Thymidylate synthase (PA0342)

Identification
Name: Thymidylate synthase
Synonyms:
  • TS
  • TSase
Gene Name: thyA
Enzyme Class:
Biological Properties
General Function: Involved in thymidylate synthase activity
Specific Function: Provides the sole de novo source of dTMP for DNA biosynthesis. This protein also binds to its mRNA thus repressing its own translation
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb
dUMP + 5,10-Methylene-THF ↔ Dihydrofolic acid + 5-Thymidylic acid
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
dUMP + 5,10-Methylene-THF ↔ Dihydrofolic acid + 5-Thymidylic acid
ReactionCard
SMPDB Reactions:
Thumb+Thumb+Dihydrofolic acid → 5,10-Methylene-THF+Thumb+Thumb
Dihydrofolic acid + 5-Thymidylic acid + Dihydrofolic acid → 5,10-Methylene-THF + dUMP + 5,10-Methylene-THF
ReactionCard
Thumb+5,10-methenyltetrahydrofolate mono-L-glutamate+5,10-methenyltetrahydrofolate mono-L-glutamate → Thumb+Thumb+Dihydrofolic acid
dUMP + 5,10-methenyltetrahydrofolate mono-L-glutamate + 5,10-methenyltetrahydrofolate mono-L-glutamate → Dihydrofolic acid + 5-Thymidylic acid + Dihydrofolic acid
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb ↔ Thumb+Thumb
dUMP + 5,10-Methylene-THF ↔ Dihydrofolic acid + 5-Thymidylic acid
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Complex Reactions:
Thumb+Thumb → Thumb+Thumb
5,10-Methylene-THF + dUMP → Dihydrofolic acid + 5-Thymidylic acid
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0003535,10-Methylene-THFMetaboCard
PAMDB0003035-Thymidylic acidMetaboCard
PAMDB000235Dihydrofolic acidMetaboCard
PAMDB000375dUMPMetaboCard
GO Classification:
Function
5,10-methylenetetrahydrofolate-dependent methyltransferase activity
catalytic activity
methyltransferase activity
thymidylate synthase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular nitrogen compound metabolic process
dTMP biosynthetic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
pyrimidine deoxyribonucleoside monophosphate biosynthetic process
pyrimidine nucleoside monophosphate biosynthetic process
pyrimidine nucleotide biosynthetic process
pyrimidine nucleotide metabolic process
Gene Properties
Locus tag: PA0342
Strand: +
Entrez Gene ID: 879417
Accession: NP_249033.1
GI: 15595539
Sequence start: 384733
Sequence End: 385527
Sequence Length: 794
Gene Sequence: 
>PA0342
ATGAAACAGTACCTCGACCTGATGCGCCACGTGCGCGAGCACGGCACCTTCAAGAGCGACCGCACCGGCACCGGCACCTATAGCGTGTTCGGCCACCAGATGCGCTTCGACCTGGCCGCGGGCTTCCCCCTGGTGACCACCAAGAAGTGCCACCTCAAGTCGATCGTCCACGAGCTGCTGTGGTTCCTCAAGGGCTCCACCAATATCGCCTACCTCAAGGAGCACGGCGTCTCGATCTGGGACGAATGGGCCGACGAGAACGGCGACCTCGGCCCGGTGTATGGCTACCAGTGGCGCTCCTGGCCGGCGCCGGACGGCCGCCACATCGACCAGATCGCCAACCTGATGGCGATGCTGAAGAAGAATCCGGACTCGCGCCGGCTGATCGTCTCCGCCTGGAACCCGGCGCTGATCGACGAGATGGCCCTGCCGCCCTGCCACGCGCTGTTCCAGTTCTACGTCGCCGACGGCAAGCTCAGCTGCCAGCTCTACCAACGTTCGGCGGACATCTTCCTCGGCGTACCCTTCAATATCGCCAGCTATGCCCTGCTGACCCTGATGGTGGCGCAGGTCGCCGGCCTGCAGCCGGGCGAGTTCATCTGGACCGGCGGCGATTGCCACCTGTACGCCAACCACCTGGAGCAGGCCGACCTGCAGCTGACCCGCGAGCCGCTGCCGTTGCCGAGCATGAAGCTGAATCCCGAGGTGAAGGATCTGTTCGACTTTCGCTTCGAGGACTTCGAGCTGGTCGGCTACGAGGCCCATCCGCACATCAAGGCACCGGTCGCGGTCTGA
Protein Properties
Protein Residues: 264
Protein Molecular Weight: 30 kDa
Protein Theoretical pI: 6.51
Hydropathicity (GRAVY score): -0.154
Charge at pH 7 (predicted): -3.24
Protein Sequence: 
>PA0342
MKQYLDLMRHVREHGTFKSDRTGTGTYSVFGHQMRFDLAAGFPLVTTKKCHLKSIVHELLWFLKGSTNIAYLKEHGVSIWDEWADENGDLGPVYGYQWRSWPAPDGRHIDQIANLMAMLKKNPDSRRLIVSAWNPALIDEMALPPCHALFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTLMVAQVAGLQPGEFIWTGGDCHLYANHLEQADLQLTREPLPLPSMKLNPEVKDLFDFRFEDFELVGYEAHPHIKAPVAV
References
External Links:
Resource Link
Genome ID: PA0342
Entrez Gene ID: 879417
NCBI Protein ID: 15595539
General Reference: PaperBLAST - Find papers about PA0342 and its homologs