Reaction Details
Reaction: Thumb
5-amino-1-(5-phospho-β-D-ribosyl)imidazole + S-adenosyl-L-methionine → 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + carbon monoxide + 3 H+
Enzymes:
External Links:
ResourceLink
PseudoCyc Reaction ID:PYRIMSYN1-RXN
References:
  • Chatterjee A, Li Y, Zhang Y, Grove TL, Lee M, Krebs C, Booker SJ, Begley TP, Ealick SE (2008). "Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily." Nat Chem Biol 4(12);758-65. PMID: 18953358
  • Chatterjee A, Hazra AB, Abdelwahed S, Hilmey DG, Begley TP (2010). "A "Radical Dance" in Thiamin Biosynthesis: Mechanistic Analysis of the Bacterial Hydroxymethylpyrimidine Phosphate Synthase." Angew Chem Int Ed Engl 49(46);8653-6. PMID: 20886485
  • Martinez-Gomez NC, Poyner RR, Mansoorabadi SO, Reed GH, Downs DM (2009). "Reaction of AdoMet with ThiC generates a backbone free radical." Biochemistry 48(2);217-9. PMID: 19113839
  • Vander Horn PB, Backstrom AD, Stewart V, Begley TP (1993). "Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12." J Bacteriol 175(4);982-92. PMID: 8432721