGCTGCGCCTGAAGGACCGCCACGAGCGCGAATTCTGCGTAGGCCCGACCCACGAAGAAGTGATCACCGACCTCGCGCGCAACGAGCTGAACAGCTACAAGCAACTGCCGATCAACTTCTATCAGATCCAGACCAAGTTCCGCGATGAGATCCGCCCGCGCTTCGGCCTGATGCGCGGACGCGAATTCATCATGAAGGACGCCTATTCCTTCCACCTGAGCCAGGACTCGCTGCAGCAGACCTACGACGGGATGTACCAGGCGTACAGCAAGATCTTCTCCCGCCTCGGCCTGGATTTCCGTCCCGTGCAAGCCGACAACGGCTCCATCGGCGGCAGCGGCTCCCATGAGTTCCACGTGCTGGCCAACTCCGGCGAGGACGATATCGTCTTCAGCGACAGCTCCGACTACGCCGCGAACATCGAGAAGGCCGAAGCCGTGCCGCGCGAGAGCGCCCGCGGCAGCGCCACCGAGGACATGCGCCTGGTCGACACGCCCAACACCAAGACCATCGCGGCCCTGGTGGACGGCTTCCAGCTACCGATCGAGAAAACCATCAAGACCCTGGTGGTGCATGGCGCCGAAGAAGGCACCCTGGTCGCCCTGATCGTCCGCGGTGATCACGAGCTCAACGAAATCAAGGCCGCCAACCAGCCGCTGGTCGCCAGCCCGCTGGTATTCGCTTCGGAGGCCGAGATCCGTGCCGCCATCGGTGCCGGCCCCGGCTCGCTGGGCCCGGTCAACCTGCCGATCGCCTGCATCGTCGACCGCTCCGTCGCCCTGATGAGCGATTTCGCCGCTGGCGCCAACATCGAGGACAAGCACTACTTCGGCGTGAACTGGGAGCGCGACCTGCCGCTGCCCGAGGTCGCCGACCTGCGCAATGTCGTCGAAGGCGACCCCAGCCCGGACGGCAAGGGCACCCTGGTGATCAAGCGCGGCATCGAGGTCGGTCATATCTTCCAGCTTGGCACCAAGTACAGCGAAGCGATGAAGCTGAGCGTCCTGAGCGAACAGGGCAAGCCGGTCAACCTGATCATGGGCTGCTACGGCATCGGCGTATCCCGCGTGGTCGCCGCCGCCATCGAGCAGAACCATGACGAGCGCGGCATTCTCTGGCCAAGCGCATTGGCGCCGTTCCAGATCGCCCTGGTACCGCTGAAGTACGAAACCGAGAGCGTCAAGCAGGCGACCGACAAGCTCTATGCCGAGCTGACCGCAGCCGGTTTCGAAGTGCTGCTGGACGATCGCGACAAGAAGACCAGCCCCGGGGTGAAGTTCGCCGACATGGAGCTGATCGGCATTCCCCACCGCATCGTGATCAGCGACCGCGGCCTCAGCGAAGGCGTCCTGGAATACAAGGGCCGCCGCGACAGCGAGTCGCAGAACCTGCCGATCGGCGAACTGATGTCGTTCATCACCGAAAAACTCAGCCGCTGA |
| Protein Properties |
| Protein Residues: |
571 |
| Protein Molecular Weight: |
63.1 kDa |
| Protein Theoretical pI: |
5.09 |
| Hydropathicity (GRAVY score): |
-0.194 |
| Charge at pH 7 (predicted): |
-15.16 |
| Protein Sequence: |
>PA0956
MRTSQYLLSTLKETPADAVVISHQLLLRAGMIRRLASGLYTWLPMGLRVLRKVETIVREEMNAAGALEVLMPAVQPAELWQESGRWEQYGPELLRLKDRHEREFCVGPTHEEVITDLARNELNSYKQLPINFYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFHLSQDSLQQTYDGMYQAYSKIFSRLGLDFRPVQADNGSIGGSGSHEFHVLANSGEDDIVFSDSSDYAANIEKAEAVPRESARGSATEDMRLVDTPNTKTIAALVDGFQLPIEKTIKTLVVHGAEEGTLVALIVRGDHELNEIKAANQPLVASPLVFASEAEIRAAIGAGPGSLGPVNLPIACIVDRSVALMSDFAAGANIEDKHYFGVNWERDLPLPEVADLRNVVEGDPSPDGKGTLVIKRGIEVGHIFQLGTKYSEAMKLSVLSEQGKPVNLIMGCYGIGVSRVVAAAIEQNHDERGILWPSALAPFQIALVPLKYETESVKQATDKLYAELTAAGFEVLLDDRDKKTSPGVKFADMELIGIPHRIVISDRGLSEGVLEYKGRRDSESQNLPIGELMSFITEKLSR |
| References |
| External Links: |
|
| General Reference: |
PaperBLAST - Find papers about PA0956 and its homologs
|
