Identification
Name: DNA polymerase III subunit alpha
Synonyms: Not Available
Gene Name: dnaE
Enzyme Class:
Biological Properties
General Function: Involved in 3'-5' exonuclease activity
Specific Function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+DNAThumb+DNA
Thumb+DNAThumb+DNA
Thumb+DNAThumb+DNA
Thumb+DNAThumb+DNA
Deoxynucleoside triphosphate+DNAThumb
Deoxynucleoside triphosphate + DNA ↔ Pyrophosphate
ReactionCard
SMPDB Reactions: Not Available
PseudoCyc/BioCyc Reactions:
Thumb+DNAThumb+DNA
Thumb+DNAThumb+DNA
Thumb+DNAThumb+DNA
Thumb+DNAThumb+DNA
Deoxynucleoside triphosphate+DNA(n)Thumb+DNA(n+1)
Deoxynucleoside triphosphate + DNA(n) → Pyrophosphate + DNA(n+1)
ReactionCard
Deoxynucleoside triphosphate+DNAThumb
Deoxynucleoside triphosphate + DNA ↔ Pyrophosphate
ReactionCard
Complex Reactions:
Deoxynucleoside triphosphate+DNA(n)Thumb+DNA(n+1)
Deoxynucleoside triphosphate + DNA(n) → Pyrophosphate + DNA(n+1)
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000412dATPMetaboCard
PAMDB000219dCTPMetaboCard
PAMDB000389dGTPMetaboCard
PAMDB000633PyrophosphateMetaboCard
PAMDB000346Thymidine 5'-triphosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
3'-5' exonuclease activity
binding
catalytic activity
DNA binding
DNA polymerase activity
DNA-directed DNA polymerase activity
exonuclease activity
hydrolase activity
hydrolase activity, acting on ester bonds
nuclease activity
nucleic acid binding
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA replication
macromolecule metabolic process
metabolic process
Gene Properties
Locus tag: PA3640
Strand: -
Entrez Gene ID: 880490
Accession: NP_252330.1
GI: 15598836
Sequence start: 4075157
Sequence End: 4078678
Sequence Length: 3521
Gene Sequence:
>PA3640
ATGACCGTATCCTTCGTTCACCTGCGTCTGCATACCGAATTCTCCCTGGTCGACGGCCTGGTCCGGGTCAAGCCCCTGGCCAAGGCAGTGGCAGGGCTGGGCATGCCGGCGGTGGCGGTCACCGACCAGAGCAACATGTGCTCGCTGGTGAAGTTCTACAAGACCGCCATGGGCGCCGGCATCAAGCCGATCTGCGGGGCCGACATCTGGCTCGCCAGCCGCGAGGAAGATGGCCCGCTCAGTCGCCTGAGCCTGCTGGCGATGAACGCGAAGGGTTATCGCAACCTCACCGAGCTGATTTCCCGCGGTTGGAGCGAAGGCCAGCGCAACGGCGAGATCATCATCGAACGCGACTGGGTCAAGGAGGCGGCGGAAGGCCTGATCGCGCTGTCCGCGGCCAAGGAGGGCGAGATCGGCCACGCGCTGCTCGACGGCGAGGAAGCCAAGGCCGAGGCGCTGTTGCGCGAGTGGATGGAGGTGTTCCCCGAGCGCTTCTACGTCGAAGTGCAACGTACCAGCCGGGTCAACGACGAGGAGCACCTCCACGCGGCGGTGGCTTTGGCGTCACGCTGCAACGCGCCGCTGGTGGCGACCAACGACGTGCGCTTCATCAAGCAGGAAGACTTCGAGGCCCACGAGACCCGCGTCTGCATCGGCGAGGGTCGGACCCTCGACGATCCGCGGCGGCCGCGTACCTATTCGGACCAGCAATACCTGAAGTCGCCGGCGGAAATGGCCGAGCTGTTCAGCGACCTGCCGGAAGCGTTGGAAAACACCGTCGAGATCGCCAAACGCTGCAACATCGAGGTGCAGCTGGGCAAGTACTTCCTGCCGGACTTCCCCACGCCCAATGGCATGGGCATCGACGACTACCTGCGGCACGCCTCCTTCGAAGGTCTCGAGGAGCGCCTGGAGGTGCTGTTGCCGAAGGACACCCCGGACTACGAGGCCAAGCGCCAGGTCTATGTCGATCGCCTGAACTTCGAACTCGATATCATCATCCAGATGGGTTTCCCCGGTTACTTCCTCATCGTGATGGACTTCATCAAGTGGGCGAAGAACAACGGCGTACCGGTAGGCCCCGGGCGGGGCTCGGGCGCCGGCTCGCTGGTGGCCTACGTGCTGAAGATCACCGACCTCGATCCGCTGGCCTATGACCTGCTGTTCGAACGTTTCCTCAACCCGGAACGGATCTCCATGCCCGACTTCGACGTCGACTTCTGCATGGAGGGCCGCGACCGGGTGATCGACTACGTGGCCGACGCCTACGGGCGCAACGCGGTGAGCCAGATCATCACCTTCGGCACCATGGCGGCGAAGGCGGTGGTACGCGACGTGGCGCGGGTCCAGGGCAAGTCCTACGGTCTCGCCGACCGGCTGTCGAAGATGATCCCGTTCGAAGTCGGCATGACCCTGGATAAGGCCTACGAGCAGGAAGAAATGCTCCGCGACTTCCTCAAGAGCGACGAGGAAGCCGCCGAGATCTGGGAGATGGCCCTCAAGCTGGAAGGCATCACCCGCGGTACCGGCAAGCACGCCGGTGGCGTGGTGATCGCGCCGACCAAGCTGACCGACTTCTCGCCGATCGCCTGCGACGAGGAAGGCGGCGGCCTGGTGACCCAGTTCGACAAGGACGACGTGGAGGCGGCCGGCCTGGTGAAGTTCGACTTCCTCGGCCTGCGGACCCTGACCATCATCAAGTGGGCGATGGAGATCATCAACCGCGAGCAGGCCAAGAAGGGCCTGGAGCCGGTGAACATCGACTTCATCCCGCTGGACGACAAGCCGACCTATTCGCTGCTGCAGAAGGCCGAGACCACTGCGGTGTTCCAGCTCGAATCGCGCGGCATGAAGGAGCTGATCAAGAAGCTCAAGCCGGACTGCCTGGAAGACCTCATCGCACTGGTGGCGCTGTTCCGCCCGGGTCCTCTGCAGTCGGGCATGGTGGACGACTTCATCAACCGCAAGCACGGTCGCGCCGAGCTGTCCTATCCGCACCCCGATTACCAGTACGCCGGCCTGGAGCCGGTGCTCAAGCCCACCTACGGCATCATCCTGTACCAGGAACAGGTGATGCAGATCGCCCAGGTCATGGCCGGCTATACCCTCGGTGGCGCGGACATGCTGCGCCGGGCGATGGGCAAGAAGAAGCCCGAGGAAATGGCCAAGCAGCGCGGCGGTTTCATCGAGGGTTGCAAGAACAACGGCATCGATGCCGACCTCGCGGGCAACATCTTCGACCTGGTGGAAAAATTCGCCGGCTACGGCTTCAACAAGTCGCACTCGGCAGCCTACGGCCTGGTCTCCTACCAGACCGCCTGGCTGAAGACCCACTTCCCGGCGCCGTTCATGGCCGCGGTACTCACCGCGGATATGCAGAACACCGACAAGGTGGTGACGCTGATCGAAGAATGCCGGCACATGAAGCTGCGCATCGTGGCGCCGGACGTGAACAACTCCGAGTTCCGCTTCACCGTCGACGACGATGGCCGGATCGTCTATGGCCTGGGCGCGATCAAGGGCGTCGGCGAGGGGCCGGTGGAGGCGATCACCGAATGCCGCGCCGAAGGCGGGCCGTTCAACACCCTGTTCGACTTCTGCGACCGCGTCGACCTGAAACGCATCAACAAGCGCACCCTGGAAGCGCTGATCCGCGCCGGTGCGCTGGATCGCCTGGGGCCGCACTACCACGACGAGCTAAAGGCCTACCAGGCTACCGTCGACCTCAACCGCGCCGTGCTGCTGGCGGCGATGGAGGAAGCGATCCAGGCCGCCGAGCAGACCGCGCGCAGCCACGACAGCGGGCACATGGACCTGTTCGGCGGAGTCTTCGCCGAGCCGGAGGCGGATGTCTACGCCAACCACCGCAAGGTCAAGGAGCTGACCCTCAAGGAACGCCTGAAGGGCGAGAAGGACACCCTCGGCCTGTACCTGACCGGCCATCCGATCGACGAGTACGAAGGCGAGGTGCGGCGCTTCGCCCGCCAGCGCATCGTCGAGCTGAAGCCGGCGCGCGATACCCAGACGGTGGCCGGGCTGATCGTCAACCTGCGGGTGATGAAGAACAAGAAGGGCGACAAGATGGGCTTCGTCACCCTCGACGACCGCTCCGGACGGATCGAGGCCTCGCTGTTCTCCGAAGCCTTCGCCGCGGCCCAGTCGCTGCTGCAGACCGACGCGCTGGTGGTGGTCGAGGGCGAGGTCAGCCAGGACGACTTCTCCGGCGGACTGCGCCTGCGCGCCAAGCGCGTGATGAGCCTGGAGGAGGCGCGTACCGGCCTGGCGGAGAGCCTGCGGATGAAGCTGCACGCCGACCTGTTGAAGGGCGATCGCCTGCGCTGGCTGGGCGAGCTGTTCAACCGCCATCGCGGTTCCTGCCCGATCACCCTGGACTACACCAGCGCCGACGCCAAGGCCCTGCTGCAGTTCGGCGAGAGCTGGCGGGTGGATCCGGCGGACGATCTGATCCAGGCATTGCGTGACCAGTTCGGGCGCGACAACGTCTTCCTCAATTATCGTTAA
Protein Properties
Protein Residues: 1173
Protein Molecular Weight: 130.9 kDa
Protein Theoretical pI: 5.07
Hydropathicity (GRAVY score): -0.237
Charge at pH 7 (predicted): -30.23
Protein Sequence:
>PA3640
MTVSFVHLRLHTEFSLVDGLVRVKPLAKAVAGLGMPAVAVTDQSNMCSLVKFYKTAMGAGIKPICGADIWLASREEDGPLSRLSLLAMNAKGYRNLTELISRGWSEGQRNGEIIIERDWVKEAAEGLIALSAAKEGEIGHALLDGEEAKAEALLREWMEVFPERFYVEVQRTSRVNDEEHLHAAVALASRCNAPLVATNDVRFIKQEDFEAHETRVCIGEGRTLDDPRRPRTYSDQQYLKSPAEMAELFSDLPEALENTVEIAKRCNIEVQLGKYFLPDFPTPNGMGIDDYLRHASFEGLEERLEVLLPKDTPDYEAKRQVYVDRLNFELDIIIQMGFPGYFLIVMDFIKWAKNNGVPVGPGRGSGAGSLVAYVLKITDLDPLAYDLLFERFLNPERISMPDFDVDFCMEGRDRVIDYVADAYGRNAVSQIITFGTMAAKAVVRDVARVQGKSYGLADRLSKMIPFEVGMTLDKAYEQEEMLRDFLKSDEEAAEIWEMALKLEGITRGTGKHAGGVVIAPTKLTDFSPIACDEEGGGLVTQFDKDDVEAAGLVKFDFLGLRTLTIIKWAMEIINREQAKKGLEPVNIDFIPLDDKPTYSLLQKAETTAVFQLESRGMKELIKKLKPDCLEDLIALVALFRPGPLQSGMVDDFINRKHGRAELSYPHPDYQYAGLEPVLKPTYGIILYQEQVMQIAQVMAGYTLGGADMLRRAMGKKKPEEMAKQRGGFIEGCKNNGIDADLAGNIFDLVEKFAGYGFNKSHSAAYGLVSYQTAWLKTHFPAPFMAAVLTADMQNTDKVVTLIEECRHMKLRIVAPDVNNSEFRFTVDDDGRIVYGLGAIKGVGEGPVEAITECRAEGGPFNTLFDFCDRVDLKRINKRTLEALIRAGALDRLGPHYHDELKAYQATVDLNRAVLLAAMEEAIQAAEQTARSHDSGHMDLFGGVFAEPEADVYANHRKVKELTLKERLKGEKDTLGLYLTGHPIDEYEGEVRRFARQRIVELKPARDTQTVAGLIVNLRVMKNKKGDKMGFVTLDDRSGRIEASLFSEAFAAAQSLLQTDALVVVEGEVSQDDFSGGLRLRAKRVMSLEEARTGLAESLRMKLHADLLKGDRLRWLGELFNRHRGSCPITLDYTSADAKALLQFGESWRVDPADDLIQALRDQFGRDNVFLNYR
References
External Links:
Resource Link
Genome ID: PA3640
Entrez Gene ID: 880490
NCBI Protein ID: 15598836
General Reference: PaperBLAST - Find papers about PA3640 and its homologs