REcut 3.0

Automatic Restriction Enzyme Selector for DNA shuffling

part of Re_cutX_over - A suite of computaional tools for accelerating the design, visualization and analysis of recombinatorial protein libraries

Please paste parent sequences in FASTA format:

>2A5
ATGCTGACCTCAGGACTCCTCCTGGTGGCTGCAGTGGCCTTCCTCAGCGTCCTGGTCTTGATGTCTGTCTGGAAGCAGAGAAAGCTCTCAGGAAAGCTGCCTCCAGGACCCACCCCACTGCCCTTCATTGGGAACTTCCTTCAGCTGAACACAGAGCAAATGTACAACTCTCTCATGAAGATCAGCCAACGTTATGGTCCTGTATTCACCATCTACCTGGGACCTCGCCGAATTGTGGTGCTGTGCGGACAGGAGGCAGTCAAGGAAGCTCTGGTGGACCAAGCTGAGGAATTCAGCGGGCGGGGCGAGCAAGCTACCTTCGACTGGCTTTTCAAAGGCTATGGCGTAGTCTTCAGCAGCGGGGAGCGAGCCAAACAGCTAAGGCGCTTCTCCATCGCCACGCTGCGGGACTTCGGCGTGGGGAAGCGTGGCATCGAGGAGCGCATCCAAGAGGAGGCGGGCTTTCTCATCGATTCATTTCGGAAGACGAACGGTGCTTTTATTGACCCCACCTTCTACCTTAGCCGAACAGTCTCCAATGTCATTAGCTCAATTGTCTTCGGGGACCGCTTTGACTATGAGGACAAAGAGTTCCTGTCACTGCTTCGAATGATGCTGGGAAGCTTCCAGTTCACTGCTACCTCCATGGGGCAGCTCTATGAGATGTTCTCTTCTGTGATGAAACACCTGCCAGGGCCCCAGCAACAGGCCTTTAAGGAGCTGCAGGGCCTGGAGGACTTCATAACCAAGAAAGTGGAACACAATCAGCGCACGCTGGATCCCAATTCCCCAAGGGACTTCATCGACTCCTTCCTCATCCGAATGCTGGAGGAGAAGAAGAACCCCAATACTGAGTTCTACATGAAGAACTTGGTGCTGACTACACTAAATCTCTTCTTTGCTGGCACAGAGACCGTCAGCACCACCCTGCGCTATGGCTTTCTGTTGCTCATGAAGCACCCAGATATTGAGGCCAAGGTCCATGAGGAGATTGATCGGGTGATTGGCAGGAACCGGCAGCCCAAGTATGAGGACCGAATGAAGATGCCCTATACGGAGGCTGTAATCCATGAGATCCAGAGATTTGCAGACATGATCCCCATGGGCCTGGCTCGAAGGGTCACCAAGGACACCAAGTTTCGAGATTTCCTCCTCCCCAAGGGTACTGAAGTGTTTCCTATGCTGGGCTCTGTGCTGAAAGACCCCAAGTTCTTCTCCAACCCCAAAGACTTCAACCCAAAGCACTTCCTAGATGACAAGGGACAGTTTAAGAAGAATGATGCCTTTGTGCCCTTTTCCATTGGAAAACGGTATTGTTTCGGAGAAGGACTGGCTAGGATGGAACTCTTCCTCTTCCTCACAAACATCATGCAGAACTTCCACTTCAAATCCACACAGGCACCCCAGGACATCGATGTGTCTCCTAGACTCGTGGGCTTTGCCACGATCCCACCAACCTACACTATGAGTTTCTTGTCCCGTGCGTCGACCCATCATCATCATCATCATTGA
>2A6
ATGGCTGCTTCAGGAATGTTATTAGTGGCCTTGCTGGTCTGCCTGACTGTGATGGTCTTGATGTCTGTTTGGCAGCAGAGGAAGAGCAAGGGGAAGCTGCCTCCGGGACCCACCCCATTGCCCTTCATTGGAAACTACCTGCAGCTGAACACAGAGCAGATGTACAACTCCCTCATGAAGATCAGTGAGCGCTATGGCCCCGTGTTCACCATTCACTTGGGGCCCCGGCGGGTCGTGGTGCTGTGTGGACATGATGCCGTCAGGGAGGCTCTGGTGGACCAGGCTGAGGAGTTCAGCGGGCGAGGCGAGCAAGCCACCTTCGACTGGGTCTTCAAAGGCTATGGCGTGGTATTCAGCAACGGGGAGCGCGCCAAGCAGCTCCGGCGCTTCTCCATCGCCACCCTGCGGGACTTCGGGGTGGGCAAGCGAGGCATCGAGGAGCGCATCCAGGAGGAGGCGGGCTTCCTCATCGACGCCCTCCGGGGCACTGGCGGCGCCAATATCGATCCCACCTTCTTCCTGAGCCGCACAGTCTCCAATGTCATCAGCTCCATTGTCTTTGGGGACCGCTTTGACTATAAGGACAAAGAGTTCCTGTCACTGTTGCGCATGATGCTAGGAATCTTCCAGTTCACGTCAACCTCCACGGGGCAGCTCTATGAGATGTTCTCTTCGGTGATGAAACACCTGCCAGGACCACAGCAACAGGCCTTTCAGTTGCTGCAAGGGCTGGAGGACTTCATAGCCAAGAAGGTGGAGCACAACCAGCGCACGCTGGATCCCAATTCCCCACGGGACTTCATTGACTCCTTTCTCATCCGCATGCAGGAGGAGGAGAAGAACCCCAACACGGAGTTCTACTTGAAAAACCTGGTGATGACCACGTTGAACCTCTTCATTGGGGGCACCGAGACCGTCAGCACCACCCTGCGCTATGGCTTCTTGCTGCTCATGAAGCACCCAGAGGTGGAGGCCAAGGTCCATGAGGAGATTGACAGAGTGATCGGCAAGAACCGGCAGCCCAAGTTTGAGGACCGGGCCAAGATGCCCTACATGGAGGCAGTGATCCACGAGATCCAAAGATTTGGAGACGTGATCCCCATGAGTTTGGCCCGCAGAGTCAAAAAGGACACCAAGTTTCGGGATTTCTTCCTCCCTAAGGGCACCGAAGTGTACCCTATGCTGGGCTCTGTGCTGAGAGACCCCAGTTTCTTCTCCAACCCCCAGGACTTCAATCCCCAGCACTTCCTGAATGAGAAGGGGCAGTTTAAGAAGAGTGATGCTTTTGTGCCCTTTTCCATCGGAAAGCGGAACTGTTTCGGAGAAGGCCTGGCCAGAATGGAGCTCTTTCTCTTCTTCACCACCGTCATGCAGAACTTCCGCCTCAAGTCCTCCCAGTCACCTAAGGACATTGACGTGTCCCCCAAACACGTGGGCTTTGCCACGATCCCACGAAACTACACCATGAGCTTCCTGCCCCGGTCGACCCATCATCATCATCATCATTGA
>2A13
ATGGCTCTGATGTCCGTTTGGCGTCAGCGTAAATCCCGTGGTAAACTGCCGCCGGGTCCGACCCCGCTGCCGTTCATCGGTAACTACCTGCAGCTGAACACCGAACAGATGTACAACTCCCTGATGAAAATCTCCGAACGTTACGGTCCGGTTTTCACCATCCACCTGGGTCCGCGTCGTGTTGTTGTTCTGTGCGGTCACGACGCTGTTAAAGAAGCTCTGGTAGACCAGGCTGAAGAATTCTCCGGTCGTGGTGAACAGGCTACCTTCGACTGGCTGTTCAAAGGTTACGGTGTTGCTTTCTCCAACGGTGAGCGCGCTAAACAGCTGCGTCGTTTCTCCATCGCTACCCTGCGTGGTTTCGGTGTTGGTAAACGTGGTATCGAAGAACGTATCCAGGAAGAAGCTGGTTTCCTGATCGACGCTCTGCGTGGCACCCACGGTGCTAACATCGACCCGACCTTCTTCCTGTCCCGTACCGTTTCCAACGTTATCTCCTCCATCGTTTTCGGTGACCGTTTCGACTACGAAGACAAAGAATTCCTGTCCCTGCTGCGTATGATGCTGGGTTCCTTCCAGTTCACCGCTACCTCCACCGGTCAGCTGTACGAAATGTTCTCCTCCGTTATGAAACACCTGCCGGGTCCGCAGCAGCAGGCTTTCAAAGAACTGCAGGGTCTGGAAGACTTCATCGCTAAAAAAGTTGAACACAACCAGCGTACCCTGGACCCGAACTCCCCGCGTGACTTCATCGACTCCTTCCTGATCCGCATGCAGGAAGAAGAAAAAAACCCGAACACCGAATTCTACCTGAAAAACCTGGTTATGACCACCCTGAACCTGTTCTTCGCGGGCACTGAGACCGTGAGCACCACCCTGCGCTACGGTTTCCTGCTGCTCATGAAGCACCCAGAGGTGGAGGCCAAGGTCCATGAGGAGATCGACCGTGTTATCGGTAAAAACCGTCAGCCGAAATTCGAAGACCGTGCTAAAATGCCGTACACCGAAGCTGTTATCCACGAAATCCAGCGTTTCGGTGACATGCTGCCGATGGGTCTGGCTCACCGTGTTAACAAAGACACCAAATTCCGTGACTTCTTCCTGCCGAAAGGTACCGAAGTTTTCCCGATGCTGGGTTCCGTTCTGCGTGACCCGCGTTTCTTCTCCAACCCGCGTGACTTCAACCCGCAGCACTTCCTGGACAAAAAAGGTCAGTTCAAAAAATCCGACGCTTTCGTTCCGTTCTCCATCGGTAAACGTTACTGCTTCGGTGAAGGTCTGGCTCGTATGGAACTGTTCCTGTTCTTCACCACCATCATGCAGAACTTCCGTTTCAAATCCCCGCAGTCCCCGAAAGACATCGACGTTTCCCCGAAACACGTTGGTTTCGCTACCATCCCGCGTAACTACACCATGTCCTTCCTGCCGCGTGCGTCGACCCATCATCATCATCATCATTGA

Cleave DNA into fragments between and bp.

Enable extensive search (Note: wait > 1hr)

Limit restriction enzymes to the following set (up to 50 enzymes; to search all enzymes, leave it blank):

Please cite: Huang W., Johnston W.A., Boden M. and Gillam E.M.J. (2016) ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries. Biotechniques, 60(2):91-94. (PMID: 26842355)

This service has been used 772 times

Developed by Weiliang Huang, PhD

University of Maryland, Baltimore, USA 2013-2014
University of Queensland, Australia 2007-2011

REcut 3.0

Automatic Restriction Enzyme Selector for DNA shuffling

part of RecutXover - A suite of computaional tools for accelerating the design, visualization and analysis of recombinatorial protein libraries

part of Re_cutX_over - A suite of computaional tools for accelerating the design, visualization and analysis of recombinatorial protein libraries