Probable N-acetylmuramoyl-L-alanine amidase AmiA (PA5538)

Identification
Name: Probable N-acetylmuramoyl-L-alanine amidase AmiA
Synonyms: Not Available
Gene Name: amiA
Enzyme Class:
Biological Properties
General Function: Involved in N-acetylmuramoyl-L-alanine amidase activity
Specific Function: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
Cellular Location: Secreted (Potential)
KEGG Pathways:
  • Cationic antimicrobial peptide (CAMP) resistance pae01503
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb
N-Acetylmuramoyl-Ala + Water ↔ N-Acetyl-D-muramoate + L-Alanine
ReactionCard
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+Thumb → Thumb+Thumb
N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide + Water → L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanine + N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
Thumb+Thumb → Thumb+Thumb
N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide + Water → L-alanine-D-glutamate-meso-2,6-diaminoheptanedioate + N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
N-Acetylmuramoyl-Ala + Water ↔ N-Acetyl-D-muramoate + L-Alanine
ReactionCard
Thumb
EC 3.5.1.28
ReactionCard
PAMDB120226+PAMDB000142 → PAMDB120237+PAMDB120146
GlcNAc-1,6-anhMurNAc-L-Ala-γ-D-Glu-DAP-D-Ala + Water → N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate + L-Ala-γ-D-Glu-meso-DAP-D-Ala
ReactionCard
PAMDB120301+PAMDB000142 → PAMDB120125+PAMDB000063
N-acetylmuramoyl-L-alaninate + Water → N-acetylmuramate + L-Alanine
ReactionCard
Complex Reactions:
Thumb+Thumb → Thumb+Thumb
N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide + Water → L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanine + N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
Thumb+Thumb → Thumb+Thumb
N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide + Water → L-alanine-D-glutamate-meso-2,6-diaminoheptanedioate + N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000063L-AlanineMetaboCard
PAMDB001648L-alanine-D-glutamate-meso-2,6-diaminoheptanedioateMetaboCard
PAMDB001647L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanineMetaboCard
PAMDB001656N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acidMetaboCard
PAMDB001657N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptideMetaboCard
PAMDB001658N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptideMetaboCard
PAMDB001023N-Acetyl-D-muramoateMetaboCard
PAMDB001025N-Acetylmuramoyl-AlaMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
N-acetylmuramoyl-L-alanine amidase activity
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan catabolic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Locus tag: PA5538
Strand: -
Entrez Gene ID: 877724
Accession: NP_254225.1
GI: 15600731
Sequence start: 6230369
Sequence End: 6231562
Sequence Length: 1193
Gene Sequence: 
>PA5538
ATGAAGCGCCGTCGTCTCCTGCAAAGCCTGCTCGCCGGCCTGGCCCTCCAGCCGTTCCTAGCCGCCAGCGCCAATGTACGAATCCGCCAGGCCCGGGCCTGGAATTCCTCGGAAAGCTGGCGCCTGGTGCTGGAACTGGACGGCCCGCCGCGTTATCGAACCTTTTCCCTGCAGGCCCCCGAGCGGTTGATCCTCGACCTGCCCGACGCGCAATTGCTCGCAACGCTCTCCGAGCTACCGCTGGACGGCCCCGTGCGCGCCATACGCAGTGGCCAACTGGGTGGCGGCGGGACGCGCATCGTCCTCGATCTGCGCCAAGCGGTCAGGCCGCGCAGCTTCGCCCTGGAACCGCAGGCGGGAAAGGGCCATCGCCTGGTCCTGGACCTATATCCACAGGCGCTGGCGGCGCCCCCCGAGCCAATGCTGGCGCTGGCCCGCCCGCTACCCTCGACGGGCCGCGACATCCTCGTGGTGGTCGATGCCGGCCATGGCGGCAAGGACCCCGGCGCGGTCGGTAGCAAGGGCGAGCGCGAAAAGGACGTGGCGCTGGCCATCGCCCGCCTGCTGGCGCGGCGGATCGACCGTGAGAAAGGCTTCAAGGCGCGCCTGGTGCGCAACGGCGATGTGTTCGTCCCGCTGCGCAAGCGGCCGGAAGTGGCCCGGCGCTACAACGCCGACATGTTCGTTTCGGTGCACGCCGACGCGGCGCCGCGACTGACCGCCTCCGGCGCGTCGGTATATGCCCTCTCCGAGCATGGCGCCAGCTCGACCATGGCGCGCTGGATGGCCGAGCGCGAGAACGGCGCCGACCTGATCGGCAACAGCGGCAACCTGCCGCTACAGGGCCGCGACAAGGTACTCGCCGGGGTGATCCTCGACATGGCCCTGAGTTCGACCATCGCCAGCAGCCTGGACCTCGGCCACAGCGTTCTCGGCCAGTTGTCGGACGTCACCGCGCTGCACCAGAAGCGTGTCGAGCAGGCCGGCTTCGCCGTGCTCAAGTCGCCGGACATCCCTTCGATCCTGGTGGAGACCGGCTTCATCTCGAATCGCGGCGATTGTCGCCGGTTGATGGACGAACGCCATCGCCAGCGCCTGGCCGAGGCGATCTTCGCTGGCGTGCAGGGCTACTTCCGGCAACGCCCTCCCGCCGGCAGCTACCTGGCGGCGCTCAGCGCGCAGCCTCCCGGCTAG
Protein Properties
Protein Residues: 397
Protein Molecular Weight: 42.9 kDa
Protein Theoretical pI: 10.96
Hydropathicity (GRAVY score): -0.155
Charge at pH 7 (predicted): 16.66
Protein Sequence: 
>PA5538
MKRRRLLQSLLAGLALQPFLAASANVRIRQARAWNSSESWRLVLELDGPPRYRTFSLQAPERLILDLPDAQLLATLSELPLDGPVRAIRSGQLGGGGTRIVLDLRQAVRPRSFALEPQAGKGHRLVLDLYPQALAAPPEPMLALARPLPSTGRDILVVVDAGHGGKDPGAVGSKGEREKDVALAIARLLARRIDREKGFKARLVRNGDVFVPLRKRPEVARRYNADMFVSVHADAAPRLTASGASVYALSEHGASSTMARWMAERENGADLIGNSGNLPLQGRDKVLAGVILDMALSSTIASSLDLGHSVLGQLSDVTALHQKRVEQAGFAVLKSPDIPSILVETGFISNRGDCRRLMDERHRQRLAEAIFAGVQGYFRQRPPAGSYLAALSAQPPG
References
External Links:
Resource Link
Genome ID: PA5538
Entrez Gene ID: 877724
NCBI Protein ID: 15600731
General Reference: PaperBLAST - Find papers about PA5538 and its homologs