N-acetylmuramoyl-L-alanine amidase AmiB (PA4947)

Identification
Name: N-acetylmuramoyl-L-alanine amidase AmiB
Synonyms: Not Available
Gene Name: amiB
Enzyme Class:
Biological Properties
General Function: Involved in N-acetylmuramoyl-L-alanine amidase activity
Specific Function: Cell-wall hydrolase probably involved in cell-wall hydrolysis, septation or recycling
Cellular Location: Secreted (Potential)
KEGG Pathways:
  • Cationic antimicrobial peptide (CAMP) resistance pae01503
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb
N-Acetylmuramoyl-Ala + Water ↔ N-Acetyl-D-muramoate + L-Alanine
ReactionCard
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+Thumb → Thumb+Thumb
N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide + Water → L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanine + N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
Thumb+Thumb → Thumb+Thumb
N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide + Water → L-alanine-D-glutamate-meso-2,6-diaminoheptanedioate + N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
N-Acetylmuramoyl-Ala + Water ↔ N-Acetyl-D-muramoate + L-Alanine
ReactionCard
Thumb
EC 3.5.1.28
ReactionCard
PAMDB120226+PAMDB000142 → PAMDB120237+PAMDB120146
GlcNAc-1,6-anhMurNAc-L-Ala-γ-D-Glu-DAP-D-Ala + Water → N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate + L-Ala-γ-D-Glu-meso-DAP-D-Ala
ReactionCard
PAMDB120301+PAMDB000142 → PAMDB120125+PAMDB000063
N-acetylmuramoyl-L-alaninate + Water → N-acetylmuramate + L-Alanine
ReactionCard
Complex Reactions:
Thumb+Thumb → Thumb+Thumb
N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide + Water → L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanine + N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
Thumb+Thumb → Thumb+Thumb
N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide + Water → L-alanine-D-glutamate-meso-2,6-diaminoheptanedioate + N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000063L-AlanineMetaboCard
PAMDB001648L-alanine-D-glutamate-meso-2,6-diaminoheptanedioateMetaboCard
PAMDB001647L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanineMetaboCard
PAMDB001656N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acidMetaboCard
PAMDB001657N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptideMetaboCard
PAMDB001658N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptideMetaboCard
PAMDB001023N-Acetyl-D-muramoateMetaboCard
PAMDB001025N-Acetylmuramoyl-AlaMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
N-acetylmuramoyl-L-alanine amidase activity
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan catabolic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Locus tag: PA4947
Strand: -
Entrez Gene ID: 878469
Accession: NP_253634.1
GI: 15600140
Sequence start: 5551681
Sequence End: 5553108
Sequence Length: 1427
Gene Sequence: 
>PA4947
ATGGGTTGGGGCTTGCGTCTGCGGACGTTGTTGACTGGGGTGATGATTCTGCTGGCCTGCCAGGTCGGCGAGGTTCTGGCCGCCGCGCAAATCAAGAGCGTGCGGATCTGGCGCGCTCCGGACAACACCCGTCTGGTCTTCGATCTCTCCGGTCCGGTGCAGCACAGCCTGTTCACCCTGGCCGCTCCCAACCGTATCGTCATCGACGTCAGCGGCGCCCAACTGGCGACCCAGCTCAACGGTCTCAAGCTCGGCAACACTCCGATCACCGCGGTGCGCTCCGCCCAGCGCACGCCCAACGACCTGCGCATGGTCCTCGACCTGTCGGCGCAGGTCACCCCGAAAAGCTTCGTCCTGCCGCCCAACCAGCAATACGGCAACCGCCTGGTGGTCGATCTCTACGACCAGGGCGCCGACCTGACCCCGGACGTGCCCGCGACGCCGACCCCGAGCGTGCCGGTCACCCCGGTGACGCCGACCCAGCCGGTGGCCAAGCTGCCGCTGCCAACCAAGGGCGGCACCCGCGATATCGTCATCGCCATCGACGCCGGCCACGGCGGTGAAGACCCTGGCGCCCTTGGCCCGGGCGGGTTGCACGAGAAGAACATCACCCTGTCGATCGCTCGCGAGCTGCAACGCCAGATCAACCAGGTGCGCGGTTACCGGGCCGAGCTGACCCGTACCGGCGACTATTTCATCCCGCTGCGCAAGCGCACCGAGATCGCCCGCAAGAAGGGCGCCGACCTGTTCGTCTCGATCCATGCCGACGCCGCGCCGAGCCGCAGCGCCTTCGGTGCCTCGGTGTTCGCCCTGTCCGACCGCGGCGCCACTTCGGAAACCGCGCGCTGGCTGGCCGACAGCGAGAACCGTTCCGACCTGATCGGCGGCGATGGCAGCGTCAGCCTCGGCGACAAGGACCAGATGCTCGCCGGCGTGCTGCTCGACCTGTCGATGACCGCCACCCTCTCGTCGAGCCTGGATGTCGGCCACAAGGTACTGACCAATGTCGGCCGCATCACTTCGCTGCACAAGCGTCGTGTCGAGCAGGCCGGGTTCATGGTGCTGAAGTCGCCGGACATCCCATCGATCCTGGTGGAAACCGGCTTCATCTCGAACGTCAACGAGTCGCGCAAGCTGGCTAGCGCCAGCCATCAGCAGGCTTTGGCGCGTTCGATCACCAGCGGCATCCGCCAGTATTTCCAGCAGAGCCCGCCGCCCGGCACCTACATCGCCTCTTTGCGCGCCCAGGGCAAGCTCTCCATGGGGCCGCGCGAGCACGTGGTGCGGCCCGGAGAGACCCTGGCGATGATCGCCCAGCGCTATGAAGTGAGCATGGCCGCGCTGCGTAGCAGCAACAGCCTGTCCAGCGACAACCTGAAAGTCGGCCAGGCCCTGTCCATCCCGAGCACCGCCCTGGCGGCCCAGTGA
Protein Properties
Protein Residues: 475
Protein Molecular Weight: 50.7 kDa
Protein Theoretical pI: 10.36
Hydropathicity (GRAVY score): -0.091
Charge at pH 7 (predicted): 12.9
Protein Sequence: 
>PA4947
MGWGLRLRTLLTGVMILLACQVGEVLAAAQIKSVRIWRAPDNTRLVFDLSGPVQHSLFTLAAPNRIVIDVSGAQLATQLNGLKLGNTPITAVRSAQRTPNDLRMVLDLSAQVTPKSFVLPPNQQYGNRLVVDLYDQGADLTPDVPATPTPSVPVTPVTPTQPVAKLPLPTKGGTRDIVIAIDAGHGGEDPGALGPGGLHEKNITLSIARELQRQINQVRGYRAELTRTGDYFIPLRKRTEIARKKGADLFVSIHADAAPSRSAFGASVFALSDRGATSETARWLADSENRSDLIGGDGSVSLGDKDQMLAGVLLDLSMTATLSSSLDVGHKVLTNVGRITSLHKRRVEQAGFMVLKSPDIPSILVETGFISNVNESRKLASASHQQALARSITSGIRQYFQQSPPPGTYIASLRAQGKLSMGPREHVVRPGETLAMIAQRYEVSMAALRSSNSLSSDNLKVGQALSIPSTALAAQ
References
External Links:
Resource Link
Genome ID: PA4947
Entrez Gene ID: 878469
NCBI Protein ID: 15600140
General Reference: PaperBLAST - Find papers about PA4947 and its homologs