Poly(A) polymerase (PA4727)

Identification
Name: Poly(A) polymerase
Synonyms:
  • PAP
  • Plasmid copy number protein
Gene Name: pcnB
Enzyme Class:
Biological Properties
General Function: Involved in RNA binding
Specific Function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control
Cellular Location: Cytoplasmic
KEGG Pathways:
KEGG Reactions:
Thumb+RNA+RNA ↔ Thumb+RNA
Adenosine triphosphate + RNA + RNA ↔ Pyrophosphate + RNA
ReactionCard
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+RNA+RNA ↔ Thumb+RNA
Adenosine triphosphate + RNA + RNA ↔ Pyrophosphate + RNA
ReactionCard
Thumb+RNA(n) → Thumb+RNA(n+1)
Adenosine triphosphate + RNA(n) → Pyrophosphate + RNA(n+1)
ReactionCard
Thumb
ATP + an mRNA = an mRNA + diphosphate
ReactionCard
Complex Reactions:
Thumb+RNA(n) → Thumb+RNA(n+1)
Adenosine triphosphate + RNA(n) → Pyrophosphate + RNA(n+1)
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000148Adenosine triphosphateMetaboCard
PAMDB000633PyrophosphateMetaboCard
GO Classification:
Function
adenylyltransferase activity
binding
catalytic activity
nucleic acid binding
nucleotidyltransferase activity
polynucleotide adenylyltransferase activity
RNA binding
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
mRNA 3'-end processing
mRNA polyadenylation
mRNA processing
RNA metabolic process
RNA processing
Gene Properties
Locus tag: PA4727
Strand: +
Entrez Gene ID: 881610
Accession: NP_253415.1
GI: 15599921
Sequence start: 5309326
Sequence End: 5310729
Sequence Length: 1403
Gene Sequence: 
>PA4727
ATGCTGAAAAAGCTGATCCAGACCATTCGTTCCCCCCTGCGCCGTCCGCGCGCCGTCCGTACAACCCCGGAAGTCATCGGTAACAACCAGCACTCCCTGCGCCGGGACCAGTTCAGCCGGAACGCCGTCAAGGTCGTCGAGATCCTCCAGCGGGCCGGCTACCAGGCCTACGTGGTCGGGGGCTGCGTACGCGACCAGATGCTCGGCATCGCTCCCAAGGACTTCGATGTCGCCACCAGCGCCACGCCGGAGCAGGTCCGCGCCGAATTCCGCAACGCGCGGATCATCGGCCGCCGCTTCAAACTGGTACACGTGCACTTCGGCCGCGAGATCATCGAGGTCGCCACCTTCCGCGCCCATCATCCGGAAGGCCAGGACGATGGCGATAGCCGCGCGTCCAGCAACGAAAGCGGCCGCATCCTGCGCGACAACGTCTATGGCAGCCTGGAAGACGACGCCCAGCGCCGCGACTTCACCATCAATGCCCTGTACTTCGACGTCACCAGCGAGCGGCTGCTGGACTACGCCAATGGCGTTCACGACATCCGCAATCGCCTGATCCGCCTGATCGGCGATCCGGAGCAGCGTTATCTGGAAGACCCGGTGCGGATGCTGCGGGCGGTACGTTTCGCCGCCAAGCTCGACTTCGAGATCGAGAAGCACAGCGCCGCGCCGATTCGCCGGCTGGCCCCGTTGCTGCGGGAAATCCCTTCGGCGCGCCTGTTCGACGAGGTGCTCAAGCTGTTCCTCGCCGGCCGGGCCGAGCGCACTTTCGAGCTGCTGGTCGAGTACGAACTGTTCGCGCCGCTGTTCCCGGCCAGCGCCAAGGCACTGCAGGCCAACCCCGACTACACCGGCAAGCTGATCCGCCAGGCCCTGGCCAATACCGATGCGCGAATTCGCCAGGGCAAGCCGGTAACCCCGGCCTTCCTCTTCGCCGCACTGCTCTGGCCGGCCCTGCCGGCACGCGTCGCGCAGTTGCAGGAGAAAGGCATGCCGGCCATCCCGGCGATGCAGGAAGCCGCCCACGAACTGATCAGCGAACAGTGCCAGCGGATCGCCATTCCCAAGCGTTTCACCCTGCCGATCCGCGAGATCTGGGATATGCAGGAACGTCTGCCGCGACGCCAGGGCAAGCGCGCCGACCTGCTCCTGGAAAACCCGCGCTTCCGCGCCGGCTACGACTTCCTCCTGCTGCGCGAAAGTGCCGGCGAGGAAACCGAAGGACTCGGCCAATGGTGGACCGATTACCAGGAAGTCAGCGACAGCGAGCGGCGCAACATGATCCGCGACCTGGTCAGCCAGGAAGACGGCAGCGCCCCGCGCAAGCGTCGCCGCGGCGGCAACAGCGGCCGTCGGCGCCGCGGGCCGCGCAAGGAAGGCAGCGGCGGAAGCGGCGAATGA
Protein Properties
Protein Residues: 467
Protein Molecular Weight: 53.3 kDa
Protein Theoretical pI: 10.42
Hydropathicity (GRAVY score): -0.558
Charge at pH 7 (predicted): 16.89
Protein Sequence: 
>PA4727
MLKKLIQTIRSPLRRPRAVRTTPEVIGNNQHSLRRDQFSRNAVKVVEILQRAGYQAYVVGGCVRDQMLGIAPKDFDVATSATPEQVRAEFRNARIIGRRFKLVHVHFGREIIEVATFRAHHPEGQDDGDSRASSNESGRILRDNVYGSLEDDAQRRDFTINALYFDVTSERLLDYANGVHDIRNRLIRLIGDPEQRYLEDPVRMLRAVRFAAKLDFEIEKHSAAPIRRLAPLLREIPSARLFDEVLKLFLAGRAERTFELLVEYELFAPLFPASAKALQANPDYTGKLIRQALANTDARIRQGKPVTPAFLFAALLWPALPARVAQLQEKGMPAIPAMQEAAHELISEQCQRIAIPKRFTLPIREIWDMQERLPRRQGKRADLLLENPRFRAGYDFLLLRESAGEETEGLGQWWTDYQEVSDSERRNMIRDLVSQEDGSAPRKRRRGGNSGRRRRGPRKEGSGGSGE
References
External Links:
Resource Link
Genome ID: PA4727
Entrez Gene ID: 881610
NCBI Protein ID: 15599921
General Reference: PaperBLAST - Find papers about PA4727 and its homologs